You searched for: Protein Linking and Labeling Systems

Supplier: AAT BIOQUEST

iFluor® 555 PSA kit is a much superior replacement for Alexa Fluor 555 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.

Supplier: AAT BIOQUEST

iFluor® 647 PSA kit is a much superior replacement for Alexa Fluor 647 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.

Supplier: AAT BIOQUEST

iFluor® 532 WGA conjugate, like Alexa Fluor® 532 WGA conjugate, retains its ability to bind to sialic acid and N-acetylglucosaminyl residues, enhancing its utility in fluorescence imaging and analysis for various scientific investigations.

Supplier: AAT BIOQUEST

Although FITC is still the most popular fluorescent labelling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence.

Supplier: AAT BIOQUEST

iFluor® 680 WGA conjugate, like Alexa Fluor® 680 WGA conjugate, retains its ability to bind to sialic acid and N-acetylglucosaminyl residues, enhancing its utility in fluorescence imaging and analysis for various scientific investigations.

Supplier: Thermo Fisher Scientific

The Thermo Scientific™ Pierce™ MS-Compatible Magnetic IP Kit (Protein A/G) provides mass spectrometry-friendly reagents and an optimised protocol to enable highly effective and efficient immunoprecipitation and co-immunoprecipitation of target antigens upstream of LC-MS analysis.

Supplier: Thermo Fisher Scientific

Cross linkers are used for the identification of near-neighbour protein relationships and ligand-receptor interactions. Homobifunctional, amine-reactive NHS esters or imidates, and heterobifunctional, amine-reactive, photoactivatable phenyl azides are the most commonly used crosslinkers for these applications.

Supplier: AAT BIOQUEST

iFluor® 594 PSA kit is a much superior replacement for Alexa Fluor 594 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.

Supplier: Thermo Fisher Scientific

The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.

Supplier: Thermo Fisher Scientific

Photoactivatable Biotin, Biotin-LC-ASA , Psoralen-PEG3-Biotin and TFPA-PEG3-Biotin each contain a photoactivatable group. When exposed to UV light, they become activated and insert non specifically into nearby molecules. These reagents may be used to label proteins and peptides, but they are also useful in labeling DNA, RNA and other molecules that do not contain any readily reactive functional groups. Psoralen-PEG3-Biotin contains a reactive psoralen group and is
designed to efficiently label nucleic acids. The psoralen group intercalates into the helix of DNA, allowing it to label efficiently and selectively. The psoralen can also stack along with the bases of single-stranded DNA or RNA increasing labeling efficiency and selectivity.

Supplier: AAT BIOQUEST

iFluor® 350 PSA kit is a much superior replacement for Alexa Fluor 350 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.

Supplier: AAT BIOQUEST

iFluor® 350 PSA kit is a much superior replacement for Alexa Fluor 350 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.

Supplier: AAT BIOQUEST

iFluor® 488 PSA kit is a much superior replacement for Alexa Fluor 488 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.

Supplier: Thermo Fisher Scientific

The NHS-Azide and NHS-Phosphine Reagents are amine-reactive compounds for derivatising primary amines of proteins or amine-coated polymer surfaces. Once a protein or surface is azide- or phosphine-labelled, the two components are mixed for effective and stable conjugation. Phosphine groups react with azides via a Staudinger reaction to produce an aza-ylide intermediate that is trapped to form a stable, covalent amide bond. Because phosphines and azides are absent from biological systems, there is minimal background labelling of macromolecules found in cells or lysates.

Supplier: Thermo Fisher Scientific

The DyLight™ Specialty Dyes are fluorescent dyes with varying spectral characteristics, degrees of sulfonation, charges and hydrophobicities. These dyes are offered in their amine-reactive forms and are mainly derivatized with N-hydroxysuccinimide (NHS) esters, the simplest and most commonly used reactive group for labeling proteins. The large selection of near infrared (NIR) and infrared (IR) dyes may be used for in vivo and near-IR fluorescence (NIRF) applications.

Supplier: AAT BIOQUEST

iFluor® 594 PSA kit is a much superior replacement for Alexa Fluor 594 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.

Supplier: Thermo Fisher Scientific

The DyLight™ NHS Ester Quenchers are amine-reactive non-fluorescent dyes that use fluorescent resonance energy transfer (FRET) to quench fluorescent signals resulting from ultraviolet, visible and infrared light-excited fluorescent dyes. These dyes can be efficiently paired with many DyLight™ dyes and other common fluorescent dyes including fluorescein, rhodamine, cyanine and Texas Red® dye.

Supplier: AAT BIOQUEST

iFluor® 488 PSA kit is a much superior replacement for Alexa Fluor 488 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.

Supplier: AAT BIOQUEST

iFluor® 555 PSA kit is a much superior replacement for Alexa Fluor 555 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.

Supplier: AAT BIOQUEST

iFluor® 647 PSA kit is a much superior replacement for Alexa Fluor 647 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.

Supplier: Thermo Fisher Scientific

The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.

Supplier: Thermo Fisher Scientific

DyLight™ PEGylated dyes display absorption spectra ranging from 550 nm to 783 nm. These reagents fluoresce over a broad pH range, are more intense than Alexa Fluor® or Cy® dyes in many applications and match the output wavelengths of common fluorescence instrumentation. Additionally, the water solubility of the DyLight™ reagents allows a high dye-to-protein ratio without precipitation during conjugation.

Supplier: Thermo Fisher Scientific

The DyLight™ Long Stokes Shift Dyes are amine-reactive fluorescent dyes that produce long Stokes (LS) shifts of 80 to 145 nm in excitation/emission wavelengths. The DyLight™ LS dyes provide multicolour detection using a single excitation source in applications including flow cytometry, conjugation, DNA-sequencing, microscopy and fluorescence in situ hybridization (FISH). These dyes contain N-hydroxysuccinimide (NHS) esters, the most commonly used reactive group for labeling proteins. NHS esters react with primary amines on proteins and other molecules, forming a stable, covalent amide bond and releasing the NHS group.

Supplier: G-Biosciences

HOOK™ Biotin-Hydrazide and its long spacer arm equivalent, HOOK™ Biotin-LC-Hydrazide, are carbohydrate reactive reagents. HOOK™ carbohydrate reactive biotin reagents react with oxidised carbohydrate side chains. Some biotin reagents do not bind directly to the protein itself, but instead, conjugate to the carbohydrate residues of glycoproteins. The carbohydrate reactive biotin reagents contain hydrazides (-NH-NH₂) as a reactive group. The hydrazide reactions require carbonyl groups, such as aldehydes and ketones, which are formed by oxidative treatment of the carbohydrates. Hydrazides react spontaneously with carbonyl groups, forming a stable hydrazone bond. These reagents are particularly suitable for labelling and studying glycosylated proteins, such as antibodies and receptors.

Supplier: G-Biosciences

HOOK™ Biotin-PEG₂-Amine, and its long chain form, HOOK™ Biotin-PEG₃-Amine, are carboxyl reactive biotinylation reagents. These agents contain terminal amines and react with carboxyl groups found at the carboxyl termini, aspartate, and glutamate side chains. The reaction is mediated by a water-soluble carbodiimide (EDC). The carbodiimide activates the carboxyl group and reacts with the amines (-NH₂) on the biotinylation agent to form an amide bond. This reaction is rapid and takes just a few minutes to complete. Under these conditions, hydrazide-derivatives of biotin reagents may also react with the carboxyls.

Supplier: Merck Millipore (Calbiochem‎)

General proteolytic substrate. Provides a rapid, simple method for the detection of proteases in aqueous solutions.

Supplier: Biotium

Mix-n-Stain™ DNP antibody labelling kits utilise a technology that allows rapid antibody labelling with dinitrophenol without a purification step.

Supplier: Biotium

Biotinylated probes for detection of His-tagged proteins immobilised on nitrocellulose membranes.

Supplier: Merck Millipore (Calbiochem‎)

FluorSave™ reagent is an aqueous mounting medium for use in conjunction with fluorescent tracers such as fluorescein, rhodamine and phycobiliprotein probes.

Supplier: Biotium

Mix-n-Stain™ Digoxigenin antibody labelling kits utilise a technology that allows rapid antibody labelling with digoxigenin without a purification step.