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325 results for Protein Linking and Labeling Systems

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Protein Linking and Labeling Systems

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.

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Wheat Germ Agglutinin, XFD647 Labelled *XFD647 Same Structure to Alexa Fluor® 647*

Wheat Germ Agglutinin, XFD647 Labelled *XFD647 Same Structure to Alexa Fluor® 647*

Supplier: AAT BIOQUEST

XFD647, manufactured by AAT Bioquest, has an identical chemical structure to Alexa Fluor® 647, a trademark of ThermoFisher.

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ReadiLink™ xtra Rapid XFD594 Antibody Labelling Kit *BSA-Compatible, XFD594 Same Structure to Alexa Fluor™ 594* (2 Labellings)

ReadiLink™ xtra Rapid XFD594 Antibody Labelling Kit *BSA-Compatible, XFD594 Same Structure to Alexa Fluor™ 594* (2 Labellings)

Supplier: AAT BIOQUEST

XFD594 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 594 (Alexa Fluor® is the trademark of ThermoFisher).

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ReadiLink™ xtra Rapid XFD555 Antibody Labelling Kit *BSA-Compatible, XFD555 Same Structure to Alexa Fluor™ 555* (2 Labellings)

ReadiLink™ xtra Rapid XFD555 Antibody Labelling Kit *BSA-Compatible, XFD555 Same Structure to Alexa Fluor™ 555* (2 Labellings)

Supplier: AAT BIOQUEST

XFD555 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 555 (Alexa Fluor® is the trademark of ThermoFisher).

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Buccutite™ Rapid APC-Cy7 Tandem Antibody Labelling Kit

Supplier: AAT BIOQUEST

Production scale optimised for labelling 1 mg antibody per reaction. Buccutite™ rapid APC-Cy7 tandem antibody labelling kits, designed for large-scale production, provide a streamlined approach for labelling antibodies with PE, APC, PerCP and iFluor® tandem dyes.

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Buccutite™ Rapid APC-Cy5.5 Tandem Antibody Labelling Kit

Supplier: AAT BIOQUEST

Production scale optimised for labelling 1 mg antibody per reaction. Buccutite™ Rapid APC-Cy5.5 tandem antibody labelling kits, designed for large-scale production, provide a streamlined approach for labelling antibodies with PE, APC, PerCP and iFluor® tandem dyes.

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HIS Lite™ iFluor® 647 Tris NTA-Ni Complex

HIS Lite™ iFluor® 647 Tris NTA-Ni Complex

Supplier: AAT BIOQUEST

iFluor 647-Tris NTA compound is used as a sensitive fluorescent probe for detecting polyhistidine-labelled proteins in cells, solution and solid surfaces.

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ReadiLink™ xtra Rapid XFD647 Antibody Labelling Kit *BSA-Compatible, XFD647 Same Structure to Alexa Fluor™ 647* (2 Labelings)

ReadiLink™ xtra Rapid XFD647 Antibody Labelling Kit *BSA-Compatible, XFD647 Same Structure to Alexa Fluor™ 647* (2 Labelings)

Supplier: AAT BIOQUEST

XFD647 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 647 (Alexa Fluor® is the trademark of ThermoFisher).

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ReadiLink™ Rapid trFluor™ Tb Antibody Labelling Kit

Supplier: AAT BIOQUEST

Microscale optimised for labelling 50 µg antibody per reaction. Many biological compounds present in cells, serum or other biological fluids are naturally fluorescent, and thus the use of conventional, prompt fluorophores leads to serious limitations in assay sensitivity due to the high background caused by the autofluorescence of the biological molecules to be assayed.

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MitoROS™ 580 Kit for Detecting Reactive Oxygen Species (ROS) in Mitochondria

MitoROS™ 580 Kit for Detecting Reactive Oxygen Species (ROS) in Mitochondria

Supplier: AAT BIOQUEST

Oxidation of MitoROS™ 580 by superoxide generates red fluorescence.

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HIS Lite™ OG488-Tris NTA Chelator

Supplier: AAT BIOQUEST

HIS Lite™ OG488-Tris NTA Chelator is used as a sensitive green fluorescent probe for detecting polyhistidine-labelled proteins in combination with the addition of a heavy metal ion such as Ni²⁺ in cells, solution and solid surfaces.

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Buccutite™ Rapid trFluor™ D2 Acceptor Antibody Labelling Kit

Supplier: AAT BIOQUEST

Microscale optimised for labelling 100 µg antibody per reaction. Many biological compounds present in cells, serum, or other biological fluids are naturally fluorescent, and thus the use of conventional, prompt fluorophores leads to serious limitations in assay sensitivity due to the high background caused by the autofluorescence of the biological molecules to be assayed.

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Buccutite™ Rapid PE-Texas Red Tandem Antibody Labelling Kit

Supplier: AAT BIOQUEST

Production scale optimised for labelling 1 mg antibody per reaction. Buccutite™ Rapid PE-Texas Red tandem antibody labelling kits, designed for large-scale production, provide a streamlined approach for labelling antibodies with PE, APC, PerCP and iFluor® tandem dyes.

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ReadiLink™ Rapid mFluor™ Violet 510 Antibody Labelling Kit 'Microscale Optimised for Labelling 50 µg Antibody Per Reaction' (2 Labellings)

ReadiLink™ Rapid mFluor™ Violet 510 Antibody Labelling Kit 'Microscale Optimised for Labelling 50 µg Antibody Per Reaction' (2 Labellings)

Supplier: AAT BIOQUEST

AAT Bioquest's mFluor™ dyes are developed for flow cytometry-focused applications.

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ReadiLink™ Rapid mFluor™ Blue 570 Antibody Labelling Kit 'Microscale Optimised for Labelling 50 µg Antibody Per Reaction' (2 Labellings)

ReadiLink™ Rapid mFluor™ Blue 570 Antibody Labelling Kit 'Microscale Optimised for Labelling 50 µg Antibody Per Reaction' (2 Labellings)

Supplier: AAT BIOQUEST

AAT Bioquest's mFluor™ dyes are developed for flow cytometry-focused applications.

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Buccutite™ Poly-HRP Antibody Conjugation Kit

Buccutite™ Poly-HRP Antibody Conjugation Kit

Supplier: AAT BIOQUEST

Poly-HRP-antibody conjugates is considered to have the highest sensitivity in immunoassays where the sample is limited, or a target molecule is present at extremely low level.

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Fluorescence biotin quantitation kit, Pierce™

Fluorescence biotin quantitation kit, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Fluorescence Biotin Quantitation Kit requires only 10 μl of sample to accurately measure the biotinylation level of labeled antibodies and other biomolecules.

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HIS Lite™ Cy5 Bis NTA Chelator

Supplier: AAT BIOQUEST

Cy5 Bis NTA-Ni Chelator can be applied either to an SDS-PAGE gel or Western blot membrane for fluorescence imaging or used in living cells in combination with the addition of a heavy metal ion such as Ni²⁺.

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ReadiLink™ Rapid mFluor™ Violet 610 Antibody Labelling Kit

Supplier: AAT BIOQUEST

Microscale optimised for labeling 50 µg antibody per reaction. ReadiLink™ Rapid mFluor™ Violet 610 antibody labelling kits provide a quick and convenient method to label antibodies, proteins (<10 kDa), or other amine-containing biomolecules.

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HIS Lite™ Cy3 Bis NTA Chelator

HIS Lite™ Cy3 Bis NTA Chelator

Supplier: AAT BIOQUEST

Cy3 Bis NTA-Ni Chelator can be applied either to an SDS-PAGE gel or Western blot membrane for fluorescence imaging or used in living cells in combination with the addition of a heavy metal ion such as Ni²⁺.

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Crosslink immunoprecipitation (IP) kit, Pierce™

Crosslink immunoprecipitation (IP) kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Crosslink Immunoprecipitation (IP) Kit enables highly effective and efficient antigen immunoprecipitations by covalently crosslinking antibodies onto Protein A/G resin.

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ReadiLink™ Rapid XFD488 Antibody Labelling Kit 'XFD488 Same Structure to Alexa Fluor™ 488' (2×50 µg Labellings)

ReadiLink™ Rapid XFD488 Antibody Labelling Kit 'XFD488 Same Structure to Alexa Fluor™ 488' (2×50 µg Labellings)

Supplier: AAT BIOQUEST

XFD488 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 488 (Alexa Fluor® is the trademark of ThermoFisher).

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ReadiLink™ Rapid iFluor® Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)

ReadiLink™ Rapid iFluor® Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)

Supplier: AAT BIOQUEST

Although FITC is still the most popular fluorescent labelling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence.

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Antibody labelling kits, DyLight™

Antibody labelling kits, DyLight™

Supplier: Thermo Fisher Scientific

The DyLight™ Antibody Labeling Kits were specifically developed for fast, efficient labeling of antibodies. Two convenient kit formats are available to accommodate varied labeling requirements. The DyLight™ Antibody Labeling Kits contain all necessary components to perform three separate labeling reactions using 1 mg of IgG or similar quantities of other proteins.

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HIS Lite™ iFluor® 568 Tris NTA Chelator

Supplier: AAT BIOQUEST

iFluor® 568 Tris-NTA Chelator is used as a sensitive fluorescent probe for detecting polyhistidine-labelled proteins in combination with the addition of a heavy metal ion (such as Ni²⁺) in cells, solution and solid surfaces.

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Pierce™ PEGylated SMCC Crosslinkers, Heterobifunctional

Pierce™ PEGylated SMCC Crosslinkers, Heterobifunctional

Supplier: Thermo Fisher Scientific

Cross linkers are used for the identification of near-neighbour protein relationships and ligand-receptor interactions. Homobifunctional, amine-reactive NHS esters or imidates, and heterobifunctional, amine-reactive, photoactivatable phenyl azides are the most commonly used crosslinkers for these applications.

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PEGylated BM (bismaleimido) Crosslinkers, Homobifunctional, Pierce™

PEGylated BM (bismaleimido) Crosslinkers, Homobifunctional, Pierce™

Supplier: Thermo Fisher Scientific

Cross linkers are used for the identification of near-neighbour protein relationships and ligand-receptor interactions. Homobifunctional, amine-reactive NHS esters or imidates, and heterobifunctional, amine-reactive, photoactivatable phenyl azides are the most commonly used cross linkers for these applications.

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iFluor® 680-Wheat Germ Agglutinin (WGA) Conjugate

iFluor® 680-Wheat Germ Agglutinin (WGA) Conjugate

Supplier: AAT BIOQUEST

iFluor® 680 WGA conjugate, like Alexa Fluor® 680 WGA conjugate, retains its ability to bind to sialic acid and N-acetylglucosaminyl residues, enhancing its utility in fluorescence imaging and analysis for various scientific investigations.

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Amine reactive biotinylation reagents, EZ-Link™

Amine reactive biotinylation reagents, EZ-Link™

Supplier: Thermo Fisher Scientific

The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.

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Dye labeling kit (FITC), HOOK™

Supplier: G-Biosciences

FITC (fluorescein isothiocyanate) is a commonly used fluorescent label for proteins, as it contains the groups required for conjugating to amino, sulfhydryl, imidazoyl, tyrosyl or carbonyl groups of proteins. FITC has a molecular weight of 389, and excitation and emission wavelengths of 494 nm and 520 nm, respectively, therefore emitting green visible light.

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ReadiLink™ xtra Rapid XFD488 Antibody Labelling Kit *BSA-Compatible, XFD488 Same Structure to Alexa Fluor™ 488* (2 Labelings)

ReadiLink™ xtra Rapid XFD488 Antibody Labelling Kit *BSA-Compatible, XFD488 Same Structure to Alexa Fluor™ 488* (2 Labelings)

Supplier: AAT BIOQUEST

XFD488 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 488 (Alexa Fluor® is the trademark of ThermoFisher).

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