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324 results for Protein Linking and Labeling Systems

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Protein Linking and Labeling Systems

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.

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Dye labeling kit (FITC), HOOK™

Supplier: G-Biosciences

FITC (fluorescein isothiocyanate) is a commonly used fluorescent label for proteins, as it contains the groups required for conjugating to amino, sulfhydryl, imidazoyl, tyrosyl or carbonyl groups of proteins. FITC has a molecular weight of 389, and excitation and emission wavelengths of 494 nm and 520 nm, respectively, therefore emitting green visible light.

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ReadiLink™ iFluor® 488 FISH Fluorescence Imaging Kit

ReadiLink™ iFluor® 488 FISH Fluorescence Imaging Kit

Supplier: AAT BIOQUEST

The kit provides Taq DNA polymerase enzyme, which incorporates iFluor® 488-dUTPs in the target DNA through Polymerase Chain Reaction (PCR).

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ReadiLink™ xtra Rapid XFD488 Antibody Labelling Kit *BSA-Compatible, XFD488 Same Structure to Alexa Fluor™ 488* (2 Labelings)

ReadiLink™ xtra Rapid XFD488 Antibody Labelling Kit *BSA-Compatible, XFD488 Same Structure to Alexa Fluor™ 488* (2 Labelings)

Supplier: AAT BIOQUEST

XFD488 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 488 (Alexa Fluor® is the trademark of ThermoFisher).

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CytoWatch™ Wash-free Fluorescence Cell Imaging Buffer, 10X

CytoWatch™ Wash-free Fluorescence Cell Imaging Buffer, 10X

Supplier: AAT BIOQUEST

CytoWatch™ Wash-free fluorescence cell imaging buffer *10X* is a ready-to-use buffer optimised for fluorescence cell imaging.

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Buccutite™ Rapid APC-Cy7 Tandem Antibody Labelling Kit

Supplier: AAT BIOQUEST

Production scale optimised for labelling 1 mg antibody per reaction. Buccutite™ rapid APC-Cy7 tandem antibody labelling kits, designed for large-scale production, provide a streamlined approach for labelling antibodies with PE, APC, PerCP and iFluor® tandem dyes.

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Buccutite™ Rapid APC-Cy5.5 Tandem Antibody Labelling Kit

Supplier: AAT BIOQUEST

Production scale optimised for labelling 1 mg antibody per reaction. Buccutite™ Rapid APC-Cy5.5 tandem antibody labelling kits, designed for large-scale production, provide a streamlined approach for labelling antibodies with PE, APC, PerCP and iFluor® tandem dyes.

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HIS Lite™ OG488-Tris NTA Chelator

Supplier: AAT BIOQUEST

HIS Lite™ OG488-Tris NTA Chelator is used as a sensitive green fluorescent probe for detecting polyhistidine-labelled proteins in combination with the addition of a heavy metal ion such as Ni²⁺ in cells, solution and solid surfaces.

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Carboxyl reactive biotinylation reagents, EZ-Link™

Carboxyl reactive biotinylation reagents, EZ-Link™

Supplier: Thermo Fisher Scientific

Carboxyl groups, in the form of carboxy termini, aspartate residues or glutamate residues, can be targeted for biotin labeling using amine-derivatised biotin molecules. This reaction is mediated by a class of crosslinkers known as carbodiimides and results in the formation of an amide bond. The reaction with EDC, the most common carbodiimide crosslinker, is generally performed in an MES buffer at pH 4,5 to 5 and requires just minutes to complete.

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ReadiLink™ iFluor® 555 FISH Fluorescence Imaging Kit

ReadiLink™ iFluor® 555 FISH Fluorescence Imaging Kit

Supplier: AAT BIOQUEST

The kit provides Taq DNA polymerase enzyme which incorporates iFluor® 555-dUTPs in the target DNA through Polymerase Chain Reaction (PCR).

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iFluor® 350-Wheat Germ Agglutinin (WGA) Conjugate

iFluor® 350-Wheat Germ Agglutinin (WGA) Conjugate

Supplier: AAT BIOQUEST

iFluor® 350 WGA conjugate, like Alexa Fluor® 350 WGA conjugate, retains its ability to bind to sialic acid and N-acetylglucosaminyl residues, enhancing its utility in fluorescence imaging and analysis for various scientific investigations.

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ReadiLink™ xtra Rapid XFD555 Antibody Labelling Kit *BSA-Compatible, XFD555 Same Structure to Alexa Fluor™ 555* (2 Labellings)

ReadiLink™ xtra Rapid XFD555 Antibody Labelling Kit *BSA-Compatible, XFD555 Same Structure to Alexa Fluor™ 555* (2 Labellings)

Supplier: AAT BIOQUEST

XFD555 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 555 (Alexa Fluor® is the trademark of ThermoFisher).

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ReadiLink™ xtra Rapid XFD594 Antibody Labelling Kit *BSA-Compatible, XFD594 Same Structure to Alexa Fluor™ 594* (2 Labellings)

ReadiLink™ xtra Rapid XFD594 Antibody Labelling Kit *BSA-Compatible, XFD594 Same Structure to Alexa Fluor™ 594* (2 Labellings)

Supplier: AAT BIOQUEST

XFD594 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 594 (Alexa Fluor® is the trademark of ThermoFisher).

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Buccutite™ Rapid PE Antibody Labelling Kit

Supplier: AAT BIOQUEST

Production scale optimised for labelling 1 mg antibody per reaction. Buccutite™ Rapid PE antibody labelling kits, designed for large-scale production, offer a convenient and efficient method to label antibodies with PE, APC and iFluor® tandem dyes.

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ReadiLink™ xtra Rapid XFD647 Antibody Labelling Kit *BSA-Compatible, XFD647 Same Structure to Alexa Fluor™ 647* (2 Labelings)

ReadiLink™ xtra Rapid XFD647 Antibody Labelling Kit *BSA-Compatible, XFD647 Same Structure to Alexa Fluor™ 647* (2 Labelings)

Supplier: AAT BIOQUEST

XFD647 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 647 (Alexa Fluor® is the trademark of ThermoFisher).

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ReadiLink™ Rapid trFluor™ Tb Antibody Labelling Kit

Supplier: AAT BIOQUEST

Microscale optimised for labelling 50 µg antibody per reaction. Many biological compounds present in cells, serum or other biological fluids are naturally fluorescent, and thus the use of conventional, prompt fluorophores leads to serious limitations in assay sensitivity due to the high background caused by the autofluorescence of the biological molecules to be assayed.

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Photoreactive biotin reagents, HOOK™ Psoralen-PEO-Biotin

Supplier: G-Biosciences

Photoreactive agents, on exposure to ultraviolet light, become active and bind non specifically with neighbouring molecules. These reagents are suitable for labelling molecules that do not contain easily reactable functional groups. Photoreactive reagent HOOK™ Psoralen-PEO-Biotin reacts with and labels nucleic acids and protein molecules. When reacted with nucleic acids, it crosslinks with pyrimidine bases. Crosslinking does not interfere with hybridisation applications.

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MS-Compatible magnetic IP kit, streptavidin, Pierce™

Supplier: Thermo Fisher Scientific

The Thermo Scientific™ Pierce™ MS-Compatible Magnetic IP Kit (Streptavidin) provides mass spectrometry-friendly reagents and an optimised protocol to enable highly effective and efficient immunoprecipitation and co-immunoprecipitation of target antigens upstream of LC-MS.

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Buccutite™ Rapid PE-Cy7 Tandem Antibody Labelling Kit

Supplier: AAT BIOQUEST

Production scale optimised for labelling 1 mg antibody per reaction. Buccutite™ Rapid PE-Cy7 tandem antibody labelling kits, designed for large-scale production, provide a streamlined approach for labelling antibodies with PE, APC, PerCP and iFluor® tandem dyes.

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Buccutite™ Rapid trFluor™ D2 Acceptor Antibody Labelling Kit

Supplier: AAT BIOQUEST

Microscale optimised for labelling 100 µg antibody per reaction. Many biological compounds present in cells, serum, or other biological fluids are naturally fluorescent, and thus the use of conventional, prompt fluorophores leads to serious limitations in assay sensitivity due to the high background caused by the autofluorescence of the biological molecules to be assayed.

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Buccutite™ Rapid PE-Texas Red Tandem Antibody Labelling Kit

Supplier: AAT BIOQUEST

Production scale optimised for labelling 1 mg antibody per reaction. Buccutite™ Rapid PE-Texas Red tandem antibody labelling kits, designed for large-scale production, provide a streamlined approach for labelling antibodies with PE, APC, PerCP and iFluor® tandem dyes.

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ReadiLink™ iFluor® 488 Nick Translation dsDNA Labeling Kit

ReadiLink™ iFluor® 488 Nick Translation dsDNA Labeling Kit

Supplier: AAT BIOQUEST

It is compatible with a wide variety of sample materials, including bacterial artificial chromosome (BAC) DNA, human genomic DNA, purified PCR products, supercoiled and linearized plasmid DNA.

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ReadiLink™ DIG (Digoxigenin) Nick Translation dsDNA Labeling Kit

ReadiLink™ DIG (Digoxigenin) Nick Translation dsDNA Labeling Kit

Supplier: AAT BIOQUEST

It is compatible with a wide variety of sample materials, including bacterial artificial chromosome (BAC) DNA, human genomic DNA, purified PCR products, supercoiled and linearized plasmid DNA.

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ReadiLink™ Biotin Nick Translation dsDNA Labeling Kit

ReadiLink™ Biotin Nick Translation dsDNA Labeling Kit

Supplier: AAT BIOQUEST

It is compatible with a wide variety of sample materials, including bacterial artificial chromosome (BAC) DNA, human genomic DNA, purified PCR products, supercoiled and linearized plasmid DNA.

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HIS Lite™ Cy5 Bis NTA Chelator

Supplier: AAT BIOQUEST

Cy5 Bis NTA-Ni Chelator can be applied either to an SDS-PAGE gel or Western blot membrane for fluorescence imaging or used in living cells in combination with the addition of a heavy metal ion such as Ni²⁺.

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Fluorescence biotin quantitation kit, Pierce™

Fluorescence biotin quantitation kit, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Fluorescence Biotin Quantitation Kit requires only 10 μl of sample to accurately measure the biotinylation level of labeled antibodies and other biomolecules.

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Immobilised reductant column

Immobilised reductant column

Supplier: Thermo Fisher Scientific

Pierce™ Immobilised reductant column contains beaded resin to which an thiol-based reducing agent has been immobilised to enable solid-phase reduction of peptide and protein disulphide bonds.

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ReadiLink™ Rapid iFluor® Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)

ReadiLink™ Rapid iFluor® Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)

Supplier: AAT BIOQUEST

Although FITC is still the most popular fluorescent labelling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence.

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Speciality dyes, DyLight™

Speciality dyes, DyLight™

Supplier: Thermo Fisher Scientific

The DyLight™ Specialty Dyes are fluorescent dyes with varying spectral characteristics, degrees of sulfonation, charges and hydrophobicities. These dyes are offered in their amine-reactive forms and are mainly derivatized with N-hydroxysuccinimide (NHS) esters, the simplest and most commonly used reactive group for labeling proteins. The large selection of near infrared (NIR) and infrared (IR) dyes may be used for in vivo and near-IR fluorescence (NIRF) applications.

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ReadiLink™ xtra Rapid XFD750 Antibody Labelling Kit *BSA-Compatible, XFD750 Same Structure to Alexa Fluor™ 750* (2 Labellings)

ReadiLink™ xtra Rapid XFD750 Antibody Labelling Kit *BSA-Compatible, XFD750 Same Structure to Alexa Fluor™ 750* (2 Labellings)

Supplier: AAT BIOQUEST

XFD750 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 750 (Alexa Fluor® is the trademark of ThermoFisher).

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iFluor® 532-Wheat Germ Agglutinin (WGA) Conjugate

iFluor® 532-Wheat Germ Agglutinin (WGA) Conjugate

Supplier: AAT BIOQUEST

iFluor® 532 WGA conjugate, like Alexa Fluor® 532 WGA conjugate, retains its ability to bind to sialic acid and N-acetylglucosaminyl residues, enhancing its utility in fluorescence imaging and analysis for various scientific investigations.

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