You searched for: Protein Linking and Labeling Systems
Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.
Reactive dye conjugates
Supplier: Biotium
Reactive dyes containing an amine functional group.
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Antibody labelling kits, Mix-n-Stain™ CF™ Dye
Supplier: Biotium
Label your antibody with one of our superior CF® Dyes in 30 minutes without a purification step. Labelling tolerates many common buffer components including BSA and ascites.
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Biotin kits, Micro HOOK™
Supplier: G-Biosciences
The micro HOOK™ Biotin kits are designed to label small amounts of proteins, with each kit designed for 8 to 10 labellings of 50 to 250 µg protein/reaction.
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CF® Dye Azide
Supplier: Biotium
CF® Dyes with an azide group. Can be used to label or detect alkyne, BCN or cyclooctyne-containing molecules by fluorescence spectroscopy, microscopy or flow cytometry following copper-catalyzed azide-alkyne cycloaddition.
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CDI amine reactive resin
Supplier: G-Biosciences
CDI amine reactive agarose consists of 6% cross-linked agarose activated with CDI (1,1’-carbonyl diimidazole) to form reactive imidazole carbamates.
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CF™ Dye lectin conjugates
Supplier: Biotium
A lectin labelled with our superior fluorescent CF® dyes is an effective and widely used cell surface stain for yeast, fungi, and mammalian cells.
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Biotin conjugation estimation, HOOK™ BiotinQuant
Supplier: G-Biosciences
HOOK™ BiotinQuant is designed for the estimation of biotin conjugation. It measures biotin using HABA [4’-hydroxyazobenzene-2-carboxylic acid] dye. HABA binds with avidin at the biotin-binding site.
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Antibody labelling kits, Mix-n-Stain™ FITC
Supplier: Biotium
Mix-n-Stain™ FITC antibody labelling kits utilise a technology that allows rapid antibody labelling with green fluorescent dye (FITC) without a purification step.
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B-Phycoerythrin (BPE)
Supplier: Columbia Biosciences
Because of its high quantum yield, B-PE is considered the world’s brightest fluorophore.
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Dye conjugates dUTP CF®
Supplier: Biotium
CF® dye-dUTP can be used for TUNEL assay, microarray, or to synthesize labelled DNA probes for in situ hybridisation and nucleic acid blotting applications.
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Maxi fluorescent antibody labelling kits, Mix-n-Stain™, 1 mg labelling
Supplier: Biotium
The simplicity and convenience of Mix-n-Stain™ labelling, for conjugation of 1 mg IgG with fluorescent CF® dyes or other dyes.
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HRP-conjugate stabiliser, SeramunStab® STA2
Supplier: Apollo Scientific
Liquid product red odourless pH-value 7,3 to 7,5 Boiling point 102 °C. Stabiliser for a broad range of HRP conjugates.
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Small ligand labelling kits, Mix-n-Stain™ CF® Dye
Supplier: Biotium
Easily label SNAP-tag®, CLIP-tag™, HaloTag®, or other ligands with fluorescent dyes, no purification needed. Cell membrane-permeant and impermeant dye options for intracellular or surface targets.
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Annexin V Near IR CF® dye conjugates
Supplier: Biotium
Near-IR Annexin V fluorescent conjugates can be used to detect apoptotic cells and is suitable for in vivo imaging.
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Stabiliser for proteins and standards, SeramunStab® Stpur
Supplier: Apollo Scientific
Liquid product colourless to slightly yellow or greenish odourless pH 7,3 to 7,5 boiling point 104 °C. Stabiliser for controls and protein standards.
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CF™ azide reactive dyes
Supplier: Biotium
CF™ dye azide reacts with alkyne to form 1,2,3-triazole by copper(I) catalysed 1,3-dipolar Huisgen cycloaddition. It could also undergo Staudinger ligation with a phosphine containing compound.
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Stabiliser for sensitive biomolecules, SeramunStab® STB
Supplier: Apollo Scientific
Liquid product colourless to slightly yellow, freshly solutions opaleszent, odour weakly pH 7,3 to 7,5 boiling point 104 °C. Stabiliser for sensitive biomolecules like proteohormones or membrane proteins.
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Biotin SE protein labelling kit
Supplier: Biotium
This kit contains everything needed to label antibodies or other proteins with biotin succinimidyl ester (biotin SE). Each kit contains reagents for three labelling reactions of up to 1 mg protein per reaction.
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CF®DI Hydrazide
Supplier: Biotium
CF®DI dye hydrazides react with molecules containing aldehyde or ketone groups to form a hydrazone bond. The dyes are size- and charge-matched and do not significantly alter the molecular weight or pI of the labeled proteins. They are used for detection of protein carbonylation by fluorescence 2D-DIGE.
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Carbohydrate coupling resin
Supplier: G-Biosciences
Carbohydrate coupling resin is designed for the covalent immobilisation of carbohydrate containing molecules, including glycoproteins, to agarose beads. It is ideal for the coupling of polyclonal antibodies as it allows for the optimal orientation of the antibodies for affinity purification.
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CF® Dye BCN
Supplier: Biotium
CF® Dyes with a BCN group react with azides via a copper-free bioorthogonal reaction to label target molecules for surface staining of live cells or cell extracts. Membrane-permeant CF® Dye BCN conjugates for intracellular labelling reactions are also available.
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Dye tyramide, CF®
Supplier: Biotium
Fluorescent CF® dye tyramides are used for tyramide signal amplification (TSA) for increasing immunofluorescence sensitivity in multicolour immunocytochemistry (ICC), immunohistochemistry (IHC), or in situ hybridization (ISH).
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Tyramide amplification kit with HRP streptavidin and CF® dye or biotin tyramide
Supplier: Biotium
Kits for increasing immunofluorescence sensitivity using HRP-catalysed tyramide signal amplification with the choice of next-generation fluorescent CF® dyes or biotin.
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Carboxyl coupling resin
Supplier: G-Biosciences
Carboxyl coupling resin consists of 6% cross-linked agarose with covalently linked diaminodipropylamine (DADPA) to generate a free primary amine at the end of a long spacer arm. Molecules, including proteins, peptides and nucleic acids, are covalently coupled to the free primary amines, and the stable columns are ideal for affinity purification of antibodies and other interacting partners.
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ExoBrite™ True EV Membrane Stains
Supplier: Biotium
Lipophilic membrane dyes designed specifically for EV research and the best choice for general pan-EV staining.
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Bilirubin conjugate, sodium salt
Supplier: Cayman Chemical
Bilirubin conjugate is a stable, water-soluble ditaurate derivative of bilirubin meant to mimic endogenous bilirubin glucuronide derivatives.
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HRP-conjugate stabiliser, SeramunStab® STNP
Supplier: Apollo Scientific
Liquid product red odourless pH 6,7 to 6,9. Protein free Stabiliser. Enzyme activity preservation by special chemistry.
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Maackia amurensis Lectin (MAA/MAL II) (FITC)
Supplier: US Biological
Maackia amurensis Lectin (MAA/MAL II) is labelled with fluorescein isothiocyanate (FITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin.
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HRP - conjugate stabiliser, SeramunStab® STM
Supplier: Apollo Scientific
Liquid product orange to red odourless pH 6,4 to 6,6. Stabiliser for conjugates with monomeric HRP. Preservation of very low conjugate concentrations.
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Tyramide amplification kit with HRP goat, anti-rabbit and CF® dye or biotin tyramide
Supplier: Biotium
Kits for increasing immunofluorescence sensitivity using HRP-catalysed tyramide signal amplification with the choice of next-generation fluorescent CF® dyes or biotin.