You searched for: Protein Linking and Labeling Systems
Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.
VWR® Antibody signal enhancer
Supplier: VWR Chemicals
Antibody Signal Enhancer is a Western blotting antibody diluent that increases intensity of protein detection up to 5-fold compared to conventional blotting.
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SeramunBlock
Supplier: Apollo Scientific
Combined Stabiliser/ blocking solution for solid phase immobilised proteins effecting protein stabilisation and blocking of free surfaces.
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Antibody labelling kits, Mix-n-Stain™ CF™ Dye
Supplier: Biotium
Label your antibody with one of our superior CF® Dyes in 30 minutes without a purification step. Labelling tolerates many common buffer components including BSA and ascites.
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femtoCHROMO™- AP Substrate for chromogenic detection
Supplier: G-Biosciences
A ready to use modified BCIP (5-Bromo-4-Chloro-3’-Indolyphosphate p-Toluidine salt) and NBT (Nitro-Blue Tetrazolium Chloride) substrate that generates a black-purple insoluble precipitate in the presence of alkaline phosphatase.
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CF™ dye bovine serum albumin conjugates
Supplier: Biotium
Fluorescent bovine serum albumin (BSA) conjugates are commonly used as tracers in applications where physical dimensions are important. The conjugates can also be used for quantitative studies of electroporation, measurement of plasma volume in rats, and endocytosis studies. CF™ dye BSA conjugates are labelled with fluorescent CF™ dyes, which offer advantages in brightness and photostability compared to other fluorescent dyes.
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CF® Dye cAMP
Supplier: Biotium
Fluorescently labelled cAMP analogs that can be used to probe cAMP receptors.
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R-Phycoerythrin (RPE)
Supplier: Columbia Biosciences
Many applications and instruments were developed specifically for R-phycoerythrin.
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Coelenterazine Substrates for Renilla Luciferase and Apoaequorin
Supplier: Biotium
Coelenterazine and its analogs are luminescent enzyme substrates for apoaequorin and Renilla luciferase.
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CF™ dye streptavidin and monoclonal mouse anti-biotin conjugates
Supplier: Biotium
CF™ dyes are superior to other fluorescent dyes for protein labelling due to advantages in brightness, photostability and water solubility.
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Tyramide amplification buffer plus
Supplier: Biotium
An optimized buffer with enhanced sensitivity for tyramide signal amplification of immunostaining in cells or tissue sections.
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Antibody labelling kits, Mix-n-Stain™ FITC
Supplier: Biotium
Mix-n-Stain™ FITC antibody labelling kits utilise a technology that allows rapid antibody labelling with green fluorescent dye (FITC) without a purification step.
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HRP-conjugate stabiliser, SeramunStab® STA2
Supplier: Apollo Scientific
Liquid product red odourless pH-value 7,3 to 7,5 Boiling point 102 °C. Stabiliser for a broad range of HRP conjugates.
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ExoBrite™ True EV Membrane Stains
Supplier: Biotium
Lipophilic membrane dyes designed specifically for EV research and the best choice for general pan-EV staining.
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Wheat germ agglutinin (WGA) conjugates
Supplier: Biotium
A wheat germ agglutinin (WGA) lectin labelled with Biotium’s CF® Dyes or HRP. It is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine moieties of glycoproteins. As a result, WGA conjugates will label yeast bud scars and the cell membrane of gram-positive bacteria and mammalian cells.
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femtoCHROMO™- HRP Substrate for chromogenic detection
Supplier: G-Biosciences
A ready to use modified TMB (3, 3’, 5, 5’-Tetramethylbenzidine) substrate is used that generates a dark blue precipitate in the presence of horseradish peroxidase.
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Mix-n-Stain™ Cyanine dye antibody labelling kits
Supplier: Biotium
Revolutionary technology allows antibody labelling with Cyanine 555 (Cy®3) or Cyanine 647 (Cy®5) in 30 minutes without a purification step. The labelling procedure tolerates many common buffer components and antibody stabilisers.
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HRP - conjugate stabiliser, SeramunStab® STC
Supplier: Apollo Scientific
Liquid product red odourless pH-value 7,2 to 7,4 boiling point 103 °C. Optimised for background reduction.
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CF® Dye dCTP conjugates
Supplier: Biotium
CF® dye-dCTP can be used to synthesise probes for in situ hybridisation, microarray, or to synthesise labelled DNA probes for in situ hybridisation and nucleic acid blotting applications.
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Biotin reagents, OneQuant™ HOOK™
Supplier: G-Biosciences
The OneQuant™ format prevents loss of reagent due to repeated weighing as each vial contains only 1 - 2 mg HOOK™ Biotin Reagent.
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CF™ azide reactive dyes
Supplier: Biotium
CF™ dye azide reacts with alkyne to form 1,2,3-triazole by copper(I) catalysed 1,3-dipolar Huisgen cycloaddition. It could also undergo Staudinger ligation with a phosphine containing compound.
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Reactive quencher dyes
Supplier: Biotium
Reactive quenchers that act as acceptors in fluorescence resonance energy transfer (FRET) applications.
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Avidin, biotin, protein A/G and streptavidin reagents
Supplier: Rockland Immunochemicals
Avidin is biotin-binding protein, tetramer and suitable for used as a antigen, control and standard in assays. Avidin fluorescein, rhodamine, alkaline phosphatase, Texas Red™ conjugated are suitable for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent Western blotting. Avidin conjugated with glucose oxidase and peroxidase are useful as a detection reagent for primary antibodies conjugated with biotin and also can be utilised in Western blotting and ELISA experiments with proper substrates.
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Biotin kits, Micro HOOK™
Supplier: G-Biosciences
The micro HOOK™ Biotin kits are designed to label small amounts of proteins, with each kit designed for 8 to 10 labellings of 50 to 250 µg protein/reaction.
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Maxi fluorescent antibody labelling kits, Mix-n-Stain™, 1 mg labelling
Supplier: Biotium
The simplicity and convenience of Mix-n-Stain™ labelling, for conjugation of 1 mg IgG with fluorescent CF® dyes or other dyes.
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B-Phycoerythrin (BPE)
Supplier: Columbia Biosciences
Because of its high quantum yield, B-PE is considered the world’s brightest fluorophore.
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Dye conjugates dUTP CF®
Supplier: Biotium
CF® dye-dUTP can be used for TUNEL assay, microarray, or to synthesize labelled DNA probes for in situ hybridisation and nucleic acid blotting applications.
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Biotin SE protein labelling kit
Supplier: Biotium
This kit contains everything needed to label antibodies or other proteins with biotin succinimidyl ester (biotin SE). Each kit contains reagents for three labelling reactions of up to 1 mg protein per reaction.
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HRP-conjugate stabiliser, SeramunStab® STNP
Supplier: Apollo Scientific
Liquid product red odourless pH 6,7 to 6,9. Protein free Stabiliser. Enzyme activity preservation by special chemistry.
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Bilirubin conjugate, sodium salt
Supplier: Cayman Chemical
Bilirubin conjugate is a stable, water-soluble ditaurate derivative of bilirubin meant to mimic endogenous bilirubin glucuronide derivatives.
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Calmodulin Resin Kit for CBP purification
Supplier: G-Biosciences
The Calmodulin Resin Kit is for the affinity purification of calmodulin binding proteins (CBP), including recombinant proteins with a CBP tag and calmodulin-regulated proteins in eukaryotic cells. The resin is 4% agarose coupled to calmodulin and has a ligand density of approximately 1 mg calmodulin/ml resin.