Protein Linking and Labeling Systems

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.

AP-conjugate stabiliser, SeramunStab® STAP

Supplier: Apollo Scientific

Liquid product colourless odourless pH-value 5,9 to 6,1, Stabiliser for very low concentrated AP conjugates. Suitable for proteins resistant to pH values <6,0.

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5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 21-30, Abcam

Supplier: Abcam

Oligonucleotide conjugation / labeling in < 4 hrs with 30 secs hands-on time using 5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 21-30 ab270709. 100% antibody recovery.

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Streptavidin AZDye™ 488 Monovalent Antibody Labeling Kit, Abcam

Supplier: Abcam

AZdye 488 conjugation / labeling in < 4 hrs with 30 secs hands-on time using Streptavidin AZDye™ 488 Monovalent Antibody Labeling Kit ab272187. 100% antibody recovery.

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148Nd Metal Conjugation Kit - Lightning-Link®, Abcam

Supplier: Abcam

Labeling in < 4 hrs with 30 secs hands-on time using 148Nd Metal Conjugation Kit - Lightning-Link® ab269832. 100% antibody recovery.

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141Pr Metal Conjugation Kit - Lightning-Link®, Abcam

Supplier: Abcam

Labeling in < 4 hrs with 30 secs hands-on time using 141Pr Metal Conjugation Kit - Lightning-Link® ab269826. 100% antibody recovery.

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CF® Dye hydrazide

Supplier: Biotium

Fluorescent CF® Dye hydrazides are bright, extremely water-soluble, nontoxic molecules, useful as aldehyde-fixable cell tracers. They can also be used to label aldehyde or ketone groups of polysaccharides, carbohydrates or glycoproteins.

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Tyramide amplification kit with HRP goat, anti-rabbit and CF® dye or biotin tyramide

Supplier: Biotium

Kits for increasing immunofluorescence sensitivity using HRP-catalysed tyramide signal amplification with the choice of next-generation fluorescent CF® dyes or biotin.

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Phalloidin conjugates

Supplier: Biotium

Phalloidin is a toxin isolated from the deadly Amanita phalloides mushroom. It is a bicyclic peptide that binds specifically to F-actin. Fluorescent conjugates of phalloidin are convenient tools to investigate the distribution of F-actin in fixed and permeabilised cells or tissue sections. One unit (U) of phallodin is sufficient to stain one sample (200 µl staining volume). A highly selective actin filament stain for fixed and permeabilised cells. Labeled phalloidins have similar affinity for both large and small filaments.

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Annexin V conjugates

Supplier: Biotium

Annexin V fluorescent conjugates can be used to detect apoptotic cells. Annexin V is available conjugated to a wide variety of dyes.

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Antibody labelling kits, Mix-n-Stain™ Biotin

Supplier: Biotium

Mix-n-Stain™ Biotin antibody labelling kits utilise a technology that allows rapid antibody labelling with biotin without a purification step.

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TripleStain IHC kit: M&M&R on rodent tissue (DAB, HRP/Green & AP/Red), Abcam

Supplier: Abcam

The TripleStain IHC kit (ab183292) is designed to use with two mouse antibodies and one rabbit antibody (user supplied) to detect three distinct antigens on a single mouse/rat tissue or cell sample.

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Reactive biotin compounds

Supplier: Biotium

Biotium offers reactive biotin compounds with a variety of reactive functional groups.

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TripleStain IHC Kit: R&R&M on rodent tissue (DAB , AP/Red & HRP/Green), Abcam

Supplier: Abcam

Abcam's TripleStain IHC Kit (ab183295) is designed to be uses with user-supplied one mouse and two rabbit primary antibodies to detect three distinct antigens on a single mouse/rat tissue or cell sample.