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Protein Linking and Labeling Systems

Protein Linking and Labeling Systems

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.

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Streptavidin AZDye™ 488 Monovalent Antibody Labeling Kit, Abcam

Supplier: Abcam

AZdye 488 conjugation / labeling in < 4 hrs with 30 secs hands-on time using Streptavidin AZDye™ 488 Monovalent Antibody Labeling Kit ab272187. 100% antibody recovery.

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5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 21-30, Abcam

Supplier: Abcam

Oligonucleotide conjugation / labeling in < 4 hrs with 30 secs hands-on time using 5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 21-30 ab270709. 100% antibody recovery.

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CF™ azide reactive dyes

Supplier: Biotium

CF™ dye azide reacts with alkyne to form 1,2,3-triazole by copper(I) catalysed 1,3-dipolar Huisgen cycloaddition. It could also undergo Staudinger ligation with a phosphine containing compound.

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Avidin, biotin, protein A/G and streptavidin reagents

Avidin, biotin, protein A/G and streptavidin reagents

Supplier: Rockland Immunochemicals

Avidin is biotin-binding protein, tetramer and suitable for used as a antigen, control and standard in assays. Avidin fluorescein, rhodamine, alkaline phosphatase, Texas Red™ conjugated are suitable for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent Western blotting. Avidin conjugated with glucose oxidase and peroxidase are useful as a detection reagent for primary antibodies conjugated with biotin and also can be utilised in Western blotting and ELISA experiments with proper substrates.

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Mix-n-Stain™ Cyanine dye antibody labelling kits

Mix-n-Stain™ Cyanine dye antibody labelling kits

Supplier: Biotium

Revolutionary technology allows antibody labelling with Cyanine 555 (Cy®3) or Cyanine 647 (Cy®5) in 30 minutes without a purification step. The labelling procedure tolerates many common buffer components and antibody stabilisers.

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141Pr Metal Conjugation Kit - Lightning-Link®, Abcam

Supplier: Abcam

Labeling in < 4 hrs with 30 secs hands-on time using 141Pr Metal Conjugation Kit - Lightning-Link® ab269826. 100% antibody recovery.

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HRP - conjugate stabiliser, SeramunStab® STC

Supplier: Apollo Scientific

Liquid product red odourless pH-value 7,2 to 7,4 boiling point 103 °C. Optimised for background reduction.

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Cholera Toxin Subunit B (Recombinant)

Supplier: Biotium

This product is recombinant Cholera Toxin Subunit B expressed from E.coli cells. It is supplied lyophilised and binds GM1 ganglioside.

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DoubleStain IHC Kit: M&M on rodent tissue (DAB & AP/Red), Abcam

Supplier: Abcam

Abcam's Doublestain IHC Kit (ab183273) is designed to use with two user supplied mouse antibodies to detect two distinct antigens on mouse and rat tissue or cell samples.

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560/585 nm Extracellular Vesicle Membrane Staining Kit, Abcam

560/585 nm Extracellular Vesicle Membrane Staining Kit, Abcam

Supplier: Abcam

Labeling in < 4 hrs with 30 secs hands-on time using 560/585 nm Extracellular Vesicle Membrane Staining Kit ab314687. 100% antibody recovery.

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DoubleStain IHC Kit: G&M on human tissue (Green/HRP & AP/Red), Abcam

Supplier: Abcam

Abcam's DoubleStain IHC kit (ab183272) is designed to use with user supplied goat and mouse antibodies to detect two distinct antigens on human tissue or cell samples.

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Biotin reagents, OneQuant™ HOOK™

Supplier: G-Biosciences

The OneQuant™ format prevents loss of reagent due to repeated weighing as each vial contains only 1 - 2 mg HOOK™ Biotin Reagent.

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B-Phycoerythrin Conjugation Kit - Lightning-Link®, Abcam

Supplier: Abcam

Labeling in < 4 hrs with 30 secs hands-on time using B-Phycoerythrin Conjugation Kit - Lightning-Link® ab102871. 100% antibody recovery.

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femtoCHROMO™- HRP Substrate for chromogenic detection

femtoCHROMO™- HRP Substrate for chromogenic detection

Supplier: G-Biosciences

A ready to use modified TMB (3, 3’, 5, 5’-Tetramethylbenzidine) substrate is used that generates a dark blue precipitate in the presence of horseradish peroxidase.

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Streptavidin Gold Conjugate (40nm, 10 OD), Abcam

Supplier: Abcam

Labeling in < 4 hrs with 30 secs hands-on time using Streptavidin Gold Conjugate (40nm, 10 OD) ab186864. 100% antibody recovery.

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Atto 390 Conjugation Kit (Fast) - Lightning-Link®, Abcam

Supplier: Abcam

Labeling in < 20 mins with 30 secs hands-on time using Atto 390 Conjugation Kit (Fast) - Lightning-Link® ab269895. 100% antibody recovery.

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