Protein Linking and Labeling Systems
Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.
CDI amine reactive resin
Supplier: G-Biosciences
CDI amine reactive agarose consists of 6% cross-linked agarose activated with CDI (1,1’-carbonyl diimidazole) to form reactive imidazole carbamates.
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Antibody Purification Kit (Protein A), Abcam
Supplier: Abcam
Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions with enzymes or fluorophores. ab102784 quickly removes these contaminants.
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APC Conjugation Kit - Lightning-Link®, Abcam
Supplier: Abcam
APC conjugation / labeling in < 4 hrs with 30 secs hands-on time using APC Conjugation Kit ab201807. 100% antibody recovery. - Cited in over 50 publications - APC labeled antibody is ready for use in applications like WB, ELISA, and IHC, with no need for further purification - Compatible with most standard antibody formulations - Scalable;use the same conjugation method from 10µg-100mg of antibody - Use Lightning-Link® conjugation with over 45 labels, including Alexa Fluor®, DyLight®, tandem dyes, enzymes, oligonucleotides, and gold nanoparticles
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Fixable Viability Dye (750/777), Abcam
Supplier: Abcam
Labeling in < 4 hrs with 30 secs hands-on time using Fixable Viability Dye (750/777) ab314682. 100% antibody recovery.
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Biotin picolyl azide
Supplier: Biotium
Biotin picolyl azide reacts with alkyne to form 1,2,3-triazole by 1,3-dipolar Huisgen cycloaddition through the use of a much lower copper (I) concentration without sacrificing reaction efficiency.
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171Yb Metal Conjugation Kit - Lightning-Link®, Abcam
Supplier: Abcam
Labeling in < 4 hrs with 30 secs hands-on time using 171Yb Metal Conjugation Kit - Lightning-Link® ab269850. 100% antibody recovery.
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Latex Conjugation Kit - 400nm Red, Abcam
Supplier: Abcam
Labeling in < 4 hrs with 30 secs hands-on time using Latex Conjugation Kit - 400nm Red ab269893. 100% antibody recovery.
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Europium Conjugation Kit, Abcam
Supplier: Abcam
Europium conjugation / labeling in < 4 hrs with 30 secs hands-on time using Europium Conjugation Kit ab269889. 100% antibody recovery.
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153Eu Metal Conjugation Kit - Lightning-Link®, Abcam
Supplier: Abcam
Labeling in < 4 hrs with 30 secs hands-on time using 153Eu Metal Conjugation Kit - Lightning-Link® ab269836. 100% antibody recovery.
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Europium-Streptavidin, Abcam
Supplier: Abcam
Labeling in < 4 hrs with 30 secs hands-on time using Europium-Streptavidin ab270228. 100% antibody recovery.
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142Nd Metal Conjugation Kit - Lightning-Link®, Abcam
Supplier: Abcam
Labeling in < 4 hrs with 30 secs hands-on time using 142Nd Metal Conjugation Kit - Lightning-Link® ab269827. 100% antibody recovery.
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160Gd Metal Conjugation Kit - Lightning-Link®, Abcam
Supplier: Abcam
Labeling in < 4 hrs with 30 secs hands-on time using 160Gd Metal Conjugation Kit - Lightning-Link® ab269841. 100% antibody recovery.
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Avidin, biotin, protein A/G and streptavidin reagents
Supplier: Rockland Immunochemicals
Avidin is biotin-binding protein, tetramer and suitable for used as a antigen, control and standard in assays. Avidin fluorescein, rhodamine, alkaline phosphatase, Texas Red™ conjugated are suitable for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent Western blotting. Avidin conjugated with glucose oxidase and peroxidase are useful as a detection reagent for primary antibodies conjugated with biotin and also can be utilised in Western blotting and ELISA experiments with proper substrates.
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Atto 390 Conjugation Kit (Fast) - Lightning-Link®, Abcam
Supplier: Abcam
Labeling in < 20 mins with 30 secs hands-on time using Atto 390 Conjugation Kit (Fast) - Lightning-Link® ab269895. 100% antibody recovery.
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Streptavidin Gold Conjugate (40nm, 10 OD), Abcam
Supplier: Abcam
Labeling in < 4 hrs with 30 secs hands-on time using Streptavidin Gold Conjugate (40nm, 10 OD) ab186864. 100% antibody recovery.
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Mix-n-Stain™ Cyanine dye antibody labelling kits
Supplier: Biotium
Revolutionary technology allows antibody labelling with Cyanine 555 (Cy®3) or Cyanine 647 (Cy®5) in 30 minutes without a purification step. The labelling procedure tolerates many common buffer components and antibody stabilisers.
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