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Protein Linking and Labeling Systems

Protein Linking and Labeling Systems

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.

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Speciality dyes, DyLight™

Speciality dyes, DyLight™

Supplier: Thermo Fisher Scientific

The DyLight™ Specialty Dyes are fluorescent dyes with varying spectral characteristics, degrees of sulfonation, charges and hydrophobicities. These dyes are offered in their amine-reactive forms and are mainly derivatized with N-hydroxysuccinimide (NHS) esters, the simplest and most commonly used reactive group for labeling proteins. The large selection of near infrared (NIR) and infrared (IR) dyes may be used for in vivo and near-IR fluorescence (NIRF) applications.

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ReadiLink™ Rapid XFD555 Antibody Labelling Kit *XFD555 Same Structure to Alexa Fluor™ 555* (2×50 µg Labellings)

ReadiLink™ Rapid XFD555 Antibody Labelling Kit *XFD555 Same Structure to Alexa Fluor™ 555* (2×50 µg Labellings)

Supplier: AAT Bioquest

XFD555 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 555 (Alexa Fluor® is the trademark of ThermoFisher).

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Buccutite™ MTA-Dye 650  (2 µmoles)

Buccutite™ MTA-Dye 650 (2 µmoles)

Supplier: AAT Bioquest

Our Buccutite™crosslinking technology provides the most convenient and effective crosslinking method to link two biomolecules with a high conjugation yield.

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Cell Explorer™ Live Cell Tracking Kit

Cell Explorer™ Live Cell Tracking Kit

Supplier: AAT Bioquest

The kit uses a weakly fluorescent dye that carries a cell-retaining moiety.

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ReadiLink™ iFluor® 488 Oligo and ssDNA Labelling Kit

ReadiLink™ iFluor® 488 Oligo and ssDNA Labelling Kit

Supplier: AAT Bioquest

The kit is optimised for efficient labelling and contains all the essential reagents required for efficient labelling of ssDNA or oligos.

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ReadiLink™ Protein Biotinylation Kit *Powered by ReadiView™ Biotin Visionization Technology* (3 Reactions)

ReadiLink™ Protein Biotinylation Kit *Powered by ReadiView™ Biotin Visionization Technology* (3 Reactions)

Supplier: AAT Bioquest

Biotin is widely used for labeling biomolecules, in particular, antibodies.

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Buccutite™ Poly-HRP Antibody Conjugation Kit

Buccutite™ Poly-HRP Antibody Conjugation Kit

Supplier: AAT Bioquest

Poly-HRP-antibody conjugates is considered to have the highest sensitivity in immunoassays where the sample is limited, or a target molecule is present at extremely low level.

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Sulfo-SBED Biotin Label Transfer Kit and Reagents - Western Blot Application, Pierce™

Sulfo-SBED Biotin Label Transfer Kit and Reagents - Western Blot Application, Pierce™

Supplier: Thermo Fisher Scientific

Sulfo-SBED is a trifunctional crosslinking reagent containing a biotin, a sulfonated N-hydroxysuccinimide (Sulfo-NHS) active ester and a photoactivatable aryl azide

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Buccutite™ Rapid APC-Cy5.5 Tandem Antibody Labelling Kits (2 Labelings)

Buccutite™ Rapid APC-Cy5.5 Tandem Antibody Labelling Kits (2 Labelings)

Supplier: AAT Bioquest

APC-Cy5.5 is a popular colour used in flow cytometry.

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5-VIC Phosphoramidite

5-VIC Phosphoramidite

Supplier: AAT Bioquest

VIC phosphoramidite is the most convenient building block to incorporate VIC tag into an oligonucleotide.

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Desthiobiotinylation and pull-down kit, EZ-Link™

Desthiobiotinylation and pull-down kit, EZ-Link™

Supplier: Thermo Fisher Scientific

EZ-Link™ Desthiobiotinylation and Pull-down Kit is a complete set of reagents to biotin-label purified proteins and use them to purify interaction complexes using streptavidin agarose resin with mild elution conditions.

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Crosslink immunoprecipitation (IP) kit, Pierce™

Crosslink immunoprecipitation (IP) kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Crosslink Immunoprecipitation (IP) Kit enables highly effective and efficient antigen immunoprecipitations by covalently crosslinking antibodies onto Protein A/G resin.

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NHS ester quenchers, DyLight™

NHS ester quenchers, DyLight™

Supplier: Thermo Fisher Scientific

The DyLight™ NHS Ester Quenchers are amine-reactive non-fluorescent dyes that use fluorescent resonance energy transfer (FRET) to quench fluorescent signals resulting from ultraviolet, visible and infrared light-excited fluorescent dyes. These dyes can be efficiently paired with many DyLight™ dyes and other common fluorescent dyes including fluorescein, rhodamine, cyanine and Texas Red® dye.

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ReadiLink™ xtra Rapid iFluor® 350 Antibody Labelling Kit *BSA-Compatible* (2 Labellings)

ReadiLink™ xtra Rapid iFluor® 350 Antibody Labelling Kit *BSA-Compatible* (2 Labellings)

Supplier: AAT Bioquest

ReadiLink™ xtra rapid antibody labelling kits require essentially only two simple mixing steps without a column purification needed.

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ReadiLink™ Rapid iFluor® 350 Antibody Labelling Kit 'Microscale Optimised for Labelling 50 µg Antibody Per Reaction' (2 Labellings)

ReadiLink™ Rapid iFluor® 350 Antibody Labelling Kit 'Microscale Optimised for Labelling 50 µg Antibody Per Reaction' (2 Labellings)

Supplier: AAT Bioquest

AAT Bioquest's iFluor® dyes are optimised for labelling proteins, in particular, antibodies.

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ReadiLink™ DIG (Digoxigenin) Nick Translation dsDNA Labeling Kit

ReadiLink™ DIG (Digoxigenin) Nick Translation dsDNA Labeling Kit

Supplier: AAT Bioquest

It is compatible with a wide variety of sample materials, including bacterial artificial chromosome (BAC) DNA, human genomic DNA, purified PCR products, supercoiled and linearized plasmid DNA.

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