Protein Linking and Labeling Systems
Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.
ReadiLink™ xtra Rapid Cy3 Antibody Labeling Kit *BSA-Compatible* (2 Labelings)
Supplier: AAT Bioquest
ReadiLink™ xtra rapid antibody labelling kits require essentially only 2 simple mixing steps without a column purification needed.
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Activated Horseradish Peroxidase (HRP), EZ-Link™ Plus
Supplier: Thermo Fisher Scientific
The Thermo Scientific™ EZ-Link™ Plus Activated Peroxidase is an amine-reactive horseradish peroxidase (HRP) that provides higher conjugate yields (>95%) than glutaraldehyde chemistry.
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iFluor® 647-Dextran Conjugate
Supplier: AAT Bioquest
iFluor® 647-dextran (70 kDa) is a large far-red dextran conjugate that selectively labels blood vessels and extracellular spaces, enabling high-resolution imaging of vascular dynamics and permeability.
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Wheat Germ Agglutinin, XFD555 Labelled *XFD555 Same Structure to Alexa Fluor® 555*
Supplier: AAT Bioquest
XFD555, manufactured by AAT Bioquest, has an identical chemical structure to Alexa Fluor® 555, a trademark of ThermoFisher.
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Amine reactive biotinylation reagents, EZ-Link™
Supplier: Thermo Fisher Scientific
The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.
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Amine reactive biotinylation kits, EZ-Link™
Supplier: Thermo Fisher Scientific
The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.
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ReadiLink™ Rapid iFluor® Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)
Supplier: AAT Bioquest
Although FITC is still the most popular fluorescent labelling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence.
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ReadiLink™ xtra Rapid FITC Antibody Labelling Kit *BSA-Compatible* (2 Labellings)
Supplier: AAT Bioquest
ReadiLink™ xtra rapid antibody labelling kits require essentially only 2 simple mixing steps without a column purification needed.
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Buccutite™ Rapid APC-Cy7 Tandem Antibody Labelling Kit
Supplier: AAT Bioquest
Production scale optimised for labelling 1 mg antibody per reaction. Buccutite™ rapid APC-Cy7 tandem antibody labelling kits, designed for large-scale production, provide a streamlined approach for labelling antibodies with PE, APC, PerCP and iFluor® tandem dyes.
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Dyes, DyLight™
Supplier: Thermo Fisher Scientific
DyLight™ Dyes have absorption spectra ranging from 350 nm to 770 nm and match the output wavelengths of common fluorescence instrumentation.
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Annexin V, FITC Labelled
Supplier: AAT Bioquest
Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium.
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Wheat Germ Agglutinin, XFD750 Labelled *XFD750 Same Structure to Alexa Fluor® 750*
Supplier: AAT Bioquest
XFD750, manufactured by AAT Bioquest, has an identical chemical structure to Alexa Fluor® 750, a trademark of ThermoFisher.
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ReadiLink™ Rapid XFD647 Antibody Labelling Kit *XFD647 Same Structure to Alexa Fluor™ 647* (2×50 µg Labellings)
Supplier: AAT Bioquest
XFD647 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 647 (Alexa Fluor® is the trademark of ThermoFisher).
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PSA™ iFluor® 647 Imaging Kit with Goat Anti-Rabbit IgG
Supplier: AAT Bioquest
iFluor® 647 PSA kit is a much superior replacement for Alexa Fluor 647 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.
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ReadiLink™ Biotin Oligo and ssDNA Labelling Kit
Supplier: AAT Bioquest
The kit is optimised for efficient labelling and contains all the essential reagents required for efficient labelling of ssDNA or oligos.
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ReadiLink™ iFluor® 488 FISH Fluorescence Imaging Kit
Supplier: AAT Bioquest
The kit provides Taq DNA polymerase enzyme, which incorporates iFluor® 488-dUTPs in the target DNA through Polymerase Chain Reaction (PCR).
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