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Sistemi di associazione e marcatura delle proteine

Sistemi di associazione e marcatura delle proteine

I sistemi di coniugazione e marcatura delle proteine offrono un mezzo pratico per collegare marcatori fluorescenti o biotine ad anticorpi e proteine più grandi. Questi kit sono ideali per molte applicazioni, tra cui citometria a flusso, microscopia a fluorescenza, immunoistochimica, rilevamento primario, ELISA, immunocitochimica e FISH. Questi sistemi sono disponibili con una varietà di coloranti, biotina, agenti fluorescenti e tutti i componenti necessari per eseguire più coniugazioni e purificazioni. La marcatura delle proteine segue un protocollo semplice: reazione, separazione e quindi utilizzo.

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Coelenterazine Substrates for Renilla Luciferase and Apoaequorin

Supplier: Biotium

Coelenterazine and its analogs are luminescent enzyme substrates for apoaequorin and Renilla luciferase.

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CF™ dye streptavidin and monoclonal mouse anti-biotin conjugates

Supplier: Biotium

CF™ dyes are superior to other fluorescent dyes for protein labelling due to advantages in brightness, photostability and water solubility.

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PE / R-Phycoerythrin Conjugation Kit - Lightning-Link®, Abcam

Supplier: Abcam

The Abcam PE/R-Phycoerythrin Conjugation Kit (ab102918) offers several standout features: -Rapid Conjugation: achieve PE/R-Phycoerythrin labeling in under 4 hours with just 30 seconds of hands-on time. -High Efficiency: ensures 100% antibody recovery, preserving the integrity and value of your antibodies. -Versatility: suitable for conjugating antibodies, proteins, and peptides, and the labeled antibodies can be used immediately in applications such as Western Blotting (WB), ELISA, and Immunohistochemistry (IHC) without further purification. - Confidence: cited in over 50 publications

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Cy3® Conjugation Kit (Fast) - Lightning-Link®, Abcam

Supplier: Abcam

Cy3® conjugation / labeling in < 20 mins with 30 secs hands-on time using Cy3® Conjugation Kit (Fast) - Lightning-Link® ab188287. 100% antibody recovery.

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DyLight® 650 Conjugation Kit (Fast) - Lightning-Link®, Abcam

Supplier: Abcam

DyLight® 650 conjugation / labeling in < 20 mins with 30 secs hands-on time using DyLight® 650 Conjugation Kit (Fast) - Lightning-Link® ab201803. 100% antibody recovery.

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Antibody labelling kits, Mix-n-Stain™ FITC

Antibody labelling kits, Mix-n-Stain™ FITC

Supplier: Biotium

Mix-n-Stain™ FITC antibody labelling kits utilise a technology that allows rapid antibody labelling with green fluorescent dye (FITC) without a purification step.

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HRP-conjugate stabiliser, SeramunStab® STA2

Supplier: Apollo Scientific

Liquid product red odourless pH-value 7,3 to 7,5 Boiling point 102 °C. Stabiliser for a broad range of HRP conjugates.

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XFD750 Conjugation Kit, Abcam

Supplier: Abcam

XFD750 conjugation / labeling in < 4 hrs with 30 secs hands-on time using XFD750 Conjugation Kit ab312814. 100% antibody recovery.

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Maleimide-Alkaline Phosphatase, Abcam

Supplier: Abcam

Maleimide-Alkaline Phosphatase. Achieve your results faster with highly validated, pure and trusted compounds.

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165Ho Metal Conjugation Kit - Lightning-Link®, Abcam

Supplier: Abcam

Labeling in < 4 hrs with 30 secs hands-on time using 165Ho Metal Conjugation Kit - Lightning-Link® ab269844. 100% antibody recovery.

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DoubleStain IHC Kit: R&Rt on human/mouse tissue (Green/HRP & AP/Red), Abcam

Supplier: Abcam

Abcam's Doublestain IHC Kit (ab183285) is designed for use with user supplied rabbit and rat primary antibodies to detect two distinct antigens on human and mouse tissue or cell samples.

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VeriBlot for IP Detection Reagent (HRP)

Supplier: Abcam

VeriBlot for IP detection reagent (HRP) is an immunoblotting reagent that enables the trouble-free detection of immunoblotted target proteins without interference from denatured IgG. - Cited in over 80 publications - Detects target proteins without interference from IgG heavy and light chains - Recognizes non-reduced antibodies, minimizing detection of denatured IgG - Multiple species compatibility – detects IgG from bovine, goat, human, mouse, rat, rabbit, and sheep (specific isotypes detailed) - Suitable for up to 20 blots with proper dilution (1:200 in 5 ml milk)

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CF® Dye cAMP

Supplier: Biotium

Fluorescently labelled cAMP analogs that can be used to probe cAMP receptors.

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rProtein A/G Beads 4FF, Abcam

Supplier: Abcam

Supplemental Information * Ligand: Recombinant Protein A/G * Basic Beads: 4% Cross-linked agarose beads * Particle size: 45μm – 165μm * Storage Buffer: 80% PBS, 20% ethanol * pH Stability Range: 3-10 * Maximum pressure: 0.3 MPa * Binding Capacity: 10-15 mg Rabbit lgG/ml .

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ExoBrite™ True EV Membrane Stains

Supplier: Biotium

Lipophilic membrane dyes designed specifically for EV research and the best choice for general pan-EV staining.

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Alexa Fluor® 700 Conjugation Kit (Fast) - Lightning-Link®, Abcam

Supplier: Abcam

Alexa Fluor® 700 conjugation / labeling in < 20 mins with 30 secs hands-on time using Alexa Fluor® 700 Conjugation Kit (Fast) - Lightning-Link® ab269824. 100% antibody recovery.

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