Protein Linking and Labeling Systems
Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.
Carboxyl reactive biotin reagents, HOOK™
Supplier: G-Biosciences
HOOK™ Biotin-PEG₂-Amine, and its long chain form, HOOK™ Biotin-PEG₃-Amine, are carboxyl reactive biotinylation reagents. These agents contain terminal amines and react with carboxyl groups found at the carboxyl termini, aspartate, and glutamate side chains. The reaction is mediated by a water-soluble carbodiimide (EDC). The carbodiimide activates the carboxyl group and reacts with the amines (-NH₂) on the biotinylation agent to form an amide bond. This reaction is rapid and takes just a few minutes to complete. Under these conditions, hydrazide-derivatives of biotin reagents may also react with the carboxyls.
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iFluor® 350-Wheat Germ Agglutinin (WGA) Conjugate
Supplier: AAT Bioquest
iFluor® 350 WGA conjugate, like Alexa Fluor® 350 WGA conjugate, retains its ability to bind to sialic acid and N-acetylglucosaminyl residues, enhancing its utility in fluorescence imaging and analysis for various scientific investigations.
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DY647 CEP (DyLight 647 phosphoramidite)
Supplier: AAT Bioquest
DY647 CEP (DyLight 647 phosphoramidite) is an excellent replacement to the commonly used Cy5 phosphoramidite.
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Fluor phosphine reagents, DyLight™, Pierce™
Supplier: Thermo Fisher Scientific
DyLight™ Fluor phosphine reagents are phosphine-activated fluorescent dyes for specific labeling and detection of azide-tagged molecules, which enables use of fluorescence imaging in metabolic labeling strategies.
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ReadiLink™ Rapid iFluor® 700 Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)
Supplier: AAT Bioquest
AAT Bioquest's iFluor® dyes are developed for labelling proteins, in particular, antibodies.
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PSA™ iFluor® 488 Imaging Kit with Goat Anti-Mouse IgG
Supplier: AAT Bioquest
iFluor® 488 PSA kit is a much superior replacement for Alexa Fluor 488 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.
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Buccutite™ Rapid PerCP Antibody Labelling Kits (2 Labellings)
Supplier: AAT Bioquest
PerCP (Peridinin-chlorophyll-protein complex) is isolated from Dinophyceae sp.
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HIS Lite™ iFluor® 647 Tris NTA Chelator
Supplier: AAT Bioquest
iFluor® 647 Tris-NTA Chelator is used as a sensitive red fluorescent probe for detecting polyhistidine-labelled proteins in combination with the addition of a heavy metal ion such as Ni²⁺ in cells, solution and solid surfaces.
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Carbohydrate reactive biotinylation reagents, EZ-Link™
Supplier: Thermo Fisher Scientific
A common target for protein modification is the carbohydrate portion of glycoproteins, which can be reacted with hydrazide derivatives of biotin. Oxidative treatment of glycoproteins using 10 mM periodate is used to generate reactive aldehydes from the cis-diols of a variety of carbohydrate moieties.
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ReadiLink™ Rapid iFluor® 633 Antibody Labelling Kit 'Microscale Optimised for Labelling 50 µg Antibody Per Reaction' (2 Labellings)
Supplier: AAT Bioquest
iFluor® dyes are developed for labelling proteins, in particular, antibodies.
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ReadiLink™ Rapid iFluor® 594 Antibody Labelling Kit
Supplier: AAT Bioquest
ReadiLink™ Rapid antibody labelling kits, designed for production scale, provide a convenient and efficient method for labelling large volumes of antibodies with our superior iFluor® dyes.
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ReadiLink™ xtra Rapid iFluor® 594 Antibody Labeling Kit *BSA-Compatible* (2 Labelings)
Supplier: AAT Bioquest
ReadiLink™ xtra rapid antibody labelling kits require essentially only 2 simple mixing steps without a column purification needed.
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HIS Lite™ Cy3 Tris NTA Chelator
Supplier: AAT Bioquest
Cy3-Tris NTA compound is a sensitive fluorescent chelator for detecting polyhistidine-labelled proteins in cells, solution, and solid surfaces with the addition of certain metal ions (such as Ni²⁺, Co₂⁺ and more) as a mediator.
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ReadiLink™ Rapid iFluor® 594 Antibody Labelling Kit 'Microscale Optimised for Labelling 50 µg Antibody Per Reaction' (2 Labellings)
Supplier: AAT Bioquest
AAT Bioquest's iFluor® dyes are optimised for labelling proteins, in particular, antibodies.
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PSA™ iFluor® 350 Imaging Kit with Goat Anti-Rabbit IgG
Supplier: AAT Bioquest
iFluor® 350 PSA kit is a much superior replacement for Alexa Fluor 350 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.
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HIS Lite™ Cy5 Tris NTA-Ni Complex
Supplier: AAT Bioquest
Cy5-Tris NTA compound is used as a sensitive fluorescent probe for detecting polyhistidine-labelled proteins in cells, solution and solid surfaces.
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