Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Research buffers and reagents
Supplier: G-Biosciences
These DNase- and RNase-free buffers and reagents are designed for high quality research.
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Blocking buffers, Protein-Free™
Supplier: G-Biosciences
Protein-Free™ Blocking buffer is a formulation of non-protein agents that eliminate non-specific binding sites in ELISA, blotting, immunohistochemistry and other applications. The absence of protein from the Protein-Free™ Blocking buffer eliminates problems associated with traditional protein based blockers, such as cross-reactivity and interference from glycosylated proteins.
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VWR® Buffer, MOPS-SDS, 20X, UltraPure Grade
Supplier: VWR Chemicals
Useful for high resolution of proteins on neutral pH SDS-PAGE gels.
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VWR®, Blocking buffer, non-fat powdered milk
Supplier: VWR Chemicals
Each pack of powdered blocking buffer makes one liter of 1X solution.
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Buffer, HEPES solution (1 mol/L)
Supplier: Cayman Chemical
HEPES buffer solution (1 mol/l)
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Phosphate buffered saline (PBS), 10X
Supplier: G-Biosciences
PBS 10X solution is used as a wash buffers for Western blotting, ELISA and other applications.
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VWR® A.C.E.™, Sequencing buffers
Supplier: VWR Chemicals
A.C.E.™ Sequencing Buffers are optimised buffers for DNA capillary electrophoresis sequencing that provide excellent results like long read lengths, high resolution, and consistent run times.
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Phosphate Buffer Solution
Supplier: SIGMA ALDRICH MICROSCOPY
Contains sodium and potassium phosphates. Intended for use in the Sigma Butyrate Esterase Procedure, No. 181B.
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Buffer, EDTA buffer solution pH 8,0 (0,5 mol/L)
Supplier: Rockland Immunochemicals
EDTA is widely used for scavenging metal ion in biochemistry and molecular biology. Ion depletion is commonly used to deactivate metal-dependent enzymes, either as an assay for their reactivity or to suppress damage to DNA or proteins.
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Buffer, PBS (10X, Dulbecco's) powder pack
Supplier: PanReac AppliChem
Powder mixture to prepare PBS buffer (10X) according to Dulbecco.
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Permeabilisation buffer
Supplier: Biotium
Ready-to-use permeabilisation buffer for intracellular immunofluorescence staining for microscopy or flow cytometry.
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Instant Buffers (acc. to Soerensen)
Supplier: TCI
Instant Buffer S64, (acc. to Soerensen), pH value: 6.4
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VWR® Proteomics Grade, Tris-glycine buffer
Supplier: VWR Chemicals
A pre-blended Tris-glycine powder for preparation of Laemmli SDS-PAGE running buffer. The 40 litre pack size prepares 40 litres of 1X Tris-glycine solution (0,025 M Tris base and 0,192 M glycine). Each Ready-Pack™ prepares 1 litre of a 10X concentrate.
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Cesium nitrate, ionization buffer solution, Specpure®
Supplier: Thermo Fisher Scientific
Cesium Nitrate Buffer solution
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VWR® Lysis Buffer, Hypotonic
Supplier: VWR Chemicals
Detergent-free buffer for isolation of proteins from tissue culture cells. Cell lysis and the subsequent isolation of the crude cytoplasmic fraction completed in less than 30 m.
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Trizmal™ Buffer, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
For use in the Naphthol AS-D Chloroacetate Esterase Kit No. 91-C.
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VWR® Buffer, HEPES Solution pH 7,3 (1 mol/L)
Supplier: VWR Chemicals
HEPES buffer solution pH 7.3 (1 mol/l), VWR®
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Buffer, SSC solution 20X concentrate (saline-sodium citrate)
Supplier: G-Biosciences
Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)
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Lithium nitrate, ionisation buffer solution, Specpure®
Supplier: Thermo Fisher Scientific
Lithium Nitrate Buffer Solution
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VWR® Buffer, MES-SDS, 20X, UltraPure Grade
Supplier: VWR Chemicals
Useful for high resolution of proteins on neutral pH SDS-PAGE gels.
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Buffer, SSC solution 20X concentrate (saline-sodium citrate)
Supplier: G-Biosciences
Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)
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WB blocking buffer kit, TrueBlack®
Supplier: Biotium
The TrueBlack® WB blocking buffer kit is a ready-to-use buffer system that provides optimal specificity, sensitivity, and background signal suppression for fluorescence-based western blotting (WB).
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Buffer, EDTA buffer solution pH 8,5 (0,5 mol/L)
Supplier: G-Biosciences
A DNase and RNase free solution of 0.5M EDTA.
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SDS-PAGE Sample Buffer, 2X, (2-Mercaptoethanol-free) for Electrophoresis
Supplier: TCI
SDS-PAGE Sample Buffer is specially formulated for protein sample preparation to based on the Laemmli Method of SDS-PAGE system.
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Phosphate buffer solution, BioUltra, Supelco®
Supplier: Merck
Phosphate buffer, BioUltra, >0,044 M Na₂HPO₄, >0,022 M NaH₂PO₄, pH value: 7,2
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Buffer HF-Improved
Supplier: Thermo Fisher Scientific
Buffer used for pretreatment of planar silicon devices when plating with nickel plating solution products 44069 and 44070.
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VWR® Tris Buffer Solution, pH 7,5 (1 mol/L), UltraPure Grade
Supplier: VWR Chemicals
TRIS buffer solution pH 7,5 (1 mol/l), VWR®
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VWR® Sodium Acetate Buffer Solution, pH 5,2 (3 mol/L) for Biotechnology
Supplier: VWR Chemicals
Sodium acetate buffer, VWR®, 3 M, pH value: 5,2
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VWR® Phosphate Buffered Saline (PBS), High Phosphate
Supplier: VWR Chemicals
Irradiated solution, pH 7,2, for washing blots.
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GET buffer
Supplier: Rockland Immunochemicals
GET buffer (50 mM Glucose, 10 mM EDTA, 25 mM Tris HCl, pH 8,0) is used in effective plasmid miniprep.