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Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

Research buffers and reagents

Research buffers and reagents

Supplier: G-Biosciences

These DNase- and RNase-free buffers and reagents are designed for high quality research.

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Blocking buffers, Protein-Free™

Supplier: G-Biosciences

Protein-Free™ Blocking buffer is a formulation of non-protein agents that eliminate non-specific binding sites in ELISA, blotting, immunohistochemistry and other applications. The absence of protein from the Protein-Free™ Blocking buffer eliminates problems associated with traditional protein based blockers, such as cross-reactivity and interference from glycosylated proteins.

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VWR® Buffer, MOPS-SDS, 20X, UltraPure Grade

VWR® Buffer, MOPS-SDS, 20X, UltraPure Grade

Supplier: VWR Chemicals

Useful for high resolution of proteins on neutral pH SDS-PAGE gels.

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VWR®, Blocking buffer, non-fat powdered milk

VWR®, Blocking buffer, non-fat powdered milk

Supplier: VWR Chemicals

Each pack of powdered blocking buffer makes one liter of 1X solution.

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Buffer, HEPES solution (1 mol/L)

Supplier: Cayman Chemical

HEPES buffer solution (1 mol/l)

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Phosphate buffered saline (PBS), 10X

Phosphate buffered saline (PBS), 10X

Supplier: G-Biosciences

PBS 10X solution is used as a wash buffers for Western blotting, ELISA and other applications.

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VWR® A.C.E.™, Sequencing buffers

VWR® A.C.E.™, Sequencing buffers

Supplier: VWR Chemicals

A.C.E.™ Sequencing Buffers are optimised buffers for DNA capillary electrophoresis sequencing that provide excellent results like long read lengths, high resolution, and consistent run times.

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Phosphate Buffer Solution

Supplier: SIGMA ALDRICH MICROSCOPY

Contains sodium and potassium phosphates. Intended for use in the Sigma Butyrate Esterase Procedure, No. 181B.

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Buffer, EDTA buffer solution pH 8,0 (0,5 mol/L)

Buffer, EDTA buffer solution pH 8,0 (0,5 mol/L)

Supplier: Rockland Immunochemicals

EDTA is widely used for scavenging metal ion in biochemistry and molecular biology. Ion depletion is commonly used to deactivate metal-dependent enzymes, either as an assay for their reactivity or to suppress damage to DNA or proteins.

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Buffer, PBS (10X, Dulbecco's) powder pack

Buffer, PBS (10X, Dulbecco's) powder pack

Supplier: PanReac AppliChem

Powder mixture to prepare PBS buffer (10X) according to Dulbecco.

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Permeabilisation buffer

Permeabilisation buffer

Supplier: Biotium

Ready-to-use permeabilisation buffer for intracellular immunofluorescence staining for microscopy or flow cytometry.

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Instant Buffers (acc. to Soerensen)

Supplier: TCI

Instant Buffer S64, (acc. to Soerensen), pH value: 6.4

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VWR® Proteomics Grade, Tris-glycine buffer

VWR® Proteomics Grade, Tris-glycine buffer

Supplier: VWR Chemicals

A pre-blended Tris-glycine powder for preparation of Laemmli SDS-PAGE running buffer. The 40 litre pack size prepares 40 litres of 1X Tris-glycine solution (0,025 M Tris base and 0,192 M glycine). Each Ready-Pack™ prepares 1 litre of a 10X concentrate.

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Cesium nitrate, ionization buffer solution, Specpure®

Supplier: Thermo Fisher Scientific

Cesium Nitrate Buffer solution

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VWR® Lysis Buffer, Hypotonic

VWR® Lysis Buffer, Hypotonic

Supplier: VWR Chemicals

Detergent-free buffer for isolation of proteins from tissue culture cells. Cell lysis and the subsequent isolation of the crude cytoplasmic fraction completed in less than 30 m.

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Trizmal™ Buffer, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

For use in the Naphthol AS-D Chloroacetate Esterase Kit No. 91-C.

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VWR® Buffer, HEPES Solution pH 7,3 (1 mol/L)

VWR® Buffer, HEPES Solution pH 7,3 (1 mol/L)

Supplier: VWR Chemicals

HEPES buffer solution pH 7.3 (1 mol/l), VWR®

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Buffer, SSC solution 20X concentrate (saline-sodium citrate)

Supplier: G-Biosciences

Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)

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Lithium nitrate, ionisation buffer solution, Specpure®

Supplier: Thermo Fisher Scientific

Lithium Nitrate Buffer Solution

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VWR® Buffer, MES-SDS, 20X, UltraPure Grade

VWR® Buffer, MES-SDS, 20X, UltraPure Grade

Supplier: VWR Chemicals

Useful for high resolution of proteins on neutral pH SDS-PAGE gels.

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Buffer, SSC solution 20X concentrate (saline-sodium citrate)

Supplier: G-Biosciences

Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)

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WB blocking buffer kit, TrueBlack®

Supplier: Biotium

The TrueBlack® WB blocking buffer kit is a ready-to-use buffer system that provides optimal specificity, sensitivity, and background signal suppression for fluorescence-based western blotting (WB).

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Buffer, EDTA buffer solution pH 8,5 (0,5 mol/L)

Supplier: G-Biosciences

A DNase and RNase free solution of 0.5M EDTA.

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SDS-PAGE Sample Buffer, 2X, (2-Mercaptoethanol-free) for Electrophoresis

Supplier: TCI

SDS-PAGE Sample Buffer is specially formulated for protein sample preparation to based on the Laemmli Method of SDS-PAGE system.

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Phosphate buffer solution, BioUltra, Supelco®

Supplier: Merck

Phosphate buffer, BioUltra, >0,044 M Na₂HPO₄, >0,022 M NaH₂PO₄, pH value: 7,2

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Buffer HF-Improved

Supplier: Thermo Fisher Scientific

Buffer used for pretreatment of planar silicon devices when plating with nickel plating solution products 44069 and 44070.

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VWR® Tris Buffer Solution, pH 7,5 (1 mol/L), UltraPure Grade

VWR® Tris Buffer Solution, pH 7,5 (1 mol/L), UltraPure Grade

Supplier: VWR Chemicals

TRIS buffer solution pH 7,5 (1 mol/l), VWR®

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VWR® Sodium Acetate Buffer Solution, pH 5,2 (3 mol/L) for Biotechnology

VWR® Sodium Acetate Buffer Solution, pH 5,2 (3 mol/L) for Biotechnology

Supplier: VWR Chemicals

Sodium acetate buffer, VWR®, 3 M, pH value: 5,2

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VWR® Phosphate Buffered Saline (PBS), High Phosphate

VWR® Phosphate Buffered Saline (PBS), High Phosphate

Supplier: VWR Chemicals

Irradiated solution, pH 7,2, for washing blots.

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GET buffer

Supplier: Rockland Immunochemicals

GET buffer (50 mM Glucose, 10 mM EDTA, 25 mM Tris HCl, pH 8,0) is used in effective plasmid miniprep.

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