Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Electrophoresis running buffers
Supplier: G-Biosciences
There are many electrophoresis running buffers including: TAE running buffer (50×), TBE running buffer (10×), TBE-urea sample buffer, MOPS running buffer (10×), and MOPS/ SDS running buffer (20×). These include the SDS-PAGE sample loading buffer, SDS-PAGE running buffer, native sample loading buffer, tris glycine native gel running buffer, tricine sample buffer, tris-tricine, tris-tricine-SDS, MES SDS running buffer, MOPS/ SDS running buffer, IEF anode buffer, IEF cathode buffer, zymogram sample buffer, zymogram renature buffer, zymogram development buffer and the discontinuous buffer system.
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Dry buffer packs, Carbonate-Bicarbonate Buffer, JAW™
Supplier: G-Biosciences
Premixed buffer powders that simply require adding deionised water to generate a ready-to-use buffer.
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Blocking buffers, FirstChoice™
Supplier: G-Biosciences
FirstChoice™ Blocking Buffer is ideal as a first choice for optimization of new assays and systems, or when determining the optimal blocking buffer for elimination of non specific binding sites in ELISA, blotting, immunohistochemistry and other applications. Buffers are conveniently supplied in widely used TBS (Tris-buffered saline at pH 7,5) and PBS (phosphate-buffered saline at pH 7,5) buffers as well as in separate formulations containing Tween® 20 for improving blocking efficiencies.
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G-Biosciences TBE Running Buffer (10X)
Supplier: G-Biosciences
The primary use of TBE running buffer is in agarose electrophoresis for the separation of nucleic acids (DNA and RNA).
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Gram Negative lysis buffer
Supplier: G-Biosciences
Gram-negative lysis buffer is an extraction buffer for soluble proteins from Gram-negative bacteria. It is a proprietary improvement on the lysozyme based lysis in combination with various salts and agents, which allows extraction of soluble proteins. This buffer has been tested for use with several widely used bacteria including E.coli strains.
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G-Biosciences TBE-Urea Sample Buffer
Supplier: G-Biosciences
Loading buffer to resolve single-stranded DNA oligos or RNA into sharp, distinct bands.
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Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)
Supplier: PanReac AppliChem
Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)
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Phosphate buffered saline, PBS-TWEEN® tablets, Millipore®
Supplier: Merck Millipore (Oncogene)
Developed for use in immunoassay applications.
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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)
Supplier: Rockland Immunochemicals
Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)
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Buffer, TBE solution 5X concentrate (TRIS-borate-EDTA buffer)
Supplier: Biotium
Running Buffer, TBE buffer solution 5X concentrate (TRIS-borate-EDTA buffer)
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SDS-Page running gel buffers, 10X concentrate
Supplier: Rockland Immunochemicals
SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature. SDS is an anionic detergent applied to protein sample to linearise proteins and to impart a negative charge to linearised proteins.
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Proteometer-CV mobile phase A salts
Supplier: NOVILYTIC
5 sealed salt packets specifically formulated for use with Proteometer-CV kit.
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Protein blocking buffers, Superior™
Supplier: G-Biosciences
Blocking buffer is an enhanced blocking agent available in multiple formats. Superior™ Blocking Buffer contains a proprietary antigenically non-determinant protein for blocking Western blot membranes, blocking and storage of ELISA plates, and blocking prior to immunohistochemistry staining.
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G-Biosciences Sodium Chloride-Sodium Citrate Buffer (20X)
Supplier: G-Biosciences
Saline-Sodium Citrate Hybridization Buffer (20X) controls stringency during the washing steps in Southern blotting, in situ hybridization, DNA microarray or Northern blotting.
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G-Biosciences Hybridization Buffer (Hybridization Neutralizing Solution)
Supplier: G-Biosciences
Ready-to-Use: Convenient, time-saving formulation ideal for consistent experimental reproducibility.
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G-Biosciences Hybridization Buffer (Hybridization Denaturing Solution)
Supplier: G-Biosciences
Ready-to-Use: Convenient, time-saving formulation ideal for consistent experimental reproducibility.
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