Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

Buffer, TBS solution 10X concentrate (TRIS buffered saline) pH 7,4

Supplier: Cayman Chemical

Formulation: Concentrated TBS (250 mM Tris-base; pH 7,4; 1,37 M sodium chloride and 27 mM KCl).

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PBS and TBS buffers

Supplier: Rockland Immunochemicals

Phosphate buffered and Tris buffered saline are suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Also may be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA. Other applications may require detergents or other additional components.

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Kinase Dilution Buffer III, Abcam

Supplier: Abcam

Solution of buffer used for diluting kinase protein.

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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L)

Supplier: Thermo Fisher Scientific

Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)

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Blocking buffer, fish gelatin

Supplier: Biotium

Fish gelatin is commonly used in blocking solutions to minimise background noise in immunodetection procedures such as Western Blotting and immunostaining. Fish gelatin can be used to block non-specific binding sites on positively charged nylon or PVDF membranes or for blocking and dilution of antibodies during immunostaining procedures. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies.

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Buffers, TRIS HCl solutions, pH range 7,2 to 8,5 (1 mol/L)

Supplier: Rockland Immunochemicals

Tris is widely used as a component of buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids.

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TPE buffer, 10X tris-phosphate-EDTA, pH 8

Supplier: Rockland Immunochemicals

Running Buffer, Tris-phosphate-EDTA 10X (TPE) (0,9 M tris-phosphate 0,01 M EDTA pH 8,0)

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Blocking buffers

Supplier: Rockland Immunochemicals

A blocking buffer specifically designed for western blotting using fluorochrome conjugated antibodies. Blocking buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in tris buffered saline at pH 7,6 with thimerisol added as an antimicrobial agent.

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Native lysis Buffer, Abcam

Supplier: Abcam

Abcam’s 1X Native lysis buffer is an efficient means of cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells.

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100x Citrate Buffer pH 6.0, Abcam

Supplier: Abcam

Citrate buffer pH 6.0 for heat-induced antigen retrieval (HIER) during IHC (ab64236).

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Dry buffer packs, JAW™

Supplier: G-Biosciences

Premixed buffer powders that simply require adding water to generate a ready-to-use buffer.

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SDS-PAGE Sample Buffer, 2X, (2-Mercaptoethanol-free) for Electrophoresis

Supplier: TCI

SDS-PAGE Sample Buffer is specially formulated for protein sample preparation to based on the Laemmli Method of SDS-PAGE system.

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Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)

Supplier: PanReac AppliChem

Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)

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Phosphate buffered saline, PBS-TWEEN® tablets, Millipore®

Supplier: Merck Millipore (Oncogene)

Developed for use in immunoassay applications.

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)

Supplier: Rockland Immunochemicals

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

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Extraction Buffer 5X PTR, Abcam

Supplier: Abcam

Extraction buffer 5X PTR is formulated for preparing cell or tissue extracts for use in immunoassays, with added phosphatase inhibitors and protease inhibitor aprotinin. - Cited in over 20 publications - Phosphatase and protease inhibitors – contains inhibitors to preserve sample integrity - Easy to use – dilute to 1X with distilled water before use, or use with extraction enhancer buffer 50X for enhanced extraction - Compatible with a variety of sample types