Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

VWR® A.C.E.™, Sequencing buffers

Supplier: VWR Chemicals

A.C.E.™ Sequencing Buffers are optimised buffers for DNA capillary electrophoresis sequencing that provide excellent results like long read lengths, high resolution, and consistent run times.

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VWR®, SSC buffer, 20X, ultra-pure grade

Supplier: VWR Chemicals

SSC buffer is a hybridisation buffer for Northern and Southern blots.

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VWR® Separating Gel Buffer, 4X

Supplier: VWR Chemicals

4X concentrated buffer solution (1,5 M Tris, pH 8,8 and 0,4% SDS). Used for preparation of standard acrylamide:bis-acrylamide gels as well as preparation of gels using PAGE-PLUS™ acrylamide solution.

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VWR®, Eluent buffers for LC

Supplier: VWR Chemicals

LC eluent A, LUFA

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VWR® Buffer, Ammonium Chloride, pH 10, Reag. Ph.Eur.

Supplier: VWR Chemicals

The reagent is specially intended for use in testing Ph Eur and monographs. This solution was produced according 4.1.1 of Ph Eur specifications using Ph Eur reagent quality components and water meeting the Ph Eur monograph requirement for purified water.

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J.T.Baker® DILUT-IT™, Potassium Phosphate, Dissolution Media Concentrate

Supplier: Avantor

DILUT-IT is a ready-to-use dissolution media concentrate, which is pre-measured and only need to be diluted with purified water.

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VWR® PBS/Tween® 20, 20X, Blot Wash Buffer, UltraPure Grade

Supplier: VWR Chemicals

PBS Tween® 20 blot wash buffer is a 20X concentration with 1% Tween® 20.

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VWR®, Phosphate buffered saline (PBS) 1X, for biotechnology

Supplier: VWR Chemicals

137 mM sodium chloride, 2,7 mM potassium chloride, 12 mM phosphate buffer

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VWR®RapidBlock™, Blocking solution, 10x

Supplier: VWR Chemicals

RapidBlock™ solution reduces blocking time to five minutes for Western and dot blotting procedures on PVDF and nitrocellulose membranes. This protein-free formulation minimises cross-reactivity and non specific antibody binding, generating blots with low background and enhanced signal-to-noise ratio.

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VWR® Tris Buffer Solution, pH 7,4 (0,1 mol/L) for Biotechnology

Supplier: VWR Chemicals

Cell culture buffer, TRIS buffer solution pH 7,4 (0,1 mol/l)

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J.T.Baker® BAKER ANALYZED®, Buffer Solutions, Colour Coded

Supplier: Avantor

The quality of many BAKER ANALYZED™ reagents meets or exceeds the requirements set forth by the American Chemical Society (ACS).

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VWR®, Buffers, ammonia- ammonium chloride and ammonium chloride-ammonium hydroxide USP Test solutions (TS)

Supplier: VWR Chemicals

Ready to use test solutions (TS).

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VWR®, Rapid Transfer buffer 10X

Supplier: VWR Chemicals

Rapid transfer buffer is a simple, single component system for quick and efficient transfer of proteins from SDS-PAGE gels to membranes for Western blotting applications. Transfer is completed in 10 or 20 minutes using a standard semi-dry or wet transfer apparatus, respectively.