Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
TRIS-buffered saline (TBS, 10X) pH 7,4, for Western blot
Supplier: Thermo Fisher Scientific
Washing buffer, TRIS-buffered saline (TBS, 10X)
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20X SSPE, liquid, Corning®
Supplier: Corning
20X Saline-Sodium-Phosphate-EDTA (SSPE) buffer has applications in nucleic acid hybridization and other molecular biology work.
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TEA, 0,2 M buffer soln., pH 8,0
Supplier: Thermo Fisher Scientific
TEA buffer solution, 0,2 M, pH value: 8,0, Colourless, Odourless, Liquid
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TRIS-glycine-SDS running buffer (10X), pH 8,3 test
Supplier: Thermo Fisher Scientific
Running Buffer, TRIS-glycine-SDS running buffer (10X)
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TRIS-buffered saline (TBS, 10X) pH 7,6
Supplier: Thermo Fisher Scientific
Washing buffer, TRIS-buffered saline (TBS, 10X)
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HEPES-buffered saline, pH 7,0 (2X for transfection)
Supplier: Thermo Fisher Scientific
Cell culture buffer, HEPES-buffered Saline
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TRIS-glycine native sample buffer (4X), pH 8,6
Supplier: Thermo Fisher Scientific
Loading Buffer, TRIS-Glycine Native Sample Buffer (4X)
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HEPES lysis buffer with NP-40 (2X)
Supplier: Thermo Fisher Scientific
HEPES lysis buffer with NP-40 (2X), Thermo Fisher Scientific
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BES, 0.5 M buffer solutions
Supplier: Thermo Fisher Scientific
BES buffer solution, 0,5 M, pH value: 6,0, Colourless, Liquid
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Cesium chloride - lanthanum chloride buffer, for flame AAS, TraceSELECT™, Fluka™
Supplier: Honeywell Chemicals
TraceSELECT™, for flame atomic absorption spectrometry.
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MNE buffer, pH 6,5
Supplier: Thermo Fisher Scientific
MNE buffer, pH 6.5, Thermo Fisher Scientific
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Phosphate, 0,2 M buffer solution, pH 6,8
Supplier: Thermo Fisher Scientific
Phosphate buffer soln, 0,2 M, pH value: 6,8, Liquid
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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer) pH 8,5
Supplier: PanReac AppliChem
Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer) pH 8,5
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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/l), Millipore®
Supplier: Merck Millipore (Calbiochem)
Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)
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EDTA solution, 0,5 M, molecular biology grade, Calbiochem®, Millipore®
Supplier: Merck Millipore (Calbiochem)
Sterile-filtered solution of 0,5 M EDTA in H₂O treated with diethyl pyrocarbonate (DEPC). Used in molecular biology applications.
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