Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Buffer, TBE solution 5X concentrate (TRIS-borate-EDTA buffer)
Supplier: Thermo Fisher Scientific
Running Buffer, TBE buffer solution 5X concentrate (TRIS-borate-EDTA buffer)
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Protein extraction reagent, BugBuster® HT, Millipore®
Supplier: Merck Millipore (Novagen)
BugBuster® HT Protein Extraction Reagent combines BugBuster® Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster® HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from E. coli without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.
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CHES, 0.5 M buffer solutions
Supplier: Thermo Fisher Scientific
2-(Cyclohexylamino)ethanesulfonic acid, CHES.
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TAE buffer, 10X solution, molecular biology grade, ultrapure
Supplier: Thermo Fisher Scientific
10X TAE solution consisting of 400 mM Tris and 0,01 M EDTA in high purity dH₂O adjusted to pH 8,3. Solution is 0,2 μm filtered and dispensed into durable, square Nalgene® bottles.
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Nitrocellulose stripping buffer (10X)
Supplier: Thermo Fisher Scientific
Electrophoresis, Nitrocellulose Stripping Buffer, 10X
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TE buffer, 1X, Molecular Biology Grade, Ultrapure
Supplier: Thermo Fisher Scientific
Use directly to re-suspend and/or dilute purified DNA or RNA.
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G-Biosciences ACD (Acid Citrate Dextrose) Solutions for Anticoagulants
Supplier: G-Biosciences
ACD (Acid Citrate Dextrose) Solution A and ACD (Acid Citrate Dextrose) Solution B are anticoagulants for whole blood and also act as acidifying agents to platelet poor plasma.
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G-Biosciences Acetate Buffer
Supplier: G-Biosciences
Acetate buffer at pH 5,0 and 5,5 are supplied as ready to use solutions that eliminate the calculation, formulation and weighing errors.
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TRIS-buffered saline (TBS, 10X) pH 7,4, for Western blot
Supplier: Thermo Fisher Scientific
Washing buffer, TRIS-buffered saline (TBS, 10X)
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20X SSPE, liquid, Corning®
Supplier: Corning
20X Saline-Sodium-Phosphate-EDTA (SSPE) buffer has applications in nucleic acid hybridization and other molecular biology work.
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TEA, 0,2 M buffer soln., pH 8,0
Supplier: Thermo Fisher Scientific
TEA buffer solution, 0,2 M, pH value: 8,0, Colourless, Odourless, Liquid
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TRIS-glycine-SDS running buffer (10X), pH 8,3 test
Supplier: Thermo Fisher Scientific
Running Buffer, TRIS-glycine-SDS running buffer (10X)
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TRIS-buffered saline (TBS, 10X) pH 7,6
Supplier: Thermo Fisher Scientific
Washing buffer, TRIS-buffered saline (TBS, 10X)
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HEPES-buffered saline, pH 7,0 (2X for transfection)
Supplier: Thermo Fisher Scientific
Cell culture buffer, HEPES-buffered Saline
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TRIS-glycine native sample buffer (4X), pH 8,6
Supplier: Thermo Fisher Scientific
Loading Buffer, TRIS-Glycine Native Sample Buffer (4X)
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#: 83640.320
Supplier: VWR Chemicals
#: 31770.294
Supplier: VWR Chemicals
#: 1.00030.2500
Supplier: Merck
#: 83645.320
Supplier: VWR Chemicals
#: 20060.320
Supplier: VWR Chemicals