Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

EDTA, 0.5M pH 8.0, Corning®

Supplier: Corning

EDTA (ethylenediaminetetraacetic acid) is a polyamino carboxylic acid. It is a colorless, water-soluble solid and is useful as a chelating agent due to its ability to "sequester" metal ions such as Ca2+ and Fe3+. These cations are responsible for various undesirable effects in molecular biological and cell culture processes. After being bound by EDTA, these metal ions remain in the solution but exhibit diminished reactivity. The use of EDTA therefore allows for greater control over many experiments.

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10X Tris buffered saline, liquid, Corning®

Supplier: Corning

Tris Buffered Saline (TBS) is an isotonic, non-toxic buffer used in some biomedical techniques to maintain pH within a relatively narrow range.

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Buffers, glycylglycine, 0,2 M solutions

Supplier: Thermo Fisher Scientific

Glycylglycine, buffer solution, 0,2 M, pH value: 8,0, Colourless, Odourless, Liquid

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Cell lysis buffers

Supplier: Rockland Immunochemicals

Cell lysis buffer is used to lyse cells under non-denaturing conditions.

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Phosphate, 0,5M buffer solution, pH 7,5

Supplier: Thermo Fisher Scientific

Phosphate buffer soln, 0,5 M, pH value: 7,5, Liquid

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POPSO, 0.2 M buffer solution, pH 8.0

Supplier: Thermo Fisher Scientific

POPSO buffer solution, 0,2 M, pH value: 8,0, Liquid

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Transformation buffer-2, pH 7,5

Supplier: Thermo Fisher Scientific

Cloning Buffer, Transformation buffer

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TRIS-buffered saline (TBS, 20X) pH 7,4

Supplier: Thermo Fisher Scientific

Washing buffer, TRIS-buffered saline (TBS, 20X)

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HEPES, 1,0 M buffer solutions

Supplier: Thermo Fisher Scientific

HEPES buffer solution, 1 mol/L, pH value: 9,0, Liquid

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TRIS-buffered saline (TBS, 10X) pH 7,4

Supplier: Thermo Fisher Scientific

Washing buffer, TRIS-buffered saline (TBS, 10X)

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MOPS-SDS running buffer (20X)

Supplier: Thermo Fisher Scientific

Running Buffer, MOPS-SDS running buffer (20X)

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Buffer, potassium phosphate, 0,2 M solution

Supplier: Thermo Fisher Scientific

Potassium phosphate, 0,2M buffer solution, pH 7,0, Liquid

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TRIS-buffered saline (TBS, 10X), with 1% Triton® X-100

Supplier: Thermo Fisher Scientific

Washing buffer, TRIS-buffered saline (TBS, 10X), with 1% Triton® X-100

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pH buffer solutions, referenced at 20 °C, Fluka™

Supplier: Honeywell Chemicals

pH Buffer solution, Glycine, pH value: 13,00, ±0.05, Reference temperature: 20 °C, Fluka™, Plastic bottle, 6×1 L

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Buffer, TAE solution 10X concentrate (TRIS-acetate-EDTA buffer)

Supplier: Thermo Fisher Scientific

Running Buffer, TAE buffer solution 10X concentrate (TRIS-acetate-EDTA buffer)

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TE buffer, pH 8,1, RNAse free

Supplier: Thermo Fisher Scientific

Trizma is used in the formulation of buffer solutions in the pH range between 7,5 and 8,5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification.