Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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MNE buffer, pH 6,5

Supplier: Thermo Fisher Scientific

MNE buffer, pH 6.5, Thermo Fisher Scientific

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TRIS-buffered saline (TBS, 10X) pH 7,4, for Western blot

Supplier: Thermo Fisher Scientific

Washing buffer, TRIS-buffered saline (TBS, 10X)

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TRIS-glycine-SDS running buffer (10X), pH 8,5

Supplier: Thermo Fisher Scientific

Running Buffer, TRIS-glycine-SDS running buffer (10X)

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HEPES-buffered saline, pH 7,0 (2X for transfection)

Supplier: Thermo Fisher Scientific

Cell culture buffer, HEPES-buffered Saline

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Phosphate, 0,5M buffer solution, pH 6,5

Supplier: Thermo Fisher Scientific

Phosphate buffer soln, 0,5 M, pH value: 6,5, Liquid

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Protein extraction reagent, BugBuster® HT, Millipore®

Supplier: Merck Millipore (Novagen)

BugBuster® HT Protein Extraction Reagent combines BugBuster® Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster® HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from E. coli without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.

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Buffer, TBE solution 5X concentrate (TRIS-borate-EDTA buffer)

Supplier: Thermo Fisher Scientific

Running Buffer, TBE buffer solution 5X concentrate (TRIS-borate-EDTA buffer)

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Phosphate, 0,2 M buffer solution, pH 7,6

Supplier: Thermo Fisher Scientific

Phosphate buffer soln, 0,2 M, pH value: 7,6, Liquid

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CHES, 0.5 M buffer solutions

Supplier: Thermo Fisher Scientific

2-(Cyclohexylamino)ethanesulfonic acid, CHES.

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TRIS-glycine native sample buffer (4X), pH 8,6

Supplier: Thermo Fisher Scientific

Loading Buffer, TRIS-Glycine Native Sample Buffer (4X)

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Stacking buffer (4X)

Supplier: Thermo Fisher Scientific

Electrophoresis, Stacking Buffer, 4X

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NP-40 lysis buffer, low salt

Supplier: Thermo Fisher Scientific

NP-40 lysis buffer, low salt, Thermo Fisher Scientific

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Phosphate-buffered saline (PBS, 10X), pH 7,4, for Western blot

Supplier: Thermo Fisher Scientific

Washing buffer, Phosphate-buffered saline (PBS, 10X)

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G-Biosciences Acetate Buffer

Supplier: G-Biosciences

Acetate buffer at pH 5,0 and 5,5 are supplied as ready to use solutions that eliminate the calculation, formulation and weighing errors.

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His·Bind® buffer kit, Novagen®

Supplier: Merck Millipore (Novagen)

The His·Bind buffer kit is a set of pre-tested buffers designed for use with IDA-based His·Bind resin for convenient, rapid one-step purification of proteins by metal chelation chromatography.