Supplier: Thermo Fisher Scientific

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

Supplier: Honeywell Chemicals

pH Buffer solution, Borate, pH value: 10,00, ±0.05, Reference temperature: 20 °C, Fluka™, Plastic bottle, 6×1 L

Supplier: Merck

TISAB, in general, is mixed with samples, as it helps in maintaining the ionic strength and pH constant. It is mainly used to decomplex metal-F complexes.

Supplier: HyClone products (Cytiva)

HyClone AdvanceSTEM ES Qualified HEPES provides maintenance of physiological pH in cell culture despite changes in carbon dioxide.

Supplier: Merck

TISAB solution for fluoride determination

Supplier: G-Biosciences

A 10X concentrated Tris Buffered Saline supplemented with Tween 20.

Supplier: Merck Millipore (Calbiochem‎)

Sterile-filtered solution of 0,5 M EDTA in H₂O treated with diethyl pyrocarbonate (DEPC). Used in molecular biology applications.

Supplier: EKSIGENT

SCIEX Low pH SDS Sample Buffer, 140 mL (100 mM Tris-HCl, pH 6.8, 1% SDS) allows you to use SCIEX’s Gold Standard CE-SDS with a low pH SDS sample buffer. Use of this buffer can increase non-reduced purity by decreasing method induced fragmentation. Less variability and lower RSDs means less troubleshooting and faster method development.

Supplier: Thermo Fisher Scientific

Washing buffer, PBS, 10X solution, molecular biology grade, ultrapure

Supplier: NOVILYTIC

Proteometer-CV reconstitution reagent.

Supplier: NOVILYTIC

Fluorescent reagent portion of the Proteometer-CV full kit. This is sold individually due to shipping and storage conditions.

Supplier: Cytiva

Efficient cell lysis and protein extraction are key steps for achieving high-quality results in downstream applications. The 2-D protein extraction buffer trial kit is designed for those who experiment for establishing a suitable extraction and solubilization buffer for their protein samples.

Supplier: Cytiva

A major limitation of membrane hybridisation is speed. At commonly used probe concentrations (2 to 10 ng/ml), hybridisation normally takes 16 hours to detect single-copy gene sequences in mammalian DNA. Use of Rapid-hyb buffer reduces this time to 2 hours using similar probe concentrations.

Supplier: Cytiva

Ab buffer kit contains prepared buffer concentrates for binding, washing, and elution of IgG according to recommended protocols.

Supplier: G-Biosciences

Water based salt solution containing sodium phosphate monobasic and dibasic at a fixed pH.

Supplier: MP Biomedicals

Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions:

Supplier: Rockland Immunochemicals

Contains immunoanalytical grade non-fat dry milk. Blocks non specific binding of proteins in various immunological assays. Ideal for use in western blotting and ELISA methods.

Supplier: Merck

TISAB, in general, is mixed with samples, as it helps in maintaining the ionic strength and pH constant. It is mainly used to decomplex metal-F complexes.

Supplier: Merck

TISAB III solution for fluoride determinations

Supplier: Cytiva

Protein A/G HP SpinTrap™ Buffer kit is optimised for standard affinity-based enrichment/immunoprecipitation according to the protocol provided with protein A HP SpinTrap™, protein G HP SpinTrap™, and Ab SpinTrap™ columns.

Supplier: OMEGA BIO-TEK

Component of the Mag-Bind® plant DNA 96 plus kit.

Supplier: G-Biosciences

A protein solubilisation buffer for 2D analysis must solubilise proteins effectively, without disturbing the native charge of the proteins. Urea based solubilisation buffers solubilise proteins effectively, however can modify the native charge of the proteins, due to carbamylation. Urea exists in equilibrium with ammonium cyanate that modifies α- and ε-amino groups, inducing changes in the isoelectric point of proteins leading to artifactual results.

Supplier: Thermo Fisher Scientific

TBS is used in Western blotting protocols.

Supplier: Thermo Fisher Scientific

Thermo Fisher Scientific

Supplier: MP Biomedicals

HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a general purpose zwitterionic organic chemical buffering agent which does not bind magnesium, calcium, manganese(II) or copper (II) ions.

Supplier: G-Biosciences

Tissue PE LB™ has been developed for extraction of total biologically active, soluble proteins from animal tissues. Tissue PE LB™ is based on an organic buffer and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Tissue PE LB™. Tissue PE LB™ has been tested for use with a wide variety of fresh and frozen animal tissues.

Supplier: G-Biosciences

Mammalian Cell PE LB™ has been developed for extraction of total biologically active, soluble proteins from mammalian cultured cells. The Mammalian Cell PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents, phosphatase and protease inhibitors may be added into Mammalian Cell PE LB™. Mammalian Cell PE LB™ has been tested on a wide variety of mammalian cells and can be used for both suspension and adherent cells.

Supplier: Thermo Fisher Scientific

This extraction reagent is formulated to effectively lyse yeast and bacterial cells to extract and solubilise proteins for analysis.

Supplier: MP Biomedicals

EDTA disodium salt, (2%) solution, in phosphate buffered saline without calcium and magnesium. Used as a gentle non-enzymatic cell dissociation reagent.

Supplier: Thermo Fisher Scientific

Contains 0,9 M tris base, 0,9 M phosphate and 200 mM EDTA.