Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)
Supplier: Thermo Fisher Scientific
Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)
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Buffer, TRIS solution pH 7,5 (1 mol/L)
Supplier: Cayman Chemical
Cloning Buffer, TRIS buffer solution pH 7,5 (1 mol/l)
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Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)
Supplier: Thermo Fisher Scientific
Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)
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Lithium nitrate, ionisation buffer solution, Specpure®
Supplier: Thermo Fisher Scientific
Lithium Nitrate Buffer Solution
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Electrophoresis running buffers
Supplier: G-Biosciences
There are many electrophoresis running buffers including: TAE running buffer (50×), TBE running buffer (10×), TBE-urea sample buffer, MOPS running buffer (10×), and MOPS/ SDS running buffer (20×). These include the SDS-PAGE sample loading buffer, SDS-PAGE running buffer, native sample loading buffer, tris glycine native gel running buffer, tricine sample buffer, tris-tricine, tris-tricine-SDS, MES SDS running buffer, MOPS/ SDS running buffer, IEF anode buffer, IEF cathode buffer, zymogram sample buffer, zymogram renature buffer, zymogram development buffer and the discontinuous buffer system.
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Dry buffer packs, Carbonate-Bicarbonate Buffer, JAW™
Supplier: G-Biosciences
Premixed buffer powders that simply require adding deionised water to generate a ready-to-use buffer.
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Blocking buffers, FirstChoice™
Supplier: G-Biosciences
FirstChoice™ Blocking Buffer is ideal as a first choice for optimization of new assays and systems, or when determining the optimal blocking buffer for elimination of non specific binding sites in ELISA, blotting, immunohistochemistry and other applications. Buffers are conveniently supplied in widely used TBS (Tris-buffered saline at pH 7,5) and PBS (phosphate-buffered saline at pH 7,5) buffers as well as in separate formulations containing Tween® 20 for improving blocking efficiencies.
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G-Biosciences TBE Running Buffer (10X)
Supplier: G-Biosciences
The primary use of TBE running buffer is in agarose electrophoresis for the separation of nucleic acids (DNA and RNA).
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Gram Negative lysis buffer
Supplier: G-Biosciences
Gram-negative lysis buffer is an extraction buffer for soluble proteins from Gram-negative bacteria. It is a proprietary improvement on the lysozyme based lysis in combination with various salts and agents, which allows extraction of soluble proteins. This buffer has been tested for use with several widely used bacteria including E.coli strains.
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G-Biosciences TBE-Urea Sample Buffer
Supplier: G-Biosciences
Loading buffer to resolve single-stranded DNA oligos or RNA into sharp, distinct bands.
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Antigen Retrieval Buffer (100X Tris Buffer, pH 10.0), Abcam
Supplier: Abcam
Antigen Retrieval Buffer (100X Tris Buffer, pH 10.0) is a special solution that enables rehydrate and target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step.
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Background suppressor system (Permeabilising), TrueBlack® IF
Supplier: Biotium
A buffer set that blocks non-specific antibody binding as well as direct interaction of fluorescent dyes on antibodies with cells or tissue sections.
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Nuclear Extraction Kit, Abcam
Supplier: Abcam
Nuclear extraction kit provides a simple and selective method for nuclear protein extraction and fractionation from cells or tissue samples in just 1 hour. This kit includes all necessary reagents to obtain high yields of nuclear proteins suitable for various downstream applications. - Cited in over 255 publications - High retention of enzyme activity – contains 80% less salt than conventional kits and no SDS, allowing better enzyme activity retention - Suitable for western blotting, protein-DNA binding assays, nuclear enzyme assays, and more - Fast, simple procedure that ensures optimized nuclear protein isolation
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AEC Substrate Buffer, Abcam
Supplier: Abcam
This product is manufactured by BioVision, an Abcam company and was previously called 2123 AEC Substrate Buffer.
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Buffer, PBS tablets
Supplier: PanReac AppliChem
PBS Tablets for preparation of 200 ml phosphate buffered saline (PBS). Composition of 140 mM sodium chloride, 2,7 mM potassium chloride and 10 mM phosphate.
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Buffer, TBS solution 10X concentrate (TRIS buffered saline) pH 7,4
Supplier: Cayman Chemical
Formulation: Concentrated TBS (250 mM Tris-base; pH 7,4; 1,37 M sodium chloride and 27 mM KCl).



