Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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Enzyme storage buffer in PBS and glycerol, autoclaved

Supplier: Thermo Fisher Scientific

Enzyme Storage Buffer in PBS and Glycerol

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ADA, 0,2 M Buffer Solutions

Supplier: Thermo Fisher Scientific

N-(2-Acetimido)iminodiacetic acid monosodium salt (ADA) buffer solutions PH values 6,0 to 7,5.

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LDS-sample buffer (4X), non-reducing

Supplier: Thermo Fisher Scientific

LDS-sample buffer (4X), non-reducing is used in SDS-polyacrylamide gel electrophoresis (SDS-PAGE). It is used in denaturing gels and is compatible with both coomassie and silver staining and Western blotting applications.

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Phosphate-buffered saline (PBS, 10X), no potassium

Supplier: Thermo Fisher Scientific

Phosphate-buffered saline (PBS), No potassium, 10X, Liquid

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IEF Sample buffer (4X) pH 3 to 7 with lysine

Supplier: Thermo Fisher Scientific

Loading Buffer, IEF Sample Buffer (4X) with Lysine

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Phosphate-buffered saline (PBS, 10X), pH 7,4

Supplier: Thermo Fisher Scientific

Phosphate-buffered saline (PBS), 10X, pH value: 7,4, Colourless, Liquid

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Lysis buffer, 0,5% Tween 20, pH 8,0, for isolation of His-tag modified proteins

Supplier: Thermo Fisher Scientific

Lysis buffer, 0.5% Tween 20, pH 8.0, for isolation of His-tag modified proteins, Thermo Fisher Scientific

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Buffers, concentrated solutions, Fluka™

Supplier: Honeywell Chemicals

pH Buffer, concentrate, pH value: 5,00, ±0.02, Ampoule

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Phosphate buffer solution pH 7,00 (20 °C), Fluka™

Supplier: Honeywell Chemicals

Potassium dihydrogen phosphate / di-sodium hydrogen phosphate

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Laemmli SDS sample buffer, reducing (4X)

Supplier: Thermo Fisher Scientific

Loading Buffer, Laemmli SDS Sample Buffer, reducing, 4X

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10% SDS (Sodium Dodecyl Sulfate), Corning®

Supplier: Corning

Sodium Dodecyl Sulfate (SDS) is an anionic detergent with applications in SDS-PAGE protein analysis and nucleic acid purification. It is a wetting agent that is effective in both acid and alkaline solutions, and is most often used as a protein and lipid solubilization reagent. SDS is also a powerful protein denaturant. This 10% solution is prepared in 18 megaohm water and is 0.2µm filtered.

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G-Biosciences Tris-EDTA-NaCl Buffer

Supplier: G-Biosciences

Tris-EDTA-NaCl buffer solution at pH 8,0.

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GST buffer kit

Supplier: Cytiva

GST Buffer Kit contains carefully prepared buffer concentrates for binding, washing, and elution of GST-tagged proteins according to recommended protocols.

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10X Phosphate Buffered Saline (PBS), Sterile, Dulbecco's Formula, without Magnesium and Calcium

Supplier: MP Biomedicals

Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions: 1. Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance; 2. Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism; 3. Combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism; 4. Provides a buffering system to maintain the medium within the physiological pH range (7.2 - 7.6)

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Tris-Glycine (TG) buffer,, MP Biomedicals

Supplier: MP Biomedicals

Tris-glycine buffer is used for western blotting and gel electrophoresis.

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Buffer Capsules

Supplier: Greisinger

Cost-effective alternative: Buffer capsules and working sets. With buffer capsules, you can produce a 100 ml buffer solution in the most cost-effective manner. A particular advantage of the capsules is that their storage life is several years longer than that of ready-to-use solutions.