Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Phosphate-buffered saline (PBS, 10X), no potassium
Supplier: Thermo Fisher Scientific
Phosphate-buffered saline (PBS), No potassium, 10X, Liquid
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Tris-HCl, 1M solution, pH 8,0, Molecular Biology Grade, Ultrapure
Supplier: Thermo Fisher Scientific
1M solution of Tris, Ultrapure in high purity distilled H₂O, adjusted to pH 8,0.
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Buffer, SSC solution 20X concentrate (saline-sodium citrate)
Supplier: LONZA
Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)
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Buffer, TRIS, 1,0 M Solution, pH 9,0
Supplier: Thermo Fisher Scientific
TRIS buffer solution pH 9 (1 mol/L), Odourless, Liquid
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10% SDS (Sodium Dodecyl Sulfate), Corning®
Supplier: Corning
Sodium Dodecyl Sulfate (SDS) is an anionic detergent with applications in SDS-PAGE protein analysis and nucleic acid purification. It is a wetting agent that is effective in both acid and alkaline solutions, and is most often used as a protein and lipid solubilization reagent. SDS is also a powerful protein denaturant. This 10% solution is prepared in 18 megaohm water and is 0.2µm filtered.
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Maleate buffers, 0,2 M solutions, pH 5,0 - 7,0
Supplier: Thermo Fisher Scientific
Maleate buffer, 0,2 M, pH value: 5,5, Colourless, Liquid
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Phosphate-buffered saline (PBS, 10X), pH 7,4
Supplier: Thermo Fisher Scientific
Phosphate-buffered saline (PBS), 10X, pH value: 7,4, Colourless, Liquid
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Separating or resolving buffer (4X)
Supplier: Thermo Fisher Scientific
Separating Buffer, Separating or Resolving Buffer, 4X
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Lysis buffer, 0,5% Tween 20, pH 8,0, for isolation of His-tag modified proteins
Supplier: Thermo Fisher Scientific
Lysis buffer, 0.5% Tween 20, pH 8.0, for isolation of His-tag modified proteins, Thermo Fisher Scientific
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IEF Sample buffer (4X) pH 3 to 7 with lysine
Supplier: Thermo Fisher Scientific
Loading Buffer, IEF Sample Buffer (4X) with Lysine
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Phosphate buffer solution pH 7,00 (20 °C), Fluka™
Supplier: Honeywell Chemicals
Potassium dihydrogen phosphate / di-sodium hydrogen phosphate
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G-Biosciences Tris-EDTA-NaCl Buffer
Supplier: G-Biosciences
Tris-EDTA-NaCl buffer solution at pH 8,0.
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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer) pH 8,5
Supplier: PanReac AppliChem
Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer) pH 8,5
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Buffer, PBS (1X, Dulbecco's) powder pack
Supplier: PanReac AppliChem
Powder mixture to prepare PBS buffer (1X) according to Dulbecco.
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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/l), Millipore®
Supplier: Merck Millipore (Calbiochem)
Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)
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EDTA solution, 0,5 M, molecular biology grade, Calbiochem®, Millipore®
Supplier: Merck Millipore (Calbiochem)
Sterile-filtered solution of 0,5 M EDTA in H₂O treated with diethyl pyrocarbonate (DEPC). Used in molecular biology applications.
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#: 83640.320
Supplier: VWR Chemicals
#: 31770.294
Supplier: VWR Chemicals
#: 1.00030.2500
Supplier: Merck
#: 83645.320
Supplier: VWR Chemicals
#: 20060.320
Supplier: VWR Chemicals