You searched for: Assays

Supplier: Thermo Fisher Scientific

Pierce™ Phosphoprotein Phosphate Estimation Assay Kit enables classification and identification of proteins as phosphorylated (serine and threonine), as well as semi-quantitative assessment of the phosphorylation level.

Supplier: Thermo Fisher Scientific

Micro BCA™ protein assay kit is a specialised version of Pierce™ BCA protein assay for determining the protein concentration of dilute samples.

Supplier: OZ BIOSCIENCES

OZBlue cell viability kit is a ready to use assay system based on the fluorimetric/colorimetric quantification of metabolic activity in living cells. Resazurin dye is used as an indicator of cell viability.

Supplier: Thermo Fisher Scientific

Easy-Titer® IgG assay kits are simple, mix-and-read assays that allow quick and accurate determination of concentrations from 15 to 300 ng/ml of IgG.

Supplier: AAT BIOQUEST

The Cell Meter™ cell adhesion assay kit is a fast and sensitive assay for measuring cell-cell or cell-surface adhesion for a variety of cell types.

Supplier: Cayman Chemical

A sensitive tool for measuring free fatty acids in plasma, serum, and urine.

Supplier: Thermo Fisher Scientific

The bradford protein assay is the preferred colorimetric assay for quantifying total protein concentration. The Pierce™ coomassie plus protein assay is a ready-to-use, reducing agent-compatible, improved bradford assay reagent to quickly measure (A595 nm) total protein concentration compared to a protein standard.

Supplier: Thermo Fisher Scientific

Pierce™ 660 nm Protein Assay is a ready to use, detergent- and reducing agent-compatible assay reagent to quickly measure (A660 nm) total protein concentration compared to a protein standard.

Supplier: OZ BIOSCIENCES

ROS Detection Assay Kit is a cell-based assay designed for measuring Reactive Oxygen Species (ROS) activity within a cell using the cell-permeable fluorogenic probe 2', 7'-Dichlorodihydrofluorescein diacetate (DCF-DA). Once DCF-DA has diffused into cells it is deacetylated by cellular esterases to a non-fluorescent compound and rapidly oxidised by ROS into DCF. DCF is highly fluorescent and can be detected by microscopy, titration in microplate and also cytometry (ex 485/em 535). The fluorescence intensity that results is proportional to the ROS levels within the cell.

Supplier: Thermo Fisher Scientific

The Thermo Scientific™ Compat-Able™ Coomassie Plus Protein Assay Kit eliminates interfering substances from protein assay samples to make them compatible with BCA and Bradford assays.

Supplier: SIGMA ALDRICH MICROSCOPY

Bicinchoninic acid is used for the determination of protein concentration. It is responsible for the making of intense purple complex with cuprous ion (Cu+) in the presence of alkaline conditions. The amount of Cu+ is in relation with the protein concentration.

Supplier: Biotium

Apoptosis and necrosis are two processes by which cells die. Apoptosis is an active, regulated disassembly of the cell from within. During apoptosis, phosphatidylserine is translocated from the inner to the outer surface of the cell, allowing the dying cell to be engulfed by phagocytic cells. Annexin V is a 35 kD Ca2+-dependent phospholipid binding protein with a high affinity for phosphatidylserine.

Supplier: OZ BIOSCIENCES

The luciferase assay kit is the most sensitive analytical tools for measuring gene expression.

Supplier: Thermo Fisher Scientific

The Modified Lowry Protein Assay Kit is the preferred colorimetric assay for quantifying total protein concentration.

Supplier: VWR Chemicals

The Protein Precipitation Kit utilises a simple trichloroacetic acid (TCA) based method to precipitate proteins in complex mixtures. Only protein in the sample is pelleted, while interfering salts and surfactants remain in the supernatant. A gentle acetone wash removes TCA and co-precipitants allowing the end-user to recover the protein in a small volume of buffer.

Supplier: AAT BIOQUEST

Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR).

Supplier: Biotium

AccuGreen™ kit is specific for dsDNA and can quantify DNA samples in the range of 0,1 to 10 ng/μl.

Supplier: OZ BIOSCIENCES

BCA Protein Assay kit (BCA-PAK) is useful for colorimetric detection and quantification of total protein content, even in the presence of detergents.

Supplier: Thermo Fisher Scientific

The Compat-Able™ Reagents remove salts, detergents, reducing agents and other substances from protein samples to eliminate interference with protein assays. The protocol selectively precipitates all of the protein, thereby allowing the non-protein components to be easily decanted and removed. The purified, precipitated protein is redissolved in water, making it ready for accurate determination of the protein concentration by standard protein assay methods. The procedure is accomplished in less than 10 min with just a few simple steps. The Compat-Able™ Preparation Set is available conveniently bundled with the Pierce BCA™ Protein Assay Kit.

Supplier: G-Biosciences

The Phosphatase Assay Kit is designed to measure the activity of phosphatases in biological samples and to screen for agonists and inhibitors of phosphatases. The Phosphatase Assay Kit uses para-nitrophenyl phosphate (pNPP), a chromogenic substrate for most phosphatases, including alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases.

Supplier: Thermo Fisher Scientific

Pierce BCA™ (bicinchoninic acid) reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA™ Protein Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA™ Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity.

Supplier: AAT BIOQUEST

Our Cell Meter™ assay kits are a set of tools for monitoring cell viability.

Supplier: G-Biosciences

CytoScan™ WST-1 Cell Proliferation Assay principle is based upon the reduction of the tetrazolium salt WST-1 to formazan by cellular dehydrogenases. The generation of the dark yellow coloured formazan is measured at 420 to 480 nm (optimal at 440 nm) and is directly correlated to cell number.

Supplier: Biotium

Stain microtubules in live cells with green fluorescence.

Supplier: AAT BIOQUEST

Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR).

Supplier: AAT BIOQUEST

Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR).

Supplier: AAT BIOQUEST

Cell Meter™ flow cytometric calcium assay kit provides fluorescence-based assays for detecting intracellular calcium mobilisation using a flow cytometer.

Supplier: OZ BIOSCIENCES

Bradford Protein Assay Kit (B-Pak) is a straightforward and rapid kit for determining the concentration of proteins in solution. This ready to use kit is provided with 1X reagent and 2 sets of BSA standards which means no dilution, filtration or calculation required. B-Pak is provided with adapted protocols for all experimental conditions – micro and macroassays.

Supplier: AAT BIOQUEST

Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR).

Supplier: Abcam

Pyruvate dehydrogenase (PDH) Protein Quantity Dipstick Assay Kit is an immunoassay kit for the detection of Pyruvate dehydrogenase (PDH) Protein Quantity in Mouse, Rat, Cow, Human in Cell culture extracts, Tissue samples.