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Assays

An assay is an investigative procedure in laboratory medicine, pharmacology, environmental biology, and molecular biology for qualitatively assessing or quantitatively measuring the presence or functional activity of the analyte, or the entity being targeted by the assay. Generally, assays involve biological materials or phenomena which tend to be intrinsically more complex in composition or in behavior than what can be handled by traditional chemical analysis and titration.

PMA™, PMAxx™, Real-Time PCR Viability Kit

PMA™, PMAxx™, Real-Time PCR Viability Kit

Supplier: Biotium

Viability PCR With PMA and PMAxx™ dyes

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Amplite® Fluorimetric Tyrosinase Assay Kit

Amplite® Fluorimetric Tyrosinase Assay Kit

Supplier: AAT BIOQUEST

Tyrosinase is an enzyme expressed across a vast range of species from bacteria and fungi to animals.

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Prostaglandin Screening Library I

Prostaglandin Screening Library I

Supplier: Cayman Chemical

For screening of PGF analogs.

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Bucculite™ XdU Cell Proliferation Fluorescence Imaging Kit

Bucculite™ XdU Cell Proliferation Fluorescence Imaging Kit

Supplier: AAT BIOQUEST

Provides an alternative to anti-BrdU antibody-based assay and EdU click chemistry-based assay

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Foxp3/transcription factor staining buffer

Foxp3/transcription factor staining buffer

Supplier: Tonbo Biosciences

Foxp3/transcription factor staining buffer contains specially formulated buffers and solutions for optimal resolution and low background in your analysis of nuclear antigens by flow cytometry. This complete kit contains three components for use in these staining protocols for detection of nuclear antigens such as Foxp3 and ROR gamma. Foxp3/transcription factor fix/perm concentrate (4x) 50 ml. A concentrated solution which, when diluted with Foxp3/transcription factor fix/perm diluent (1x) provides best results in protocols for intranuclear staining of transcription factors using fluorescently conjugated antibodies.

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Cell Meter™ Cellular Senescence Activity Assay Kit *Red Fluorescence*

Cell Meter™ Cellular Senescence Activity Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Cellular Senescence is an irreversible growth arrest triggered in order to prevent growth in DNA damaged cells.

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Cardiotoxicity kit, EarlyTox™

Cardiotoxicity kit, EarlyTox™

Supplier: Molecular Devices

The EarlyTox™ Cardiotoxicity Kit provides a fast, simple and reliable fluorescence based method for identifying cardiotoxic compounds in a biorelevant assay using stem-cell derived cardiomyocytes.

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Amplite® Colorimetric Glucose-6-Phosphate Assay Kit

Amplite® Colorimetric Glucose-6-Phosphate Assay Kit

Supplier: AAT BIOQUEST

Glucose-6-phosphate (G6P) is a key intermediate for glucose transport into cells.

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Amplite® Colorimetric Butyrylcholinesterase Activity Assay Kit

Amplite® Colorimetric Butyrylcholinesterase Activity Assay Kit

Supplier: AAT BIOQUEST

Butyrylcholinesterase (EC 3.1.1.8; BChE), also known as pseudocholinesterase or plasma cholinesterase, is mainly synthesised in liver and present in blood.

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Yeast β-galactosidase assay kit, Pierce™

Yeast β-galactosidase assay kit, Pierce™

Supplier: Thermo Fisher Scientific

Yeast β-Galactosidase Assay Kit is specially formulated for yeast cells and ideal for identifying protein interactions in vivo using two-hybrid systems.

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Amplite® Colorimetric Xanthine Oxidase Assay Kit

Amplite® Colorimetric Xanthine Oxidase Assay Kit

Supplier: AAT BIOQUEST

Xanthine oxidase (XO)is an enzyme that catalyzes the oxidation of hypoxanthine to xanthine and can further catalyse the oxidation of xanthine to uric acid.

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Amplite® Colorimetric Glucose Oxidase Assay Kit

Amplite® Colorimetric Glucose Oxidase Assay Kit

Supplier: AAT BIOQUEST

The glucose oxidase is a dimeric protein that catalyses the oxidation of beta-D-glucose into hydrogen peroxide and D-glucono-1,5-lactone, which is hydrolysed to gluconic acid.

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Live or Dead™ Cell Viability Assay Kits *Green/Red Dual Fluorescence*

Live or Dead™ Cell Viability Assay Kits *Green/Red Dual Fluorescence*

Supplier: AAT BIOQUEST

This Live or Dead™cell viability uses two fluorogenic indicators: calcein AM for viable cells and a cell-impermeable DNA-binding dye for the cells with compromised membranes.

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Amplite® Colorimetric Xanthine Assay Kit

Amplite® Colorimetric Xanthine Assay Kit

Supplier: AAT BIOQUEST

Xanthine is a purine base found in most human body tissues and fluids.

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Amplite® Colorimetric Beta-Galactosidase Assay Kit

Amplite® Colorimetric Beta-Galactosidase Assay Kit

Supplier: AAT BIOQUEST

E. coli beta-galactosidase is a 464 kD tetramer.

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Amplite® Luminometric Peroxidase (HRP) Assay Kit

Amplite® Luminometric Peroxidase (HRP) Assay Kit

Supplier: AAT BIOQUEST

Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio.

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Amplite® Fluorimetric Glycerol Assay Kit

Amplite® Fluorimetric Glycerol Assay Kit

Supplier: AAT BIOQUEST

Glycerol is a precursor for the synthesis of triglycerides and phospholipids in liver and adipose tissue.

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Amplite® Colorimetric NADPH Assay Kit

Amplite® Colorimetric NADPH Assay Kit

Supplier: AAT BIOQUEST

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

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Amplite® Universal Fluorimetric Kinase Assay Kit *Red Fluorescence*

Amplite® Universal Fluorimetric Kinase Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

The Amplite® Universal Kinase Assay Kit is based on the monitoring of ADP formation, which is directly proportional to enzyme phoshphotransferase activity and is measured fluorimetricaly. This kit provides a fast, simple, and homogeneous assay for measure kinases activities. The characteristics of its high sensitivity (<0.2 uM ADP), broad ATP tolerance (1-300 uM), non-antibody based, non-radioactive and no-wash method to detect the amount of ADP produced as a result of enzyme activity make it an ideal kit for determining kinase Michaelis-Menten kinetics and for screening and identifying kinase inhibitors. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation with no separation steps required.

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Amplite® Fluorimetric Proteasome 20S Activity Assay Kit *Green Fluorescence*

Amplite® Fluorimetric Proteasome 20S Activity Assay Kit *Green Fluorescence*

Supplier: AAT BIOQUEST

Our Amplite® Fluorometric 20S Proteasome Assay Kit is a homogeneous fluorescent assay that measures the chymotrypsin-like protease activity associated with the proteasome complex either in cultured cells or cell lysates.

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ExoBrite™ WGA EV Staining Kits

Supplier: Biotium

ExoBrite™ WGA EV staining kits were designed to overcome some of the challenges of EV detection, particularly in flow cytometry. ExoBrite™ WGA EV stains bind to molecules in the EV membrane for bright, specific staining, with little background.

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Epigenetics Screening Library

Epigenetics Screening Library

Supplier: Cayman Chemical

For screening a variety of epigenetic compounds.

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Amplite® Colorimetric L-Lactate Assay Kit

Amplite® Colorimetric L-Lactate Assay Kit

Supplier: AAT BIOQUEST

Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid.

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Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Blue Fluorescence*

Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Blue Fluorescence*

Supplier: AAT BIOQUEST

Hydrogen peroxide is a reactive oxygen metabolic by-product that serves as a key regulator for a number of oxidative stress-related states.

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Cell Meter™ Fluorimetric Intracellular Peroxynitrite Assay Kit *Optimized for Flow Cytometry*

Cell Meter™ Fluorimetric Intracellular Peroxynitrite Assay Kit *Optimized for Flow Cytometry*

Supplier: AAT BIOQUEST

Peroxynitrite (ONOO-) is a strong oxidizing species and a highly active nitrating agent.

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Gaussia-firefly luciferase dual assay kit, Pierce™, Thermo Scientific

Gaussia-firefly luciferase dual assay kit, Pierce™, Thermo Scientific

Supplier: Thermo Fisher Scientific

The Thermo Scientific Pierce Gaussia-Firefly Luciferase dual assay kit provides the necessary reagents to simultaneously detect intracellular Gaussia and red firefly luciferase activity in mammalian whole cell lysates.

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IMAP kinase, phosphatase, and phosphodiesterase assays, molecular devices

Supplier: Molecular Devices

IMAP® technology provides a homogeneous assay applicable to a wide variety of kinases without regard for substrate peptide sequence.

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Cell Meter™ Fluorimetric Mitochondrial Superoxide Activity Assay Kit *Green Fluorescence*

Cell Meter™ Fluorimetric Mitochondrial Superoxide Activity Assay Kit *Green Fluorescence*

Supplier: AAT BIOQUEST

Mitochondria are major producers of cellular superoxide.

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Ascorbate Assay Kit

Ascorbate Assay Kit

Supplier: Cayman Chemical

A kit for measuring ascorbate.

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Amplite® Fluorimetric Ascorbic Acid Assay Kit

Amplite® Fluorimetric Ascorbic Acid Assay Kit

Supplier: AAT BIOQUEST

L-Ascorbic Acid (also called Vitamin C) is a critical metabolite for both plant and animals in cell division, growth and defense.

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