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Assays

An assay is an investigative procedure in laboratory medicine, pharmacology, environmental biology, and molecular biology for qualitatively assessing or quantitatively measuring the presence or functional activity of the analyte, or the entity being targeted by the assay. Generally, assays involve biological materials or phenomena which tend to be intrinsically more complex in composition or in behavior than what can be handled by traditional chemical analysis and titration.

Pierce™ Micro BCA™ Protein Assay Kit and Reagents

Pierce™ Micro BCA™ Protein Assay Kit and Reagents

Supplier: Thermo Fisher Scientific

Micro BCA™ protein assay kit is a specialised version of Pierce™ BCA protein assay for determining the protein concentration of dilute samples.

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Cell Meter™ Cell Adhesion Assay Kit

Cell Meter™ Cell Adhesion Assay Kit

Supplier: AAT Bioquest

The Cell Meter™ cell adhesion assay kit is a fast and sensitive assay for measuring cell-cell or cell-surface adhesion for a variety of cell types.

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Glutaminase (GLS1) Inhibitor Screening Kit (Fluorometric)

Glutaminase (GLS1) Inhibitor Screening Kit (Fluorometric)

Supplier: Abcam

Sensitive, reproducible assay for initial screening of potential glutaminase inhibitors.

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Cell viability assay kits, OZBlue

Cell viability assay kits, OZBlue

Supplier: OZ BIOSCIENCES

OZBlue cell viability kit is a ready to use assay system based on the fluorimetric/colorimetric quantification of metabolic activity in living cells. Resazurin dye is used as an indicator of cell viability.

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ROS Detection assay kit

ROS Detection assay kit

Supplier: OZ BIOSCIENCES

ROS Detection Assay Kit is a cell-based assay designed for measuring Reactive Oxygen Species (ROS) activity within a cell using the cell-permeable fluorogenic probe 2', 7'-Dichlorodihydrofluorescein diacetate (DCF-DA). Once DCF-DA has diffused into cells it is deacetylated by cellular esterases to a non-fluorescent compound and rapidly oxidised by ROS into DCF. DCF is highly fluorescent and can be detected by microscopy, titration in microplate and also cytometry (ex 485/em 535). The fluorescence intensity that results is proportional to the ROS levels within the cell.

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Coomassie Plus™ protein assay kit, Compat-Able™

Supplier: Thermo Fisher Scientific

The Thermo Scientific™ Compat-Able™ Coomassie Plus Protein Assay Kit eliminates interfering substances from protein assay samples to make them compatible with BCA and Bradford assays.

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Phosphatase assay kit

Phosphatase assay kit

Supplier: G-Biosciences

The Phosphatase Assay Kit is designed to measure the activity of phosphatases in biological samples and to screen for agonists and inhibitors of phosphatases. The Phosphatase Assay Kit uses para-nitrophenyl phosphate (pNPP), a chromogenic substrate for most phosphatases, including alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases.

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Luciferase assay kit

Luciferase assay kit

Supplier: OZ BIOSCIENCES

The luciferase assay kit is the most sensitive analytical tools for measuring gene expression.

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Modified lowry protein assay kit, Pierce™

Modified lowry protein assay kit, Pierce™

Supplier: Thermo Fisher Scientific

The Modified Lowry Protein Assay Kit is the preferred colorimetric assay for quantifying total protein concentration.

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Pierce™ Coomassie Plus (Bradford) Protein Assay Reagent

Pierce™ Coomassie Plus (Bradford) Protein Assay Reagent

Supplier: Thermo Fisher Scientific

The bradford protein assay is the preferred colorimetric assay for quantifying total protein concentration. The Pierce™ coomassie plus protein assay is a ready-to-use, reducing agent-compatible, improved bradford assay reagent to quickly measure (A595 nm) total protein concentration compared to a protein standard.

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Free Fatty Acid Fluorometric Assay Kit

Free Fatty Acid Fluorometric Assay Kit

Supplier: Cayman Chemical

A sensitive tool for measuring free fatty acids in plasma, serum, and urine.

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Screen Quest™ Luminometric Calcium Assay Kit

Screen Quest™ Luminometric Calcium Assay Kit

Supplier: AAT Bioquest

Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR).

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DuraClone Dry Unitized Antibody Panels For Flow Cytometry

DuraClone Dry Unitized Antibody Panels For Flow Cytometry

Supplier: Beckman Coulter

The dry DURAClone antibody panels are designed for clinical research studies. Every assay tube or well contains a layer with the dried-down antibody panel unitized for a single test.

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VWR® Protein Precipitation Kit

VWR® Protein Precipitation Kit

Supplier: VWR Chemicals

The Protein Precipitation Kit utilises a simple trichloroacetic acid (TCA) based method to precipitate proteins in complex mixtures. Only protein in the sample is pelleted, while interfering salts and surfactants remain in the supernatant. A gentle acetone wash removes TCA and co-precipitants allowing the end-user to recover the protein in a small volume of buffer.

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Pierce™ 660 nm Protein Assay Kit and Reagent

Pierce™ 660 nm Protein Assay Kit and Reagent

Supplier: Thermo Fisher Scientific

Pierce™ 660 nm Protein Assay is a ready to use, detergent- and reducing agent-compatible assay reagent to quickly measure (A660 nm) total protein concentration compared to a protein standard.

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MESG *Phosphate Assay Reagent*

MESG *Phosphate Assay Reagent*

Supplier: AAT Bioquest

In the presence of inorganic phosphate MESG is converted to 2-amino-6-mercapto-7-methlpurine by purine nucleoside phosphorylase (EC 2.4.2.1) with absorption wavelength shift to red.

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Apoptosis and necrosis quantitation kits

Apoptosis and necrosis quantitation kits

Supplier: Biotium

Apoptosis and necrosis are two processes by which cells die. Apoptosis is an active, regulated disassembly of the cell from within. During apoptosis, phosphatidylserine is translocated from the inner to the outer surface of the cell, allowing the dying cell to be engulfed by phagocytic cells. Annexin V is a 35 kD Ca2+-dependent phospholipid binding protein with a high affinity for phosphatidylserine.

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dsDNA quantitation kits for the Qubit® fluorometer, AccuGreen™, high-sensitivity and broad-range

dsDNA quantitation kits for the Qubit® fluorometer, AccuGreen™, high-sensitivity and broad-range

Supplier: Biotium

AccuGreen™ kit is specific for dsDNA and can quantify DNA samples in the range of 0,1 to 10 ng/μl.

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Pierce™ BCA™ Protein assay kits and reagents

Pierce™ BCA™ Protein assay kits and reagents

Supplier: Thermo Fisher Scientific

Pierce BCA™ (bicinchoninic acid) reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA™ Protein Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA™ Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity.

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Cell Meter™ Fluorimetric Cell Cytotoxicity Assay Kit

Cell Meter™ Fluorimetric Cell Cytotoxicity Assay Kit

Supplier: AAT Bioquest

Our Cell Meter™ assay kits are a set of tools for monitoring cell viability.

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BCA protein assay kit (BCA-PAK)

BCA protein assay kit (BCA-PAK)

Supplier: OZ BIOSCIENCES

BCA Protein Assay kit (BCA-PAK) is useful for colorimetric detection and quantification of total protein content, even in the presence of detergents.

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Bradford protein assay kits (B-Pak)

Bradford protein assay kits (B-Pak)

Supplier: OZ BIOSCIENCES

Bradford Protein Assay Kit (B-Pak) is a straightforward and rapid kit for determining the concentration of proteins in solution. This ready to use kit is provided with 1X reagent and 2 sets of BSA standards which means no dilution, filtration or calculation required. B-Pak is provided with adapted protocols for all experimental conditions – micro and macroassays.

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Bicinchoninic acid disodium salt hydrate

Supplier: SIGMA ALDRICH MICROSCOPY

Bicinchoninic acid is used for the determination of protein concentration. It is responsible for the making of intense purple complex with cuprous ion (Cu+) in the presence of alkaline conditions. The amount of Cu+ is in relation with the protein concentration.

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Cell Meter™ Flow Cytometric Calcium Assay Kit

Cell Meter™ Flow Cytometric Calcium Assay Kit

Supplier: AAT Bioquest

Cell Meter™ flow cytometric calcium assay kit provides fluorescence-based assays for detecting intracellular calcium mobilisation using a flow cytometer.

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Screen Quest™ Rhod-4 No Wash Calcium Assay Kit

Screen Quest™ Rhod-4 No Wash Calcium Assay Kit

Supplier: AAT Bioquest

Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR).

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Screen Quest™ Fluo-8 No Wash Calcium Assay Kit

Screen Quest™ Fluo-8 No Wash Calcium Assay Kit

Supplier: AAT Bioquest

Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR).

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Screen Quest™ Fura-2 No Wash Calcium Assay Kits

Screen Quest™ Fura-2 No Wash Calcium Assay Kits

Supplier: AAT Bioquest

Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR).

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Quantitative peroxide assay kit (aqueous), Pierce™

Quantitative peroxide assay kit (aqueous), Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Quantitative Peroxide Assay Kit (Aqueous) detects and measures hydrogen peroxide levels in biological samples using an iron and xylenol orange* (XO) reagent.

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Cytoplasmic membrane labelling kits, CellBrite™

Cytoplasmic membrane labelling kits, CellBrite™

Supplier: Biotium

CellBrite kits are designed to label cell cytoplasmic membranes with a fluorescent membrane dye pre-dissolved as an optimised staining solution. By combining CellBrite membrane stains, it is possible to label multiple cell populations with distinctive colours, a capability useful for the studies of cell-cell interactions.

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Quantitative peroxide assay kit (lipid), Pierce™

Quantitative peroxide assay kit (lipid), Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Quantitative Peroxide Assay Kit (Lipid) detects and measures hydrogen peroxide levels in biological samples using an iron and xylenol orange* (XO) reagent.

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