Nucleic Acid Modification and Labelling

Supplier: Abcam

Abcam's Oligonucleotide Conjugation Kit (ab218260) offers several standout features: - Rapid Conjugation: enables generation of antibody-oligonucleotide conjugates in less than 2 hours. - High Efficiency: achieves high levels of antibody and oligonucleotide recovery, with conjugation efficiency ~ 95-100%. - Versatility: Suitable for conjugating antibodies to single-stranded oligonucleotides (10-120 bases) and double-stranded oligonucleotides (up to 80 bases), providing flexibility for various research needs

Supplier: Thermo Fisher Scientific

T4 DNA Polymerase catalyses the 5'→3' synthesis of DNA from a primed single-stranded DNA template.

Supplier: AGILENT

These QuikChange II kits provide improved fidelity, while maintaining greater than 80% mutation efficiency for single site mutagenesis.

Supplier: G-Biosciences

T4 UvsX DNA recombinase mediates DNA strand exchange between homologous chromosomes. The protein forms a right-handed nucleoprotein complex on single ssDNA called the presynaptic filament that can search for homology in duplex DNA and pair the recombining DNA molecules to form a DNA joint molecule. This process is aided by ssDNA binding proteins and recombination mediators. The filament is then elongated by DNA polymerase with the newly synthesised strand displacing the old strand. The newly synthesised duplex DNA can then act as a template for the next cycle to achieve exponential amplification of dsDNA. E.coli cells expressing recombinant T4 UvsX gene.

Supplier: AGILENT

The fastest and latest generation of the market-leading QuikChange kits speeds up the protocol for performing single and multiple site-directed mutagenesis to less than 3 hours.

Supplier: FINNZYMES REAGENTS

Phusion® Site-Directed Mutagenesis Kit is a versatile and efficient tool for introducing point mutations, insertions, or deletions in any type of plasmid DNA.

Supplier: AAT Bioquest

ReadiView™ Blue Real-Time PCR Visualisation Dye is a 200X concentrated, ready-to-use, inert dye that enhances the visibility of real-time PCR reactions for more accurate pipetting and plate loading.

Supplier: AAT Bioquest

Tyramide signal amplification (TSA) is a technique used to enhance the signal of target molecules, such as antibodies or nucleic acid probes.

Supplier: AAT Bioquest

Tyramide signal amplification (TSA) is a technique used to enhance the signal of target molecules, such as antibodies or nucleic acid probes, in immunohistochemistry and in situ hybridisation.

Supplier: MP Biomedicals

Hybrisol VII

Supplier: Genscript

ABE8e is an innovative adenine base editor designed to enable precise genetic modifications by converting A-T base pairs to G-C base pairs without introducing double-stranded breaks or requiring donor DNA templates.​ABE8e contains eight additional mutations in the TadA domain, augments the effectiveness and applicability of adenine base editing.