You searched for: Cell Culture Supplements and Additives

Supplier: Corning

Synthemax® II-SC Substrate is an easy to use, self-coating material for creating a unique synthetic surface that mimics the natural cell environment. This surface supports the adhesion and expansion of stem cells in their undifferentiated state and directs differentiation into specialised cell types. In addition, it offers a synthetic, xeno-free alternative to biological coatings and/or feeder cell layers used in hES and iPS cell culture, whenever needed and appropriate.

Supplier: Corning

In our Starter Kit we have combined 30 ml of Corning 3D Clear with the four buffer solutions required to process 3D tissue cultures for immunolabelling. All you need to do is add your immunolabels and nuclear stains. This kit comes with enough material to process three 96-well plates of 3D cell culture models.

Supplier: Merck Millipore (Calbiochem‎)

Recombinant, human epidermal growth factor is a protein expressed in E. coli which is identical to human EGF except for an additional N-terminal methionine.

Supplier: Corning

Growth Factor Reduced (GFR) formulation is well suited for applications that require a more highly defined basement membrane preparation.

Supplier: Thermo Fisher Scientific

A 1X stock solution contains 500 μM AEBSF.HCl, 150 nM aprotinin, 1 μM E-64, 0,5 mM EDTA and 1 μM leupeptin hemisulfate. Reconstitute each vial with 1 ml water to obtain a 100X stock solution.

Supplier: Corning

Corning® Cell Recovery Solution allows for the recovery of cells cultured on Corning® Matrigel® Basement Membrane Matrix for subsequent biochemical analysis. Corning® Cell Recovery Solution depolymerises Corning® Matrigel® Matrix gels without enzymatic digests and lengthy incubation periods at high temperatures. Cells are released without damage thereby avoiding biochemical changes during incubation and digestion of extracellular portions of cell-surface receptors and adhesion molecules.

Supplier: Corning

Cellstripper® is a non-enzymatic cell dissociation solution formulated with a proprietary mixture of chelators that gently dislodge adherent cells in tissue culture. It is an alternative to trypsin when used with either serum-free or serum-supplemented media. Because Cellstripper® is a gentler reagent than trypsin, cells can be exposed to Cellstripper for longer periods of time. Cellstripper® achieves superior results in comparison to enzymatic dissociation products.

Supplier: Corning

Corning® Nu-Serum™ growth media supplement provides a low-protein alternative to newborn calf, foetal bovine, and other sera routinely used for cell culture. The low-protein content facilitates protein purification, virus production, monoclonal antibody production and screening, and increases the frequency of successful transfection of cells.

Supplier: Corning

Corning® Cell-Tak™ Cell and Tissue Adhesive is a formulation of polyphenolic proteins extracted from Mytilus edulis (marine mussel). These proteins are the key components of the glue secreted by the mussel to anchor itself to solid substrates in the marine environment. Corning® Cell-Tak™ is used to attach cells or tissue sections to many types of surfaces, including plastic, glass, metal, PTFE, and biological materials. It can simplify the manipulation of biological samples for a number of in vitro techniques, including in situ hybridisation, immunoassays, microinjection, immunohistochemistry, and establishing primary cells in culture. Corning® Cell-Tak™ is biocompatible and demonstrates no species specificity.

Supplier: MP Biomedicals

Accutase solution is a trypsin replacement that contains both proteolytic and collagenolytic enzymes for cell detachment.

Supplier: MP Biomedicals

Basal Medium Eagle (BME) Vitamin Concentrate (100X), without L-glutamine

Supplier: Cytiva

The double-stranded polyribonucleotide poly(I)·poly(C) induces interferon in rabbit cells, and there has been interest in its effectiveness for inducing human interferon in vitro.

Supplier: MP Biomedicals

Trace Element Solution 2 is a 1000X sterile filtered solution, suitable for use with serum-free media; ideal for plant cell culture.

Supplier: US Biological

Enrichment supplement recommended for the cultivation of Mycobacteria sp. OADC enrichment medium is used with Middlebrook 7H10 Mycobacteria Agar (Powder).

Supplier: MP Biomedicals

A broad spectrum protease inhibitor cocktail which covers both endopeptidases and exopeptidases, non-specific and specific. The cocktail consists of four major protease inhibitors such as AEBSF, Sodium EDTA, Leupeptin and Pepstain A.

Supplier: Cytiva

Sample preparation often requires the inhibition of protease activity. Protease inhibitor mix combines competitive and non competitive protease inhibitors, which protect proteins from proteolysis during purification from animal tissues, plant tissues, yeast and bacteria.

Supplier: MP Biomedicals

FastGro allows culturing cells in vitro without the use of serum and without any animal or human derived compound. FastGro is a fully defined additive, avoiding the need for serum, for a wide range of cell culture practices. Since FastGro is chemically defined it avoids lot-to-lot variations. All components in FastGro are highly purified and identified chemical compounds.

Supplier: FACELLITATE

The BIOFLOAT™ FLEX coating solution guarantees the creation of surfaces without unspecific protein or cell binding.

Supplier: MP Biomedicals

Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells.

Supplier: MP Biomedicals

Brewers yeast is often used as a source of B-complex vitamins, chromium, and selenium. It is also can be used to bake bread, ferment wine, beer and ethanol production.

Supplier: Tonbo Biosciences

Lymphocyte Separation Medium (LSM) is a sterile-filtered iso-osmotic solution designed for the in vitro isolation of lymphocytes from diluted whole blood.

Supplier: MP Biomedicals

Most cells can synthesise non-essential amino acids (NEAA) through glucoaminolysis, glycolysis, or the TCA cycle. When these amino acids concentration levels are low, cells need to consume more glucose and glutamine in the media and produce excess by-products, which might affect cell growth. Adding NEAA into the cell culture medium could mitigate the potential side effects caused by self-production of these non-essential amino acids.

Supplier: THERMO FISHER DIAGNOSTICS

Egg yolk tellurite emulsion