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79 results for Extraction Buffers | Avantor

Extraction Buffers

Extraction Buffers

Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.

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Protein solubilisation buffer

Protein solubilisation buffer

Supplier: G-Biosciences

FOCUS™ protein solubilisation buffer is a dry, urea-based buffer for protein solubilisation. It is a pre-mixed formulation of urea, thiourea, CHAPS and non detergent sulphobetaine for maximum solubilising strength.

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Protein extraction kit, yeast PE LB™

Protein extraction kit, yeast PE LB™

Supplier: G-Biosciences

Yeast PE LB™ kit has been developed for extraction of biologically active, soluble proteins from yeast cells. Yeast PE LB™ is a proprietary improvement on the lyticase (Zymolyase®) based spheroplast preparation and extraction of soluble proteins from yeast cells. Yeast PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins.

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Y-PER™ plus Yeast Protein Extraction Reagent, Dialysable

Y-PER™ plus Yeast Protein Extraction Reagent, Dialysable

Supplier: Thermo Fisher Scientific

This extraction reagent is formulated to effectively lyse yeast and bacterial cells to extract and solubilise proteins for analysis.

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DNA/RNA clean-up buffers

Supplier: OMEGA BIO-TEK

Binding buffer XP2, Omega Bio-Tek

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ReadiUse™ Mammalian Cell Lysis Buffer *5X*

ReadiUse™ Mammalian Cell Lysis Buffer *5X*

Supplier: AAT Bioquest

All our ReadiUse™ reagents require minimal hands-on time.

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Mammalian protein extract reagents, M-PER™

Mammalian protein extract reagents, M-PER™

Supplier: Thermo Fisher Scientific

The M-PER Mammalian Protein Extraction Reagent is designed to provide highly efficient total protein extraction from cultured mammalian cells.

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Genomic DNA extraction buffers

Supplier: OMEGA BIO-TEK

SP2 buffer, Omega Bio-Tek

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Extraction buffers, FOCUS™

Extraction buffers, FOCUS™

Supplier: G-Biosciences

FOCUS™ Extraction Buffers are experimentally optimised concentrations of critical agents, buffering and stabilising agents, including urea, thiourea, Nonidet® P-40, CHAPS, and sulphobetaines (SB). They are designed to produce optimal protein extraction and improved spot resolution for 2D gel analysis.

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Nuclear and cytoplasmic extraction reagents, NE-PER

Nuclear and cytoplasmic extraction reagents, NE-PER

Supplier: Thermo Fisher Scientific

The NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours. The nuclear protein extraction method involves simple, stepwise lysis of cells and centrifugal isolation of nuclear and cytoplasmic protein fractions. A benchtop microcentrifuge, tubes and pipettors are the only tools required. The NE-PER Reagents efficiently solubilise and separate cytoplasmic and nuclear proteins into fractions with minimal cross-contamination or interference from genomic DNA and mRNA. The isolated proteins can be used to perform immunoassays as well as protein interaction studies such as mobility shift assays (EMSA), co-immunoprecipitation (Co-IP) and pull-down assays.

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ReadiUse™ Yeast Cell Lysis Buffer, 5X

ReadiUse™ Yeast Cell Lysis Buffer, 5X

Supplier: AAT Bioquest

ReadiUse™ Yeast Cell Lysis Buffer is a biochemical reagent designed for efficient yeast cell lysis and protein solubilisation in laboratory applications.

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ReadiUse™ Bacterial Cell Lysis Buffer *5X* (10 ml)

ReadiUse™ Bacterial Cell Lysis Buffer *5X* (10 ml)

Supplier: AAT Bioquest

Cell lysis refers to the breaking down of cells, and it is often used to analyze specific cellular compositions such as proteins, lipids, nucleic acids, reporter molecules, cell signal molecules and other small biomolecules.

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Y-PER™ Yeast Protein Extraction Reagent

Y-PER™ Yeast Protein Extraction Reagent

Supplier: Thermo Fisher Scientific

This reagent uses a mild detergent lysis procedure to efficiently release functionally active solubilised proteins.

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Protein extraction kit, Insect PE LB™

Protein extraction kit, Insect PE LB™

Supplier: G-Biosciences

Insect PE LB™ has been developed for extraction of total biologically active, soluble proteins from adherent or suspension cultured insect cells, including Sf9 and Sf21. Insect PE LB™ utilises a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. The Insect PE LB™ is fully compatible with downstream processes, such as electrophoresis and chromatography. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Insect PE LB™.

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Research buffers and reagents

Research buffers and reagents

Supplier: G-Biosciences

These DNase- and RNase-free buffers and reagents are designed for high quality research.

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Subcellular protein fractionation kit for tissues

Subcellular protein fractionation kit for tissues

Supplier: Thermo Fisher Scientific

This kit is ideal for cell fractionation and enrichment of proteins from five different cellular compartments within two hours.

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NucleoSpin® Kits for Genomic DNA Extraction

NucleoSpin® Kits for Genomic DNA Extraction

Supplier: MACHEREY-NAGEL

Combining selective purification technologies and chemistries, these are a comprehensive range of specialised DNA isolation kits for a broad range of diversified starting materials. The resulting high quality DNA serves as excellent basis for following demanding downstream applications.

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