You searched for: Enzymes
Enzymes accelerate, or catalyze, chemical reactions, and they are known to catalyze more than 5,000 biochemical reaction types. Most enzymes are proteins, although a few are catalytic RNA molecules. Choose specific enzymes for cleaving bonds, removing genomic DNA from RNA preparations, for producing fragments of proteins, or for use in ion exchange chromatography. Enzymes are used in the chemical industry and other industrial applications when extremely specific catalysts are required.
Benzonase® Salt Tolerant Endonuclease
Supplier: MERCK PRODUCTION CHEMICALS
The best-in-class solution for nucleic acid removal at high salt concentration in Bioprocessing. The Benzonase® Salt Tolerant endonuclease Emprove® Expert is a new enzyme, different in amino acids sequence from other Benzonase® endonuclease references. It was designed with state-of-the-art protein engineering capabilities, to ensure the highest activity at high salt concentrations.
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Bovine Ribonuclease A (from Pancreas), MP Biomedicals
Supplier: MP Biomedicals
Dissolves readily at 2 mg/ml in analytical grade water to give a clear colorless solution.
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Collagenase, MP Biomedicals
Supplier: MP Biomedicals
Collagenase can be solubilised in calcium-free solutions such as Hank's Balanced Salts or Earles Balanced Salt Solution.
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L-Glutamic dehydrogenase, MP Biomedicals
Supplier: MP Biomedicals
For Continuous Spectrophotometric Rate determination method: 0,3 to 0,6 unit/ml of L-Glutamic dehydrogenase in cold Triethanolamine buffer.
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Flavobacterium meningosepticum Proline specific endopeptidase, MP Biomedicals
Supplier: MP Biomedicals
Proline specific endopeptidase specifically cleaves peptide bonds on the carboxy side of proline residues.
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Bacillus cereus beta-Lactamase, MP Biomedicals
Supplier: MP Biomedicals
Activity: Each vial contains 500 units β-Lactamase I and 50 units β-Lactamase II Unit Definition: One unit is defined as the amount of enzyme which will hydrolyse 1,0 micromole of benzyl penicillin and 1,0 micromole of cephalosporin C per minute at 25 °C in the presence of EDTA pH 7,0.
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Bovine deoxyribonuclease i (from Pancreas), MP Biomedicals
Supplier: MP Biomedicals
Dissolve at a concentration of 1 mg/ml. Dilute further to a concentration of 20 to 60 U/ml immediately before the assay.
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Helix pomatia beta-Glucuronidase, MP Biomedicals
Supplier: MP Biomedicals
Beta-glucuronidases are members of the glycosidase family of enzymes that catalyse breakdown of complex carbohydrates. Human β-glucuronidase is a type of glucuronidase that catalyses hydrolysis of β-D-glucuronic acid residues from the non-reducing end of mucopolysaccharides, such as heparan sulfate. In human gut β-glucuronidase converts conjugated bilirubin to the unconjugated form for reabsorption.
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Peroxidase, MP Biomedicals
Supplier: MP Biomedicals
HRP is a plant glycohemoprotein belongs to the ferroprotoporphyrin group of peroxidases. It is used in biochemistry applications such as western blots, ELISA and Immunohistochemistry. It is used to amplify a weak signal and increase detectability of a target molecule, such as a protein. HRP labeled immunoglobulins are used as probes for the demonstration of tissue antigens and in enzyme immunoassay (EIA) systems for determination of soluble and insoluble antigens.
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Catalase, MP Biomedicals
Supplier: MP Biomedicals
Dissolves readily at 5 mg/ml in 0,05 M sodium/potassium phosphate pH 7,0 giving a clear green solution.
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Malt diastase, MP Biomedicals
Supplier: MP Biomedicals
DIASTASE from malt, 1X N.F. stable, 60 mesh powder. Ready for CE IVD labeling of clinical applications.
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Protocatechuate-3,4-dioxygenase, MP Biomedicals
Supplier: MP Biomedicals
Inhibitors : Ag⁺, Hg²⁺, PCMB; Optimum temperature: 60 to 65 °C; Thermal stability: below 50 °C (pH 6,0; 1 h).
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Trichoderma viride cellulase Y-C, MP Biomedicals
Supplier: MP Biomedicals
Cellulase Y-C removes cell walls from plant tissues in shorter incubation period without loss of biological activity of the materials.
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Synthetic Yeast Inorganic Pyrophosphatase (from E. coli)
Supplier: Abnova
Synthetic Yeast Inorganic Pyrophosphatase (from E. coli)
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Recombinant Tobacco Etch Virus (from E. coli)
Supplier: Abnova
Recombinant Tobacco Etch Virus (from E. coli)
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Neuraminidase (from Bacteria), MP Biomedicals
Supplier: MP Biomedicals
Neuraminidase of non-pathogenic streptococci, is prepared from the culture filtrate by Ammonium Sulfate precipitation and Ion-exchange chromatography.
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Pyruvate kinase, MP Biomedicals
Supplier: MP Biomedicals
Dissolves readily at 5 mg/ml in 0,06 M Tris/HCI pH 7,4 to give a clear colorless solution.
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Human catalase (from Erythrocytes), MP Biomedicals
Supplier: MP Biomedicals
Catalase activates the decomposition of hydrogen peroxide, a reactive oxygen species, into water and oxygen.
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Cholesterol oxidase, MP Biomedicals
Supplier: MP Biomedicals
CHOD is soluble in cold 50 mM potassium phosphate buffer, pH 7,0. Prepare solutions immediately before use.
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Malate dehrdrogenase, MP Biomedicals
Supplier: MP Biomedicals
Soluble in distilled water or dilute buffer; dissolves readily at 5 mg/ml in 0,1 M potassium phosphate pH 7,4.
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Formaldehyde dehydrogenase, MP Biomedicals
Supplier: MP Biomedicals
One unit will oxidise 1,0 µmole of formaldehyde to formic acid per minute at pH 7,5 and 37 °C.
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Lactase (from Aspergillus oryzae), MP Biomedicals
Supplier: MP Biomedicals
One unit will hydrolyse 1,0 µmole of o-nitrophenyl-beta-D-galactoside to o-nitrophenol per minute at pH 4,5 and 30 °C.
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Alcohol dehydrogenase, MP Biomedicals
Supplier: MP Biomedicals
Dissolves readily at 5 mg/ml in 0,01 M Sodium Phosphate pH 7,5 to give a clear colourless solution.
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alpha-Chymotrypsin, MP Biomedicals
Supplier: MP Biomedicals
One unit will hydrolyse 1,0 µmole of N-benzoyl-L-tyrosine ethyl ester (BTEE) per minute at pH 7,8 and 25 °C in the presence of ionised calcium.
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Arthrobacter luteus zymolyase, MP Biomedicals
Supplier: MP Biomedicals
Zymolyase 100T is used to lyse living yeast cell walls to produce protoplast or spheroplast of various strains of yeast cells. It may also be used for glucan hydrolysis.
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Pineapple Bromelain, MP Biomedicals
Supplier: MP Biomedicals
Bromelain is a glycoprotein which is a highly active thiol proteinase (non-specific cysteine protease), which splits glycine esters but not lysine esters; it is also a protein-digesting and milk-clotting enzyme. Substrates susceptible to bromelain include all of the common protein materials such as gelatin, casein, gluten, collagen, elastin, globulins an muscle fiber protein. It is a cysteine endopeptidase with broad specificity for cleavage of proteins. It may be from a stem or piece of fruit.
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Pig alpha-Amylase (from Pancreas), MP Biomedicals
Supplier: MP Biomedicals
One unit catalyses the liberation of 1 mmole reducing group (calculated as maltose per minute at pH 6,9 at 20 °C).
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Lipase, MP Biomedicals
Supplier: MP Biomedicals
One USP unit of lipase activity is contained in the amount of pancreatin that liberates 1,0 µEq of acid per minute at a pH of 9,0 at 37 °C under the conditions of the assay for lipase activity.
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Clostridium histolyticum Collagenase type IV (from Clostridium histolyticum), MP Biomedicals
Supplier: MP Biomedicals
Activators: Ca²⁺ is required. Inhibitors: Metal chelating agents such as cysteine, EDTA or o-phenanthroline but not DFP. It is also inhibited by a2-macroglobulin - a large plasma glycoprotein. The human skin enzyme is inhibited by human serum but granulocyte collagenase is not. Human serum contains a2-macroglobulin and a1-antitrypsin that may inhibit certain collagenases.
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Crotalus adamanteus Phosphodiesterase I, MP Biomedicals
Supplier: MP Biomedicals
Lyophilised in vials containing approximately 5 mg dry wt. and at least 100 units/vial (20 to 40 units/mg dry wt.).