You searched for: Enzymes

Enzymes accelerate, or catalyze, chemical reactions, and they are known to catalyze more than 5,000 biochemical reaction types. Most enzymes are proteins, although a few are catalytic RNA molecules. Choose specific enzymes for cleaving bonds, removing genomic DNA from RNA preparations, for producing fragments of proteins, or for use in ion exchange chromatography. Enzymes are used in the chemical industry and other industrial applications when extremely specific catalysts are required.

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J.T.Baker® Endonuclease

Supplier: Avantor

J.T.Baker® Endonuclease Biotech Reagent meets the strictest cGMP standards and is designed for the degradation of both single stranded and double stranded DNA and RNA. J.T.Baker® Endonuclease Biotech Reagent is used to ensure host cell DNA impurities are removed; driving process efficiency by lowering viscosity and preventing aggregation. J.T.Baker® Endonuclease Biotech Reagent is an enzyme based upon the native endonuclease of Serratia marcescens, enabling rapid clearance of residual DNA and RNA during the production and purification of both recombinant proteins and viral vectors. Non-animal origin. Absence of proteolytic activity. The purity of materials in non-negotiable. J.T.Baker® Endonuclease Biotech Reagent acts to degrade and eliminate extraneous genetic material, ensuring the pristine quality of your final product.

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Protease

Supplier: Rockland Immunochemicals

Protease S.aureus V8 (endoproteinase-glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids. In the presence of ammonium, the enzyme specificity is limited to glutamic sites. It has a molecular weight of 27000 daltons and optimum pH's of 4,0 and 7,8 with hemoglobin as the substrate. Enzyme activity is determined by the casein digestion assay.

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VWR® Native Bovine Trypsin (from Pancreas)

Supplier: VWR Chemicals

From bovine pancreas.

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VWR®, Aspergillus Glucose oxidase

Supplier: VWR Chemicals

Beta-D-glucose oxidase with high purity grade and storage in frozen condition.

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VWR®, Bovine Ribonuclease A (from Pancreas)

Supplier: VWR Chemicals

Ribonuclease A is an endoribonuclease that efficiently hydrolyses RNA from tissue or bacterial cell cultures.

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DENARASE® highly active endonuclease from Serratia marcescens

Supplier: C LECTA

DENARASE® is a highly active endonuclease from Serratia marcescens. This enzyme is used in biopharmaceutical manufacturing to eliminate plasmid and host cell DNA/RNA. DENARASE® is manufactured under GMP conditions and is widely applied during the purification of viral vectors for gene/cell therapies and vaccines.

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VWR®, Pronase E

Supplier: VWR Chemicals

Off-white to light brown powder from Streptomyces griseus.

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Pancreatin

Supplier: PanReac AppliChem

Pancreatin

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Aspergillus oryzae Protease

Supplier: TCI

Aspergillus oryzae Protease

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Aspergillus niger Pectinase

Supplier: TCI

Aspergillus niger Pectinase

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Soy bean beta-Amylase

Supplier: TCI

Soy bean beta-Amylase

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Recombinant uridine phosphorylase Salmonella typhimurium

Supplier: Apollo Scientific

Recombinant uridine phosphorylase Salmonella typhimurium

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Proteinase K

Supplier: AGILENT

Proteinase K is a broad spectrum protease from Tritirachium album that is ideal for preparing chromosomal DNA.

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Proteinase K solution

Supplier: PanReac AppliChem

Proteinase K is used to destruct proteins in cell lysates (tissue, cultured cells) and to liberate nucleic acids, since it very effectively digests DNases and RNases.

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Peroxidase, Horseradish, RZ3 (POD, HRP, HRPO)

Supplier: US Biological

Horseradish Peroxidase (HRP) is a hemoprotein catalysing the oxidation by hydrogen peroxide of a number of substrates such as ascorbate, ferrocyanide, cytochrome C and the leuko form of many dyes.

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Deoxyribonuclease I

Supplier: Rockland Immunochemicals

Deoxyribonuclease I (usually called DNase I), is an endonuclease coded by the human gene DNASE1. DNase I is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides. It acts on single-stranded DNA, double-stranded DNA, and chromatin.

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Bovine MSG-Trypsin™

Supplier: G-Biosciences

Trypsin is a serine endopeptidase that specifically cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues and typically there is little or no cleavage at arginyl-proline and lysyl-proline bonds. The distribution of these residues in proteins allows trypsin digestion to produce peptides that are readily identified by mass spectrometry.

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Human Recombinant EN-TEV Protease (from E. coli)

Supplier: Abcam

Recombinant EN-TEV Protease protein is a Tobacco etch virus Fragment protein, in the 2038 to 2279 aa range, expressed in Escherichia coli, with >=95% purity and suitable for SDS-PAGE, Size Exclusion Chromatography.

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Nuclease S1

Supplier: Rockland Immunochemicals

Nuclease S1 is an endonuclease that is active against single-stranded DNA and RNA molecules. Although its primary substrate is single-stranded, it can also introduce single-stranded breaks in double-stranded DNA-RNA or DNA-RNA hybrids. Nuclease S1 is used in the laboratory as a reagent in nuclease protection assays. It is used in removing single stranded tails from DNA molecules to create blunt ended molecules and opening hairpin loops generated during synthesis of double stranded cDNA.