"protein purification system"

Supplier: MILLIPORE

The Amicon® Pro purification system - A centrifugal tool collapsing protein purifcation with concentration and buffer exchange.

Supplier: Avantor

The J.T.Baker^® BAKERBOND^® PROchievA™ protein A resin offers superior dynamic binding capacity with excellent alkaline stability linked to Avantor's proprietary ligand.

Supplier: Avantor

Silica-based media used for the purification of proteins based on differential salt induced hydrophobicity. Mixed mode provides unique selectivity with its hydrophobic interaction and ion exchange function.

Supplier: OMEGA BIOTEK

The Mag-Bind® Bacterial DNA kit allows rapid and reliable isolation of high quality genomic DNA (gDNA) from a wide variety of bacterial species. Up to 0,5 ml gram positive or gram negative bacterial culture can be processed each time. The key to the system are the Mag-Bind® particles that avidly, but reversibly, bind DNA or RNA under certain optimal conditions allowing proteins and other contaminants to be removed.

Supplier: OMEGA BIOTEK

RNA-Solv® Reagent is a one reagent system for the isolation of total RNA from cells and tissues. The reagent, a single-phase solution consisting of phenol and guanidine isothiocyanate, is a modification of the single-step RNA isolation method developed by Chomczynski and Sacchi. The sample is homogenised and lysed in RNA-Solv® Reagent, which maintains the integrity of the RNA while disrupting and denaturing endogenous RNases and other cellular components. Extraction of the lysate with chloroform further denatures proteins and separates the mixture into an organic and an aqueous phase. RNA remains exclusively in the aqueous phase, and is subsequently recovered by isopropanol.

Supplier: OMEGA BIOTEK

The E.Z.N.A.® Insect DNA kit is designed for efficient recovery of genomic DNA up to 60 kb in size from insects, arthropods, roundworms, flatworms, invertebrates, as well as some plant tissue samples. The method is suitable for samples frozen or preserved in alcohol or DNE solution, and good results can be obtained with formalin preserved material. The procedure relies on the well established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with the selective DNA binding of HiBind matrix. This allows salts, proteins and other contaminants to be removed to yield high quality genomic DNA suitable for downstream applications such as endonuclease digestion, thermal cycle amplification, and hybridisation techniques.

Supplier: OMEGA BIOTEK

The E.Z.N.A.® bacterial DNA kit allows the rapid and reliable isolation of high quality total cellular DNA from a wide variety of bacterial species. This kit uses optimised lysis condition and up to 1×10⁹ bacterial cells can be processed for each column. There are no organic extractions, thus reducing plastic waste and hands on time to allow multiple samples to be processed in parallel. Bacterial cells are grown to log-phase and harvested. The cell wall is removed by lysozyme digestion and bead beating, followed by protease digestion. Following lysis, binding conditions are adjusted and the sample is applied to a HiBind DNA spin-column. Two rapid wash steps remove trace salts and protein contaminants, and DNA is finally eluted in water or elution buffer. Purified DNA can be directly used in downstream applications without the need for further purification.

Supplier: PanReac AppliChem

TRItidy G™ is based on the single step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples (Chomczynski, P. (1993), BioTechniques 15, p.532 - 537). RNA is selectively retained in the aqueous phase during acidic GuSCN/phenol extraction; DNA and proteins are isolated from the organic phase by ethanol and isopropanol precipitation, respectively.

Supplier: MILLIPORE

The Amicon® Pro device streamlines the affinity purification process by providing a single adaptable centrifugation unit capable of performing all steps in the affinity purification process. The device combines affinity-based spin column purification with downstream sample concentration and buffer exchange, eliminating the need for multiple sample transfers, thereby minimising protein loss.

Supplier: OMEGA BIOTEK

MicroElute® Clean Up system, designed for rapid DNA clean up.

Supplier: MIRUS

The flashBAC™ Baculovirus Expression System is designed for high yield protein production in insect and mammalian cells.

Supplier: OMEGA BIOTEK

Isolate DNA from plant or fungal samples using spin columns.

Supplier: OMEGA BIOTEK

Isolate DNA from molluscs and insects using spin columns.

Supplier: OMEGA BIOTEK

Isolate DNA from plant or fungal sample with organic solvents using spin columns.

Supplier: VWR Collection

These vacuum filter units are very useful in large volume samples separation and purification.

Supplier: OMEGA BIOTEK

Isolate viral RNA from nasopharyngeal swab specimens (dry or in VTM) using magnetic beads.