buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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J.T.Baker® Cell Lysis Solution, Ultrapure Bioreagent

Supplier: Avantor

J.T. Baker® Cell Lysis Solution. Optimized multi-component, ready-to-use solution for extraction and recovery of AAV particles from HEK293 or SF9 cells. The product offers quantitative recovery through efficient cell lysis and protection of vector particles against shear stress. The solution is formulated with non-ionic detergents which are octyl phenol ethoxylate free.

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J.T.Baker® Cell Lysis Solution, Biotech Reagent

Supplier: Avantor

J.T.Baker® Cell Lysis Solution Biotech Reagent is a cGMP pre-formulated and ready to use detergent optimized for the extraction and recovery of AAV particles from producer cell lines (HEK293 parental and suspension lines, SF9). J.T.Baker® Cell Lysis Solution is a proprietary blend of nonionic surfactants, free of octyl phenol ethoxylate, which demonstrates comparable performance industry standard detergents while addressing environmental concerns associated with REACH and downstream clearance concerns. Designed for Gene Therapy workflows, this formulation is easy to use and handle as it is ready-to-use, has low viscosity, does not require cold temperature shipping or storage, and is available in sizes to support processes from development to large scale commercial batches. Detail description provided in technical literature.

Sustainable Options Available

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Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)

Supplier: PanReac AppliChem

Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)

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Buffer, PBS (10X, Dulbecco's) powder pack

Supplier: PanReac AppliChem

Powder mixture to prepare PBS buffer (10X) according to Dulbecco.

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Tris-Borate-EDTA (TBE) buffer, 10X

Supplier: PanReac AppliChem

10X concentrated solution of 0,89 M Tris, 0,89 M boric acid and 0,02 M EDTA.Na₂, pH 8,3 ±0,2.

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Buffer, triethylammonium acetate, 1 M solution, pH 7,0

Supplier: PanReac AppliChem

Used for the purification of oligonucleotides.

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Buffer, TRIS solution pH 7,5 (1 mol/L)

Supplier: PanReac AppliChem

Cloning Buffer, TRIS buffer solution pH 7,5 (1 mol/l)

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Buffer, TRIS solution pH 8 (1 mol/L)

Supplier: PanReac AppliChem

Cloning Buffer, TRIS buffer solution (1 mol/l)

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Cell culture salts, salt solutions and buffers

Supplier: BIOWEST

These irrigating buffers and salt solutions are sterile, physiological balanced solutions intended for use in the maintenance of mammalian cells, where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro. Applications include the intermediate steps for cultivation of cells, like washing, centrifugation, suspending and counting, as well as for many analytical methods or biochemical treatments. Principally these solutions should be used to keep the cells in suspension only for minutes or a few hours maximum. These buffers and solutions are not cell culture media.

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RNA isolation buffers

Supplier: OMEGA BIOTEK

TRK lysis buffer

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PBS tablets

Supplier: PanReac AppliChem

Tablets for preparation of 100 ml phosphate buffered saline (PBS).

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Buffer, PBS tablets

Supplier: PanReac AppliChem

PBS Tablets for preparation of 200 ml phosphate buffered saline (PBS). Composition of 140 mM sodium chloride, 2,7 mM potassium chloride and 10 mM phosphate.

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Plasmid DNA buffers

Supplier: OMEGA BIOTEK

Solution 1, resuspension buffer

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E.Z.N.A.® RNALock stabilizer reagent

Supplier: OMEGA BIOTEK

The E.Z.N.A.® RNALock Stabilizer Reagent is an innovative reagent which rapidly lyses cells and tissues to inactivate RNases at room temperature. Simply submerge the samples, such as cell cultures, animal tissues, or white blood cells into the RNALock Stabilizer Reagent. The intact cellular RNA will be preserved even at elevated temperature. Samples can be stored in this reagent in excess of 12 months at −20 °C.

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Buffer powder pillows

Supplier: HACH

The unit-dose powder pillows assure buffer freshness and eliminate the possibility of contamination. Each powder pillow prepares 50 ml of buffer solutions of respective pH.