110 Results for: "Quantabio"

Supplier: Quantabio

The 10 mM dNTP mix is a solution of high purity deoxyribonucleoside triphosphates that has been functionally qualified for real-time quantitative PCR (qPCR). It is suitable for use in conventional end-point PCR, real time qPCR, first-strand cDNA synthesis, as well as other applications that require dNTP as substrate. The use of qPCR certified dNTPs is a simple but important measure to ensure consistent assay performance.

Supplier: Quantabio

sparQ PureMag beads is a fast and reliable nucleic acid purification system for reaction cleanup and size selection in Next Generation Sequencing (NGS) workflows. Based on the reversible nucleic acid-binding properties of magnetic beads; this product can be used to quickly remove primers, primer-dimers, unincorporated nucleotides, salts, adapters and adapter-dimers from NGS library prep reactions to improve downstream sequencing performance. sparQ PureMag beads allows excellent recovery of fragments greater than 100 bp without centrifugation or filtration. Consistent and reliable size selection can be achieved by simply adjusting the beads to sample ratio. This product is designed for both manual and automated processing, allowing seamless integration into existing workflows.

Supplier: Quantabio

Synthesis of cDNA and PCR amplification are carried out in the same tube without opening between procedures. These kits are optimised to deliver maximum RT-PCR efficiency and specificity, enabling unbiased co-amplification of low copy transcripts in the presence of higher copy reference genes. As a result the qScript™ One-Step qRT-PCR Kits offer improved sensitivity over other commercially available qPCR reagents. The qScript™ One-Step Master Mix maximises the activities of both reverse transcriptase and PCR enzymes, while minimising primer-dimer formation. High specificity is assured with AccuStart™ Taq DNA Polymerase, which is bound by monoclonal antibodies for superior hot-start. Upon heating to 95 °C, the antibodies denature irreversibly releasing fully active and unmodified Taq DNA polymerase. qScript™ One-Step qRT-PCR Kits are available for all Real-Time PCR instrument platforms, including those requiring normalisation with ROX reference dye.

Supplier: Quantabio

Rapid extraction and purification of high-quality total DNA from cultured cells or tissue.

Supplier: Quantabio

The qScript™ XLT One-Step RT-PCR Kit is a convenient and highly sensitive system for amplification of RNA templates up to 2 kb. cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. This system has been optimised to deliver maximum endpoint RT-PCR efficiency, sensitivity, and specificity.

Supplier: Quantabio

AccuStart™ II PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb. It contains all components, except primers and template. AccuStart™ II PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. A key component is AccuStart™ II Taq DNA polymerase which contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

Supplier: Quantabio

PerfeCTa® Multiplex qPCR SuperMix, low ROX is a 2X concentrated, ready to use reaction cocktail that contains all components, except primers, probe(s), and template for real time qPCR on Applied Biosystems 7500 or Stratagene MX series of real time PCR systems.

Supplier: Quantabio

qScript™ cDNA synthesis kit contains qScript™ Reverse Transcriptase, a mixture of a MMLV reverse transcriptase derivative and ribonuclease inhibitor protein, optimised for sensitive and reliable cDNA synthesis over a broad dynamic range of input RNA. cDNA synthesis reagents are provided in a variety of SuperMix and kit formats to suit specific applications.

Supplier: Quantabio

The Extracta™ DNA prep for PCR - tissue consists of a two component reagent kit for rapid and efficient extraction of PCR-ready genomic DNA from mammalian tissues. The kit has been demonstrated to work with mouse tail biopsies, ear punches and a variety of human tissue samples including hair, buccal cells and saliva.

Supplier: Quantabio

Q qPCR cycler uses a patented magnetic induction technology to rapidly heat samples, coupled with fan-forced air for cooling to acquire data in only 25 minutes. Available as four channel models, the robust optical system acquires all channels simultaneously and allows for running the fastest multiplexed assays.

Supplier: Quantabio

ACCUMELT HRM SUPERMIX 2X HIGH RESOLUTION MELTING ANALYSIS SUPERMIX FOR USE ON ANY HRM-CAPABLE INSTRUMENT, 2 X 1.25ML, 250 X 20UL FINAL REACTION VOLUME 1 * 2,5 ml

Supplier: Quantabio

QSCRIPTONE-STEP QRT-PCR KIT 1 * 50 Assays

Supplier: Quantabio

PerfeCTa SYBR Green FastMix for iQ is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except prim ers and template for real-time quantitative PCR (qPCR) on Bio-Rad iCycler iQ systems. 1 * 250 Assays

Supplier: Quantabio

qScript One-Step RT and 4x concentrated master mix contains all components except gene-specific primers and template for fast one-step real-time qRT-PCR on instruments that do not require a passive reference dye. Contains 100 x 20uL reactions (1 x 5 1 * 100 Assays

Supplier: Quantabio

qScript One-Step RT and 4x concentrated master mix contains all components except gene-specific primers and template for fast one-step real-time qRT-PCR on Applied Biosystems 7000, 7300, 7700, or 7900 and StepOne# instruments. Contains 100 x 20uL r 1 * 100 Assays

Supplier: Quantabio

SYBR GR FASTMIX LOW ROX 5000R+VWR PLATES 1 * 1 SET

Supplier: Quantabio

The PerfeCta NGS Library Quantification Kit uses real-time PCR to specifically quantify library molecules that possess the appropria te adapter tag at each end.  These are the suitable template molecules for either emulsion PCR used for the Ion Torre 1 * 1 KIT

Supplier: Quantabio

PerfeCTa qPCR ToughMix UNG ROX is a 2X concentrated ready-to-use reaction cocktail for PCR amplification of DNA templates that overc omes many known inhibitors of PCR. Inclusion of uracil DNA glycosylase (UNG), and substitution of dTTP with dUTP, pre 1 * 250 Assays

Supplier: Quantabio

KIT QPCR FASTMIX ROX 250RXN 1 * 250 Assays

Supplier: Quantabio

PerfeCTa SYBR Green SuperMix, 250×20 µL or 100x50µL reactions 1 * 1 KIT

Supplier: Quantabio

PerfeCTa SYBR Green SuperMix, ROX™, 250×20 µL or 100x50µL reactions 1 * 1 KIT

Supplier: Quantabio

Ultraplex 1-step toughmix is a ready-to-use, 4x-concentrated master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridization probe detection chemistries such as taqman 5-hydrolysis probes. first-strand cDNA synt 1 * 1 KIT

Supplier: Quantabio

5PRIME HOTMASTER TAQDNA POLYMERASE5000U 1 * 1 KIT

Supplier: Quantabio

5PRIME HOTMASTERMIX - 1000R 1 * 1 KIT

Supplier: Quantabio

DNase I, (RNase-free) is an endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5- phosphorylated and 3-hydroxylated ends (1,2). DNase I acts on single- and double-stranded DNA, chromatin and RNA:DNA 1 * 1.000 Assays

Supplier: Quantabio

One-Step ToughMix (2x) 1 * 50 ml

Supplier: Quantabio

KIT ONE STEP QRT-PCR LOW ROX 50RXN 1 * 50 Assays

Supplier: Quantabio

SYBR GR FASTMIX LOW ROX 1250R+VWR PLATES 1 * 1 SET

Supplier: Quantabio

SYBR GREEN FASTMIX 5000R+VWR PLATES 1 * 1 SET

Supplier: Quantabio

SYBR GREEN FASTMIX ROX 5000R+VWR PLATES 1 * 1 SET