203 Results for: "OMEGA BIOTEK"

Supplier: OMEGA BIOTEK

The E.Z.N.A.® soil DNA kit is formulated to isolate high purity cellular DNA from soil samples typically containing humic acid and inhibitors of PCR. This kit has been successfully used to isolate DNA from tough-to-lyse bacteria, fungi, and algae that inhabit a range of samples including clay, sandy, peaty, chalky, and loamy soil samples. Isolated DNA can be used for most downstream applications including PCR, southern blotting, and SNP analysis.

Supplier: OMEGA BIOTEK

The E.Z.N.A.® miRNA isolation kit uses a rapid procedure to isolate small RNAs, such as micro RNA (miRNA), small interfering RNA (siRNA), and small nuclear RNA (snRNA), from wide range of tissues and cells. The fast and efficient silica membrane based method isolates total RNA ranging in size from kilo based down to decamers. The kit also provides reagents and a procedure to enrich the population of RNAs that are 200 bases and smaller, which enhances the sensitivity of small RNA detection by solution hybridisation, Northern analysis, and other methods.

Supplier: OMEGA BIOTEK

Selected protease digestion releases microgram amounts of RNA from FFPE samples. The purified nucleic acids, although highly fragmented, are suitable for a variety of downstream genomic and gene expression analyses. E.Z.N.A® spin column-based kits are ideal for low throughput applications while Mag-Bind® magnetic beads-based kits are designed specifically for high throughput users with automation capability.

Supplier: OMEGA BIOTEK

The E.Z.N.A.® Viral RNA Kit is designed for the isolation of viral RNA from cell free fluids such as plasma, serum, urine and cell culture supernatant. The procedure completely removes contaminants and enzyme inhibitors making viral RNA isolation fast, convenient and reliable. The kit is also suitable for the isolation of total RNA from cultured cells, tissues and bacteria. RNA purified using the E.Z.N.A.® Viral RNA method is ready for all downstream applications such as RT-PCR.

Supplier: OMEGA BIOTEK

Isolate DNA from soil and water samples using magnetic beads.

Supplier: OMEGA BIOTEK

The E.Z.N.A.® Fungal DNA kits allow for the rapid and reliable isolation of high quality total cellular DNA from a wide variety of fungal species without the need for organic extraction.

Supplier: OMEGA BIOTEK

This second generation plasmid miniprep kit yields 40 to 70 µg of high-quality DNA in less than 30 minutes using the same format as the E.Z.N.A.® Plasmid Mini Kit I. The increased DNA binding capacity of the HiBind® Mini Column II allows culture volumes of up to 15 ml to be used, thus bridging the gap between mini and midi prep protocols. Plasmid DNA can be readily and quickly isolated for most downstream applications such as routine screening, restriction enzyme digestion, and DNA sequencing.

Supplier: OMEGA BIOTEK

The E.Z.N.A.® HP Total RNA Isolation Kit provides a rapid and easy method for RNA isolation from a small amount of cultured eukaryotic cells or tissues. This kit allows single or simultaneous processing of multiple samples in less than 40 minutes. Normally, 1×10⁷ eukaryotic cells or 25 to 30 mg tissue can be used in a single experiment. There is no need for phenol/chloroform extractions, time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl. RNA purified using the E.Z.N.A.® HP Total RNA method is ready for applications such as RT-PCR, RT-qPCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

Supplier: OMEGA BIOTEK

The Mag-Bind® Endo-Free plasmid 96 kit combines the power of Mag-Bind® technology with the innovative ETR technology to deliver high quality endotoxin-free plasmid DNA in a high throughput format. By adding a quick endotoxin removal step, the Mag-Bind® Endo-Free plasmid 96 kit provides a flexible and fast solution for isolating endotoxin free plasmid on a small economical scale. The flexible system can be easily automated, thereby making it an ideal option for high throughput transfection screening as well as for other standard molecular biology techniques including restriction enzyme digestion.

Supplier: OMEGA BIOTEK

RNA Wash Buffer II concentrate

Supplier: OMEGA BIOTEK

This dNTP mix with dATP, dCTP, dGTP, dTTP is suitable for use in PCR, sequencing, nick translation, fill-in, cDNA synthesis, and TdT tailing reactions, as well as for dilution of radiolabelled dNTPs.

Supplier: OMEGA BIOTEK

DNA gel loading buffer

Supplier: OMEGA BIOTEK

E.Z.N.A.® MicroElute® Genomic DNA kit provides a rapid and easy method for the isolation of genomic DNA from small sample volumes and sizes for consistent PCR and analytical test results. Using the reversible binding properties of a silica-based material, the speed of mini-column spin technology, and specifically formulated buffers, the system allows the purification of genomic DNA up to 60 kb. Samples are first lysed under denaturing conditions and then applied to the HiBind MicroElute® Column to bind DNA, while cellular debris, haemoglobin, and other proteins are effectively washed away. High quality DNA can be eluted with 10 to 15 µl sterile deionised water or elution buffer from the MicroElute® column.

Supplier: OMEGA BIOTEK

Taq DNA polymerase is a thermostable enzyme that can withstand prolonged incubation at temperatures up to 95°C without significant loss of activity.

Supplier: OMEGA BIOTEK

Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)

Supplier: OMEGA BIOTEK

Hi-Bind® DNA mini columns are silica glass fibre columns that can bind up to 100 μg of genomic DNA or 35 μg of plasmid DNA per prep. Columns feature an attached lid and a vacuum luer for processing with vacuum manifolds and by centrifugation.

Supplier: OMEGA BIOTEK

Isolate DNA from molluscs and insects using spin columns.

Supplier: OMEGA BIOTEK

Binding buffer XP2, Omega Bio-Tek

Supplier: OMEGA BIOTEK

The E.Z.N.A.® Total RNA kit II is designed for isolating total cellular RNA from tissues rich fibrous and fatty tissues such as skeletal muscle, heart, brain and adipose tissues. Compared to other standard silica-column procedures, the E.Z.N.A.® Total RNA kit II provides higher yield and better quality of RNA from all types of tissue. This kit combines phenol/guanidine-base lysis and the silica membrane purification of RNA technology to provide a rapid and easy method of the isolation of total RNA from any tissue sample. RNA purified using the E.Z.N.A.® Total RNA method is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

Supplier: OMEGA BIOTEK

Nucleic Acid Purification Kits and Reagents, MicroElute® DNA columns

Supplier: OMEGA BIOTEK

The Mag-Bind® Stool DNA 96 Kit allows rapid and reliable isolation of high-quality genomic DNA (both pathogen and host DNA) from stool samples.

Supplier: OMEGA BIOTEK

The MicroElute RNA clean-up kit is designed to purify and concentrate RNA (from 50 μg down to picogram amounts) from enzymatic reactions or for desalting RNA samples (e.g. following phenol extraction and ethanol precipitation). The procedure uses lower elution volumes, making it well suited for applications require highly concentrated RNA sample.

Supplier: OMEGA BIOTEK

The E.Z.N.A.® Water DNA kit is formulated to isolate high purity cellular DNA from water samples typically containing humic acid and inhibitors of PCR. This kit has been successfully used to isolate DNA from tough-to-lyse bacteria, fungi, and algae that inhabit a range of samples including tap water, lake, river and sewage samples. Isolated DNA can be used for most downstream applications including PCR, Southern blotting, SNP analysis.

Supplier: OMEGA BIOTEK

Selected protease digestion releases microgram amounts of DNA from FFPE samples.

Supplier: OMEGA BIOTEK

Plasmid isolated with traditional purification procedures normally contain high levels of endotoxins (also known as lipopolysaccharides or LPS) that can significantly interfere with transfection experiments downstream. The E.Z.N.A.® Endo-Free plasmid mini kit II integrates an efficient endotoxin removal step into the plasmid purification procedure to produce high-quality transfection grade (<0,1 EU/µg) plasmid for efficient transfection. The bacterial cells are lysed using the alkaline-SDS lysis method. The cleared cell lysate is then treated with ETR reagent to efficiently remove the endotoxins. After adjusting the binding condition, the cell lysate is applied into the HiBind® DNA column and purified DNA is eluted from the column membrane.

Supplier: OMEGA BIOTEK

The Mag-Bind® oligonucleotide purification kit is designed for the purification of small size DNA fragments (larger than 10 bp) with a 60 to 70% recovery rate. By using the highly efficient binding ability of Mag-Bind® technology, oligonucleotides are selectively bound to the surface of Mag-Bind® particles. Salts and other impurities are washed away with two quick wash steps. Purified oligouncleotide are eluted with deionised water or Elution Buffer and ready for downstream applications.

Supplier: OMEGA BIOTEK

Ribonuclease A

Supplier: OMEGA BIOTEK

The E.Z.N.A.® RNALock Stabilizer Reagent is an innovative reagent which rapidly lyses cells and tissues to inactivate RNases at room temperature. Simply submerge the samples, such as cell cultures, animal tissues, or white blood cells into the RNALock Stabilizer Reagent. The intact cellular RNA will be preserved even at elevated temperature. Samples can be stored in this reagent in excess of 12 months at −20 °C.

Supplier: OMEGA BIOTEK

Mag-Bind® SeqDTR™ is designed for the effective and reliable removal of unincorporated terminators from sequencing reaction. Sequencing products are first mixed with the Mag-Bind® SeqDTR™. DNA then selectively binds to the Mag-Bind® SeqDTR™ particles. With two rapid wash steps, trace contaminants such as nucleotides, primers and small, non targeted amplification products are removed.

Supplier: OMEGA BIOTEK

Isolates total RNA from blood samples stored in special preservation reagents and PAXgene tubes using spin columns.