Supplier: Corning

L-glutamine is an essential amino acid and a key component of culture media, serving as a major energy source for propagating cells. It is very stable as a dry powder and as a frozen solution but degrades rapidly in liquid media or stock solutions, producing toxic compounds. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use. This formulation is prepared in cell culture grade water.

Supplier: Corning

The addition of MEM vitamins to medium can improve growth and viability of propagating cells in culture while reducing the biosynthetic burden on these cells.

Supplier: Corning

FBS is a commonly used cell culture media supplement which helps sustain cell growth and satisfy metabolic requirements.

Supplier: Corning

Corning® cellgro® COMPLETE formulation is based on a proprietary combination of 1:1 DMEM/F12, RPMI and McCoy's 5A medium. This medium contains a mixture of selected trace elements and high molecular weight carbohydrates, extra vitamins, animal origin-free protein, and a small amount of high quality bovine serum albumin (1,0 g/L). The Corning cellgro COMPLETE supports superior growth and viability over long-term passages in hybridomas, along with suspension and adherent cell cultures.

Supplier: Corning

HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulphonic acid) provides an alternative to the use of bicarbonate for buffering because it does not require elevated levels of CO2

Supplier: Corning

This antimycotic interferes with fungal membrane permeability by forming channels in cell membranes, causing small molecules to leak from the cell. It is recommended for use in cell culture applications at 10 ml/L and is stable at 37 ºC for 3 days.

Supplier: Corning

The addition of MEM nonessential amino acids to medium can improve growth and viability of propagating cells in culture while reducing the biosynthetic burden on these cells.

Supplier: Corning

Sodium Acetate is a common molecular biology reagent. All production lots are tested to ensure that they are free of RNAse. It is often used in buffer systems to maintain the pH of a given solution.

Supplier: Corning

Cells grown in traditional media supplemented with L-glutamine are susceptible to toxicity.

Supplier: Corning

Phosphate buffered saline (PBS) is a buffer solution containing sodium chloride, sodium phosphate, and in some formulations potassium chloride and potassium phosphate

Supplier: Corning

Ampicillin is a Penicillin derivative that inhibits bacterial cell-wall synthesis by inactivating transpeptidases on the inner surface of the bacterial cell membrane.

Supplier: Corning

Hink's TNM-FH medium is a modified Grace's formulation containing lactalbumin hydrolysate (LAH) and ultrafiltered yeastolate, and was originally developed for the optimal propagation of cabbage looper, Tricoplusi in cells.

Supplier: Corning

S.O.C

Supplier: Corning

Lymphocyte Separation Medium (LSM) was originally designed for the in vitro isolation of lymphocytes from diluted whole blood

Supplier: Corning

Medium 199 is often used in vaccine production, virology, and for the culture of various non-transformed cells

Supplier: Corning

100 mM Solution with 8.5 g/L NaCl Sodium pyruvate is often used as a component in culture broth and media as an energy source for propagating cells.

Supplier: Corning

This 1:1 mixture of Dulbecco's Modified Eagle Medium (DMEM) and Ham's F-12 media is ideal for culture of a variety of cell types under low serum conditions

Supplier: Corning

Corning® insectagro® DS2 Serum-Free/Protein-Free Medium was developed for the growth and maintenance of Drosophila Schneider 2 (S2) cells to be used in heterologous protein expression

Supplier: Corning

45% Glucose Solution. Glucose is a main source of energy for living organisms and is used as a nutrient source in cell culture media. This 45% (w/v) solution has been to ensure no cytotoxicity.

Supplier: Corning

Corning® cellgro® COMPLETE formulation is based on a combination of 1:1 DMEM/F12, RPMI, and McCoy's 5A medium. This medium contains a proprietary mixture of selected trace elements and high molecular weight carbohydrates, extra vitamins, animal origin-free protein, and a small amount of high quality bovine serum albumin (1,0g/L). The Corning cellgro COMPLETE supports superior growth and viability over long-term passages in hybridomas, along with suspensions and adherent cell cultures.

Supplier: Corning

Corning® cellgro® Albumin is a chemically-defined recombinant human serum albumin (rhHSA). Having an essential and non-essential fatty acid profile, this is a powerful media supplement that outperforms plasma-derived human serum albumin (pHSA), bovine serum albumin (BSA) and FBS. Some prominent applications for cellgro® rhAlbumin are as a supplement for CHO cell culture and for the culture of various other cell lines. This product is plant-derived and completely animal-origin free.

Supplier: Corning

Hygromycin B, produced by Streptomyces hygroscopicus, is used as a selection agent and inhibits protein synthesis in cells not carrying hygromycin phosphotransferase (HPH)

Supplier: Corning

Wide variety of fetal bovine serum (FBS) options to satisfy most common applications and is a perfect complement to Corning classical cell culture media. Products are evaluated for quality, performance
and analytical testing, including sterility, mycoplasma, virus testing, total protein, endotoxin and hemoglobin levels.

Supplier: Corning

Penicillin-Streptomycin-L-glutamine combines two antibiotics with a nutrient which is essential for cell growth, L-glutamine

Supplier: Corning

This product is a mix of the antibiotics Penicillin (5,000 IU) and Streptomycin (5,000 μg/ mL) in a 50-fold working concentration. Penicillin (Penicillin G) works by inhibiting peptidoglycan synthesis, while Streptomycin inhibits protein synthesis. Penicillin-Streptomycin is effective against Gram-negative and Gram-positive bacteria. This antibiotic is recommended for use in cell culture media at 20 ml/L.

Supplier: Corning

G418 Sulphate is recommended for use as a selection agent in a concentration range of 100 to 800 µg/ml. This antibiotic blocks polypeptide synthesis by hindering protein elongation, and inhibits the selection and maintenance of eukaryotic cells stably transfected with the neomycin resistance gene. G418 is an aminoglycoside antibiotic similar in structure to gentamicin, neomycin and kanamycin. This antibiotic is effective against Gram negative and some Gram positive bacteria.

Supplier: Corning

Ciprofloxacin Hydrochloride (HCl) is a 4-fluoroquinolone antibiotic often used to control mycoplasma contamination in infected cell lines

Supplier: Corning

Trypan blue is the most common stain used in cell count and viability assays.

Supplier: Corning

As a natural product, fetal bovine serum contains a wide variety of biological molecules and compounds which can influence cell development.

Supplier: Corning

This is a combination of penicillin, streptomycin and Amphotericin formulated to contain 10000 units/ml penicillin G, 10 mg/ml streptomycin sulphate and 25 µg/ml amphotericin B.