140 Results for: "Biowest"

Supplier: BIOWEST

Amphotericin B is used for the prevention or elimination of fungal contaminants in cell culture.

Supplier: BIOWEST

Antibiotic-antimycotic used in tissue culture to prevent growth of bacteria (gram positive and gram negative), yeast and fungi. Penicillin acts by inhibiting bacterial cell wall synthesis.

Supplier: BIOWEST

Human plasma is processed from human blood that has been coagulation-induced. It is collected from volunteer donors.

Supplier: BIOWEST

Cell culture water is high-quality water suitable for use as a solvent in the preparation of cell culture media and laboratory reagents.

Supplier: BIOWEST

Bovine serum albumin (BSA) is commonly used in cell culture protocols, particularly where protein supplementation is necessary and the other components of serum are unwanted. In cell culture, its main role is as a carrier of small molecules. Because of its negative charge, BSA binds water, salts, fatty acids, vitamins and hormones, then carries these bound components between tissues and cells. The binding capacity also makes BSA an effective scavenger to remove toxic substances, including pyrogens, from the medium.

Supplier: BIOWEST

Accustase is a ready to use cell detachment solution of proteolytic and collagenolytic enzymes. Accutase is a direct gentle replacement for trypsin solution. It can also be used on suspension cells to reduce their agglutination in order to ease counting.

Supplier: BIOWEST

Bovine serum is a pooled serum sample obtained from a normal donor herd. It is specific for anti-bovine serum and yields multiple arcs of precipitation during immunoelectrophoresis versus anti-bovine serum.

Supplier: BIOWEST

D-glucose monohydrate (Dextrose) is a common natural sugar involved in processes such as energy production, glycosylation, and formation of glycans that provide structure to cells. It is involved in a detrimental process in cells called glycation. It is used as a supplement for cell culture and in numerous cellular processes.

Supplier: BIOWEST

Basal Medium Eagle is principally used for monolayer diploid or primary cell cultures. It is the simplest of the basal media with all essential components for growth. BME ideally supports cell lines such as HeLa, L-cells and primary mammalian fibroblasts. BME has also successfully been used for the growth of human transformed cell lines (particularly WI-38 and MRC-5).

Supplier: BIOWEST

Sterile filtered foetal bovine serum (FBS) derived from clotted whole blood aseptically collected from foetus via cardiac puncture. Each manufactured batch is rigorously controlled, from the collection of serum through to final packaging.

Supplier: BIOWEST

Schneider’s Insect Medium was developed to support the growth of excised imaginal discs from the fruit fly, Drosophila melanogaster. When supplemented with 5 to 20% heat-inactivated foetal bovine serum Schneider's mediuam has been found to support the rapid growth of both primary and established cultures of cells derived from Drosophila spp. and several other dipterans.

Supplier: BIOWEST

Donor foal serum is the liquid fraction of whole blood that is collected after the blood is allowed to clot; it is the blood plasma with the fibrinogens removed. Serum includes all proteins not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances. Donor foal serum is used as a blocking agent in immunoassays and controls.

Supplier: BIOWEST

RPMI are general purpose enriched media with extensive applications for a broad spectrum of mammalian and hybridoma cells, including human myeloma, mouse hybridoma, human leukocytes, and B and T lymphocytes. It was originally formulated for suspension cultures and monolayer culture of human leukaemia cells.

Supplier: BIOWEST

Rabbit serum is the liquid fraction of whole blood that is collected after the blood is allowed to clot; it is the blood plasma with the fibrinogens removed. Serum includes all proteins not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances. Rabbit serum is used as a blocking agent in immunoassays and controls.

Supplier: BIOWEST

For immunohistochemistry applications, sheep serum is frequently used as a blocking solution. Using sheep serum is useful for blocking the background cross reactivity with immunoglobulins which are already present in the tissue. When used before application of the primary antibody, sheep serum can eliminate Fc receptor binding of the primary and secondary antibody.

Supplier: BIOWEST

Calf serum is the liquid fraction of whole blood that is collected after the blood is allowed to clot; it is the blood plasma with the fibrinogens removed. Serum includes all proteins not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances. Calf serum is used as a blocking agent in immunoassays and controls.

Supplier: BIOWEST

Plasma provides a broad spectrum of macromolecules, carrier proteins for lipoid substances and trace elements, attachment and spreading factors, low molecular weight nutrients, and hormones and growth factors that promote cell growth. It is used as a component of bioassays, immunoassays or enzyme assays.

Supplier: BIOWEST

Studies to determine the nutritional requirements of many cells have been in progress since Eagle's first reports. The major essential nutrients were identified, and work became focussed on the media requirements of individual cell types. Many media designed for these purposes are now available. Among the first of these media, developed initially to study hormonal requirements of cells in culture, was a mixture of DMEM and Ham's F12 medium, known as DMEM-Ham's F12.

Supplier: BIOWEST

Antibiotic G418 is an aminoglycoside antibiotic similar in structure to gentamycin. It exhibits toxicity towards both eukaryotic and prokaryotic cells. The optimal concentration for selection and maintenance must be determined for each cell line.

Supplier: BIOWEST

Phenol red is used as a pH indicator in cell culture. Its colour exhibits a gradual transition from yellow to red over the pH range 6,8 to 8,2. Above pH 8,2, phenol red turns a bright pink (fuchsia) colour.

Supplier: BIOWEST

Bioguard is used to disinfect surfaces of laboratory apparatus and CO₂ incubators. Bioguard prevents contamination with and growth of fungi, bacteria and other sports, mycoplasma, and viruses including HIV and Hepatitis B.

Supplier: BIOWEST

Ham's F10 medium is intended to support the growth of Chinese Hamster Ovary cells under serum-free conditions and other mammalian cell types with serum supplementation. It is a popular medium for growth of fastidious cell lines.

Supplier: BIOWEST

The Leibovitz L-15 media were formulated to promote the cell growth in medium not balanced in CO₂. The formulations were developed with the sodium bicarbonate buffer. The Leibovitz L-15 media are buffered by a complement of salts, free base amino acids and galactose so they can be used under conditions of free gaseous exchange with the atmosphere. When properly supplemented, L-15 Medium supports established cell lines, such as HEp-2 and LLC-MK2, as well as primary explants of embryonic and adult human tissue. Many viruses also have been successfully cultivated in this medium.

Supplier: BIOWEST

Sterile filtered foetal bovine serum (FBS) derived from clotted whole blood aseptically collected from foetus via cardiac puncture. Each manufactured batch is rigorously controlled, from the collection of serum through to final packaging.

Supplier: BIOWEST

Chicken serum is the liquid fraction of whole blood that is collected after the blood is allowed to clot; it is the blood plasma with the fibrinogens removed. Serum includes all proteins not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances. Chicken serum is used as a blocking agent in immunoassays and controls.

Supplier: BIOWEST

Concentrated solutions of amino acids, vitamins or non essential amino acids can be added to a basal medium to enrich it and the resulting medium used as a classical MEM or BME. The final concentration of the MEM or BME must be 1X.

Supplier: BIOWEST

Gentamicin sulfate acts against gram-negative, gram-positive bacteria and mycoplasma.

Supplier: BIOWEST

Human serum albumin is processed from human blood that has been coagulation-induced. It is collected from volunteer donors.

Supplier: BIOWEST

Porcine serum in lymphocyte culture for chromosomal studies has been utilized and found to be adequate. Moreover, although not commonly used, it is said that the constituents of porcine and human blood are nearly the same. It has also been suggested that porcine serum could be used in setting up peripheral lymphocyte culture for karyotyping.

Supplier: BIOWEST

Gentamycin sulphate acts against Gram-negative, Gram-positive bacteria and mycoplasma.