The qScript™ microRNA cDNA synthesis kit has been designed to convert miRNAs into cDNA starting from total RNA or RNA preparations pre-enriched for miRNAs. miRNAs are not polyadenylated in nature. With the qScript™ microRNA cDNA synthesis kit miRNAs are polyadenylated in a poly(A) polymerase reaction. qScript™ reverse transcriptase and other necessary reagents for cDNA synthesis are subsequently added to convert the poly(A) tailed microRNAs into cDNA using an oligo-dT adapter primer. The adapter primer has a unique sequence at its 5’ end which allows amplification of cDNAs in Real-Time qRT-PCR reactions.

The kit comes complete with a Human Positive Control Primer that can be used to quantitate the small nucleolar RNA SNORD44 which is ubiquitously expressed in most human tissues. In addition, the kit contains 20% extra poly(A) tailing buffer and microRNA cDNA reaction mix to accommodate the use of no poly(A) polymerase and no reverse transcriptase control reactions.