VWR®, TEMPase Hot Start 2× master mix
Supplier: VWR Chemicals
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About this item
TEMPase Hot Start DNA Polymerase Master Mix and Blue TEMPase Master Mix are good alternatives to TEMPase Hot Start DNA Polymerase. These master mixes offer easy reaction assembly at room temperature, reduced set-up time and fewer handling steps, which lead to increased reproducibility. As a consequence TEMPase Hot Start DNA Polymerase Master Mix is highly suited to standard tests.
The blue loading dye in Blue TEMPase Hot Start DNA Polymerase Master Mix facilitates direct gel loading, and eliminates the need for separate loading dye - no need for time-consuming sample preparation before electrophoresis.
Multiplex 2× Master Mix is composed of TEMPase Hot Start DNA Polymerase and a specialised buffer system designed for multiplex PCR.
Delivery: TEMPase Hot Start DNA Polymerase Master Mix and Blue TEMPase Master Mix are available in two variations, either based on Key Buffer (Master Mix K) or Combination Buffer (Master Mix C) to suit different PCR requirements. Additional MgCl₂ is included in the kit to enable optimisation.
Specifications
- Application:Multiplex
- Format:Mastermix incl. dNTPs
- High fidelity:No
- Hot start:Chemically modified
- Storage conditions:– 20 °C (long term), + 4 °C (6 months)
- Amplicon size:≤ 5kb
- Processivity:Standard
- Exonuclease activity:5’→3’
- Product overhang:3'A
- Buffer composition:Based on Ammonium buffer
- Concentration:2x
- Description:TEMPase Hot Start DNA Polymerase 2X Master Mix A
1.5 mM MgCl2 2500 reactions - Size:50 x 1.25 ml
- Units:2500 r Mix 2500
- Features:Direct loading
Specifications
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