You searched for: Nucleic Acid Reagents

Supplier: VWR Chemicals

Ribozol™ RNA Extraction Reagent is a single phase phenol solution optimized for the isolation of total RNA from a variety of cell and tissue types.

Supplier: OMEGA BIOTEK

Isolate total RNA from cells or soft tissues using spin column.

Supplier: OMEGA BIOTEK

MicroElute® Clean Up system, designed for rapid DNA clean up.

Supplier: OMEGA BIOTEK

The E.Z.N.A.® blood DNA kit provides rapid total DNA isolation from up to 250 µl of fresh or frozen anticoagulated whole blood. The E.Z.N.A.® blood DNA kit can also be used for the preparation of genomic DNA from buffy coat, serum, plasma, bone marrow, lymphocytes, platelets, and body fluids. This kit allows for simultaneous processing of single or multiple samples in less than 30 minutes. Phenol/chloroform extractions, and time-consuming steps such as precipitation with isopropanol or ethanol have been eliminated. DNA purified with the E.Z.N.A.® blood DNA method is ready for applications such as PCR, southern blotting, or restriction enzyme digestion.

Supplier: OMEGA BIOTEK

E.Z.N.A.® MicroElute Total RNA Kit provides a rapid and easy method for the isolation of up to 50 µg of total RNA from small amounts of cultured eukaryotic cells, tissues such as as laser dissected samples (LDS) or fine needle aspirates(FNA). Normally, up to 5×10⁵ eukaryotic cells or 5 mg tissue can be used in a single experiment depending on the type of tissue used. This kit allows processing of single or multiple of samples in less than 30 minutes. There is no need for phenol/chloroform extractions, and time-consuming steps such as CsCl gradient ultracentrifuation, and precipitation with isopropanol or LiCl. Purified RNA can be eluted with 10 to 15 µl nuclease-free water. Purified RNA is ready for most downstream applications such as RT-PCR, Northern blotting, Poly A+ purification, nuclease protection and invitro translation.

Supplier: OMEGA BIOTEK

The E.Z.N.A.® tissue DNA kit offers a simple, rapid, and cost effective method for the isolation of DNA from a wide variety of sample sources including fresh or frozen animal cells and tissues. After cell lysis, the DNA purification process can be completed in less than 30 minutes. Up to 30 mg of tissue at a time can be readily processed at a time using the simple E.Z.N.A.® tissue DNA protocol. Single or multiple samples can be simultaneously processed with this spin-column based kit. There is no need for phenol/chloroform extractions, or time-consuming steps such as precipitation with isopropanol or ethanol. DNA purified using the E.Z.N.A.® tissue DNA kit is ready for most downstream applications such as PCR, southern blot and restriction enzyme digestion.

Supplier: VWR Chemicals

RiboZol™ Plus RNA Purification Kit purifies and recovers all sizes and species of RNA, including large RNAs such as ribosomal RNA (rRNA) or messenger RNA (mRNA), and small RNAs <200 nucleotides, including microRNAs (miRNA) and small inhibitory RNAs (siRNA). The kit combines the benefits of organic RNA extraction using RiboZol™ RNA extraction reagent with the ease and convenience of spin column technology. Firstly, RiboZol™ RNA extraction reagent extracts total RNA from cells or tissue, including difficult sample types. Subsequent column chromatography employs a proprietary resin as the separation matrix to yield up to 35 μg of highly purified total RNA.

Supplier: VWR Chemicals

The phenol-free total RNA purification kit provides a rapid method for the isolation and purification of total RNA from cultured animal cells, tissue samples, blood, bacteria, yeast, fungi, plants and viruses.

Supplier: VWR Chemicals

Deoxyribonucleotides are the foundation of DNA and it takes thousands of these nucleotides to make up DNA.

Supplier: VWR Collection

VWR® Delivered High quality Reagents & biochemicals, combined with innovative products that gives optimum results , save time & simplify protocols

Supplier: OMEGA BIOTEK

The E.Z.N.A.® miRNA isolation kit uses a rapid procedure to isolate small RNAs, such as micro RNA (miRNA), small interfering RNA (siRNA), and small nuclear RNA (snRNA), from wide range of tissues and cells. The fast and efficient silica membrane based method isolates total RNA ranging in size from kilo based down to decamers. The kit also provides reagents and a procedure to enrich the population of RNAs that are 200 bases and smaller, which enhances the sensitivity of small RNA detection by solution hybridisation, Northern analysis, and other methods.

Supplier: OMEGA BIOTEK

Isolate DNA from soil and water samples using magnetic beads.

Supplier: Quantabio

Rapid extraction and purification of high-quality total DNA from cultured cells or tissue.

Supplier: OMEGA BIOTEK

Isolate DNA from up to 10 ml blood using magnetic beads.

Supplier: OMEGA BIOTEK

The E.Z.N.A.® Fungal DNA kits allow for the rapid and reliable isolation of high quality total cellular DNA from a wide variety of fungal species without the need for organic extraction.

Supplier: OMEGA BIOTEK

Selected protease digestion releases microgram amounts of RNA from FFPE samples. The purified nucleic acids, although highly fragmented, are suitable for a variety of downstream genomic and gene expression analyses. E.Z.N.A® spin column-based kits are ideal for low throughput applications while Mag-Bind® magnetic beads-based kits are designed specifically for high throughput users with automation capability.

Supplier: OMEGA BIOTEK

The E.Z.N.A.® soil DNA kit is formulated to isolate high purity cellular DNA from soil samples typically containing humic acid and inhibitors of PCR. This kit has been successfully used to isolate DNA from tough-to-lyse bacteria, fungi, and algae that inhabit a range of samples including clay, sandy, peaty, chalky, and loamy soil samples. Isolated DNA can be used for most downstream applications including PCR, southern blotting, and SNP analysis.

Supplier: OMEGA BIOTEK

The E.Z.N.A.® Viral RNA Kit is designed for the isolation of viral RNA from cell free fluids such as plasma, serum, urine and cell culture supernatant. The procedure completely removes contaminants and enzyme inhibitors making viral RNA isolation fast, convenient and reliable. The kit is also suitable for the isolation of total RNA from cultured cells, tissues and bacteria. RNA purified using the E.Z.N.A.® Viral RNA method is ready for all downstream applications such as RT-PCR.

Supplier: OMEGA BIOTEK

This second generation plasmid miniprep kit yields 40 to 70 µg of high-quality DNA in less than 30 minutes using the same format as the E.Z.N.A.® Plasmid Mini Kit I. The increased DNA binding capacity of the HiBind® Mini Column II allows culture volumes of up to 15 ml to be used, thus bridging the gap between mini and midi prep protocols. Plasmid DNA can be readily and quickly isolated for most downstream applications such as routine screening, restriction enzyme digestion, and DNA sequencing.

Supplier: OMEGA BIOTEK

The Mag-Bind® Endo-Free plasmid 96 kit combines the power of Mag-Bind® technology with the innovative ETR technology to deliver high quality endotoxin-free plasmid DNA in a high throughput format. By adding a quick endotoxin removal step, the Mag-Bind® Endo-Free plasmid 96 kit provides a flexible and fast solution for isolating endotoxin free plasmid on a small economical scale. The flexible system can be easily automated, thereby making it an ideal option for high throughput transfection screening as well as for other standard molecular biology techniques including restriction enzyme digestion.

Supplier: OMEGA BIOTEK

The E.Z.N.A.® HP Total RNA Isolation Kit provides a rapid and easy method for RNA isolation from a small amount of cultured eukaryotic cells or tissues. This kit allows single or simultaneous processing of multiple samples in less than 40 minutes. Normally, 1×10⁷ eukaryotic cells or 25 to 30 mg tissue can be used in a single experiment. There is no need for phenol/chloroform extractions, time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl. RNA purified using the E.Z.N.A.® HP Total RNA method is ready for applications such as RT-PCR, RT-qPCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

Supplier: OMEGA BIOTEK

The E.Z.N.A.® Blood DNA Maxi Kit is specially designed for large scale isolation of genomic DNA. The kit provides a rapid purification of genomic DNA from up to 10 ml whole blood samples. Sample sources include fresh and frozen whole blood treated with common anticoagulants such as citrate, EDTA and heparin. In addition plasma, serum, buffy coat, bone marrow, lymphocytes, platelets, and body fluid samples can also be used. Phenol/chloroform extractions, and time-consuming steps such as precipitation with isopropanol have been eliminated. DNA purified using the E.Z.N.A.® Blood DNA Maxi method is free of contaminants and enzyme inhibitors making it suitable for most downstream applications such as PCR, Southern blotting and restriction enzyme digestion of high-quality total DNA.

Supplier: OMEGA BIOTEK

Isolate viral RNA from nasopharyngeal swab specimens (dry or in VTM) using magnetic beads.

Supplier: OMEGA BIOTEK

The E.Z.N.A.® Yeast RNA kit is designed for isolation of high quality total RNA from a wide variety of yeast species. Up to 5×10⁷ log-phase yeast cultures can be processed for each column. Using an enzyme/beads-beating procedure to efficiently break the cell, combined with an optimised cell lysis buffer, the E.Z.N.A.® Yeast RNA kit can purify high-quality of total cellular RNA from various yeast strains.

Supplier: OMEGA BIOTEK

By adapting HiBind® technology with 96-well plates, the E-Z 96® Tissue DNA kit provides a high throughput method to purify genomic DNA from whole blood, buccal swabs, mouse tail, rat tail, tissues and animal cells and tissues in a 96-well plate format. Purified DNA is suitable for most downstream applications such as PCR, restriction enzyme digestion, and hybridisation techniques.

Supplier: OMEGA BIOTEK

The Mag-Bind® Plant DNA DS 96 Kit is designed for difficult samples (DS) which are hard to lyse or contain high amounts of polysaccharides and polyphenols. The system uses a CTAB-based lysis buffer which does not require organic solvents. The proprietary binding system prevents inhibitors from binding to the magnetic beads. The wash buffer system further removes polysaccharides, phenolic compounds and enzyme inhibitors from plant tissue lysate. An optional rebinding step is included with the kit but most samples do not require the additional treatment.

Supplier: OMEGA BIOTEK

E.Z.N.A.® Plasmid Midi Kit offers a novel and reliable method of isolating up to 200 µg of plasmid DNA in less than 60 minutes. This kit combines HiBind® membrane technology with the time-tested consistency of alkaline SDS lysis of bacterial cells to deliver high-quality plasmid DNA. The DNase/RNase-free HiBind® DNA midi columns operate by eliminating time consuming phenol-chloroform extractions and alcohol precipitations. Plasmid purification using the E.Z.N.A.® Plasmid Midi Kit follows a simple bind-wash-elute procedure thereby making it possible for multiple samples to be processed. Although yields vary according to plasmid copy number, E.coli strain, and conditions of growth, 50 ml of an overnight culture in LB medium will typically produce 200 µg of high-copy number plasmid DNA. When working with low-copy number plasmids the starting culture volume can be increased up to 100 ml. High-quality DNA is ready for immediate use in routine molecular biology applications, such as automated fluorescent sequencing, restriction enzyme digestion, and transfection screening.

Supplier: VWR Collection

VWR® Delivered High quality Reagents & biochemicals, combined with innovative products that gives optimum results , save time & simplify protocols

Supplier: OMEGA BIOTEK

Isolates total RNA from blood samples stored in special preservation reagents and PAXgene tubes using spin columns.

Supplier: OMEGA BIOTEK

Isolate total cellular RNA and viral RNA from a wide variety of cells and tissues.