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Cell Culture Buffers

Cell Culture Buffers

Tissue culture buffers provide an energy source, maintain proper pH levels, and keep ideal osmotic pressure present. Used predominately in biomolecular research, the dry and liquid substances have a balanced inorganic salt composition. Lower concentration options permit equilibration with air in a closed system, while higher salt concentration options keep gaseous phases in equilibrium. Cell growth is optimal when plated in a nutritional medium that has been buffered.

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VWR® UltraPure, Buffer, PBS solution 10X concentrate (phosphate buffered saline)

VWR® UltraPure, Buffer, PBS solution 10X concentrate (phosphate buffered saline)

Supplier: VWR Chemicals

Cell culture buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)

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VWR® UltraPure, Buffer, PBS solution (phosphate buffered saline)

VWR® UltraPure, Buffer, PBS solution (phosphate buffered saline)

Supplier: VWR Chemicals

Dulbecco's formulation without calcium and magnesium.

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VWR® Tris Buffer Solution, pH 7,4 (0,1 mol/L) for Biotechnology

VWR® Tris Buffer Solution, pH 7,4 (0,1 mol/L) for Biotechnology

Supplier: VWR Chemicals

Cell culture buffer, TRIS buffer solution pH 7,4 (0,1 mol/l)

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VWR®, Phosphate buffered saline (PBS) 1X, for biotechnology

VWR®, Phosphate buffered saline (PBS) 1X, for biotechnology

Supplier: VWR Chemicals

137 mM sodium chloride, 2,7 mM potassium chloride, 12 mM phosphate buffer

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VWR® UltraPure, Phosphate buffered saline (PBS), powdered

VWR® UltraPure, Phosphate buffered saline (PBS), powdered

Supplier: VWR Chemicals

PBS, Ultra Pure Grade is a powdered, magnesium- and calcium-free formulation of PBS suitable for cell culture and for use in flow cytometry applications.

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Cell culture salts, salt solutions and buffers

Cell culture salts, salt solutions and buffers

Supplier: BIOWEST

These irrigating buffers and salt solutions are sterile, physiological balanced solutions intended for use in the maintenance of mammalian cells, where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro. Applications include the intermediate steps for cultivation of cells, like washing, centrifugation, suspending and counting, as well as for many analytical methods or biochemical treatments. Principally these solutions should be used to keep the cells in suspension only for minutes or a few hours maximum. These buffers and solutions are not cell culture media.

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Hank's balanced salt solutions (HBSS), Corning®

Hank's balanced salt solutions (HBSS), Corning®

Supplier: Corning

Hanks' Balanced Salt Solution (HBSS) is a buffer used to maintain a physiological pH for cells maintained in non-CO₂ atmospheric conditions.

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Phosphate-buffered saline (PBS), Corning®

Phosphate-buffered saline (PBS), Corning®

Supplier: Corning

Phosphate buffered saline (PBS) is a buffer solution containing sodium chloride, sodium phosphate, and in some formulations potassium chloride and potassium phosphate

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Dulbecco's phosphate-buffered saline (DPBS), Corning®

Dulbecco's phosphate-buffered saline (DPBS), Corning®

Supplier: Corning

Dulbecco's Phosphate Buffered Saline (DPBS) is a buffer solution which can be used to maintain cell culture media in the physiological pH range of 7,0 to 7,6

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Phosphate-Buffered saline (PBS), 10X concentrate, pH 7,4, molecular biology grade, Corning®

Phosphate-Buffered saline (PBS), 10X concentrate, pH 7,4, molecular biology grade, Corning®

Supplier: Corning

Phosphate Buffered Saline (PBS) is used to maintain a physiological pH and osmolarity, two key factors in tissue culture and molecular biology research

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Buffer, HEPES solution (1 mol/L), Corning®

Buffer, HEPES solution (1 mol/L), Corning®

Supplier: Corning

HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulphonic acid) provides an alternative to the use of bicarbonate for buffering because it does not require elevated levels of CO2

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Sodium acetate, Corning® 3M™

Sodium acetate, Corning® 3M™

Supplier: Corning

Sodium Acetate is a common molecular biology reagent. All production lots are tested to ensure that they are free of RNAse. It is often used in buffer systems to maintain the pH of a given solution.

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Sodium bicarbonate, Corning®

Sodium bicarbonate, Corning®

Supplier: Corning

Sodium bicarbonate is used as part of a buffering system commonly used to maintain physiological pH 7,2 to 7,4 in a culture environment

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10X Tris buffered saline, liquid, Corning®

10X Tris buffered saline, liquid, Corning®

Supplier: Corning

Tris Buffered Saline (TBS) is an isotonic, non-toxic buffer used in some biomedical techniques to maintain pH within a relatively narrow range.

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