You searched for: Extraction Buffers
Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.
VWR® Lysis Buffer, Hypotonic
Supplier: VWR Chemicals
Detergent-free buffer for isolation of proteins from tissue culture cells. Cell lysis and the subsequent isolation of the crude cytoplasmic fraction completed in less than 30 m.
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J.T.Baker® Cell Lysis Solution, Ultrapure Bioreagent
Supplier: Avantor
J.T. Baker® Cell Lysis Solution. Optimized multi-component, ready-to-use solution for extraction and recovery of AAV particles from HEK293 or SF9 cells. The product offers quantitative recovery through efficient cell lysis and protection of vector particles against shear stress. The solution is formulated with non-ionic detergents which are octyl phenol ethoxylate free.
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J.T.Baker® Cell Lysis Solution, Biotech Reagent
Supplier: Avantor
J.T.Baker® Cell Lysis Solution Biotech Reagent is a cGMP pre-formulated and ready to use detergent optimized for the extraction and recovery of AAV particles from producer cell lines (HEK293 parental and suspension lines, SF9). J.T.Baker® Cell Lysis Solution is a proprietary blend of nonionic surfactants, free of octyl phenol ethoxylate, which demonstrates comparable performance industry standard detergents while addressing environmental concerns associated with REACH and downstream clearance concerns. Designed for Gene Therapy workflows, this formulation is easy to use and handle as it is ready-to-use, has low viscosity, does not require cold temperature shipping or storage, and is available in sizes to support processes from development to large scale commercial batches. Detail description provided in technical literature.
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E.Z.N.A.® RNALock stabilizer reagent
Supplier: OMEGA BIOTEK
The E.Z.N.A.® RNALock Stabilizer Reagent is an innovative reagent which rapidly lyses cells and tissues to inactivate RNases at room temperature. Simply submerge the samples, such as cell cultures, animal tissues, or white blood cells into the RNALock Stabilizer Reagent. The intact cellular RNA will be preserved even at elevated temperature. Samples can be stored in this reagent in excess of 12 months at −20 °C.
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Genomic DNA extraction buffers
Supplier: OMEGA BIOTEK
P1 buffer, Omega Bio-Tek
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Lysis buffer, CTAB
Supplier: PanReac AppliChem
Cationic detergent cetyltrimethylammonium bromide (CTAB) is used to liberate and complex with total cellular nucleic acids.
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CSPL buffer
Supplier: OMEGA BIOTEK
Component of the Mag-Bind® plant DNA 96 plus kit.