Size exclusion analysis of mAbs: advanced detection - advanced columns
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Monoclonal antibodies (mAbs) are routinely analyzed by size exclusion chromatography to detect high and low molecular weight impurities (mAb aggregates or fragments). Predominantly, UV absorbance is used for detection. However, advanced detection technologies namely multiangle light scattering (MALS) and mass spectrometry (MS) better characterize mAbs and reveal species hidden in UV chromatograms. This webinar will explain the different detection methods and their results. Special emphasis will be placed on the higher requirements for size exclusion columns preceding advanced detection methods.
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Presented by
Andrea Krumm
Andrea Krumm studied biotechnology before gaining a PhD in cancer biology. After four years as an applications specialist for optical analytical devices used in life science research, she joined Tosoh Bioscience GmbH in 2020 as a product manager for analytical columns. As such she is responsible for gathering customer requirements, application areas and addressing these with new and existing products of the analytical columns line.