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1909 results for "nucleic acid & pcr amplification"

1909 Results for: "nucleic acid "

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Software for spectrometers

Supplier: BIOCHROM

This software is supplied in different modules to meet the application requirements of different customer groups and to offer a simple upgrade path should your requirements change.

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PCR plates, 384-well

PCR plates, 384-well

Supplier: Brand

PP. The 40 μl wells can hold sample volumes of 2 to 30 μl. This helps to reduce reagent costs and shortens cycle times. The plates can be filled using multichannel pipettes or robotic systems.

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Filter plates, 24-well, AcroPrep™

Filter plates, 24-well, AcroPrep™

Supplier: Cytiva (Formerly Pall Lab)

The Acroprep 24-well filter plate utilises Pall’s proprietary high performance multi-layer filter media and membranes to offer time savings, strong performance claims and streamlined workflow improvements in a 24-well plate format.

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Agarose, white powder, high resolution for molecular biology

Supplier: MP Biomedicals

Agarose High Resolution is an intermediate melting agarose with approximately twice the resolution capability of routine agarose. It is a purified linear galactan hydrocolloid isolated from agar or agar-bearing marine algae. It is ideal for the resolution of small DNA fragments in the range 20 bp to 2000 bp.
Agarose high resolution is an intermediate melting and gelling point agarose which exhibits superior sieving characteristics. It is recommended for analytical gels of DNA fragments under 1000 bp and is ideal for small DNA fragments down to 20 bp can be separated on 6 % gels. It resolves fragments differing in size by only 2 bp. Its use avoids the need for PAGE Southern and Northern transfers can be achieved efficiently from them at concentrations up to 2%. DNA fragments can be purified with high efficiency from this agarose gels up to 4% by using either a purification kit or an electroelution system.

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EDC-HCl (N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride), Pierce™

EDC-HCl (N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride), Pierce™

Supplier: Thermo Fisher Scientific

Thermo Scientific Pierce EDC is a carboxyl- and amine-reactive zero-length crosslinker. EDC reacts with a carboxyl group first and forms an amine-reactive O-acylisourea intermediate that quickly reacts with an amino group to form an amide bond with release of an isourea by-product. The intermediate is unstable in aqueous solutions and so two-step conjugation procedures rely on N-hydroxysuccinimide (NHS) for stabilization. Failure to react with an amine will result in hydrolysis of the intermediate, regeneration of the carboxyl, and release of an N-substituted urea. A side reaction is the formation of an N-acylurea, which is usually restricted to carboxyls located in hydrophobic regions of proteins.

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Amersham™ Protran® Western Blotting Membranes, Nitrocellulose

Amersham™ Protran® Western Blotting Membranes, Nitrocellulose

Supplier: Cytiva

Amersham™ Protran® membranes are manufactured from 100% pure nitrocellulose to ensure the highest binding capacity possible. They are compatible with a variety of detection methods, including isotopic, chemiluminescent, colorimetric and fluorescent. No methanol pre-wetting step is required, making this the membrane of choice for proteins which prefer aqueous environments. Prior to transfer the membrane is simply wetted with water, and then placed in the transfer buffer. No other pretreatment steps are necessary.

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Eppendorf Tubes® 3810X Microtubes 1,5 ml

Eppendorf Tubes® 3810X Microtubes 1,5 ml

Supplier: EPPENDORF

Eppendorf Tubes® 3810X microtubes (1,5 ml) are designed for seamless one-handed operation with an easy-to-open, securely closing tube design. Perfect for sample preparation, centrifugation, and storage, these tubes will effortlessly integrate into workflows for efficient and reliable performance.

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Sample Collection Cards, LeafSaver

Supplier: Ahlstrom-Munksjö

This LeafSaver is ideal for simple collection, transportation and ambient-temperature storage of plants, fungi, insects and parasites.

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PMA enhancer for Gram negative bacteria solution 5X

Supplier: Biotium

PMA Enhancer for Gram Negative Bacteria was designed to improve PMA- and PMAxx™-mediated discrimination between live and dead gram-negative bacteria.

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Microplate readers, multi-mode, SpectraMax® i3x

Microplate readers, multi-mode, SpectraMax® i3x

Supplier: Molecular Devices

The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel detection capabilities without the need for service engineers or costly downtime - it grows with you as your research evolves.

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DENARASE® highly active endonuclease from Serratia marcescens

DENARASE® highly active endonuclease from Serratia marcescens

Supplier: C LECTA

DENARASE® is a highly active endonuclease from Serratia marcescens. This enzyme is used in biopharmaceutical manufacturing to eliminate plasmid and host cell DNA/RNA. DENARASE® is manufactured under GMP conditions and is widely applied during the purification of viral vectors for gene/cell therapies and vaccines.

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Affinity chromatography media, Heparin Sepharose™ 6 Fast Flow

Affinity chromatography media, Heparin Sepharose™ 6 Fast Flow

Supplier: Cytiva

Heparin Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified sodium heparin. Immobilization is performed using chemically stable and oriented coupling giving high binding capacity. The chemical stability of the media is solely dictated by the heparin ligand. Heparin Sepharose 6 Fast Flow is a member of the Cytiva range of affinity chromatography media for capture and intermediate purification.

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PCR plates for qPCR, 24-well and 48-well

PCR plates for qPCR, 24-well and 48-well

Supplier: Brand

PP, non skirted. Manufactured under cleanroom conditions, and made from pure, high quality materials (without additives) these PCR plates are suitable for use with most commercially available thermal cyclers.

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EDC-HCl (N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride), No-Weigh™ Format, Pierce™

Supplier: Thermo Fisher Scientific

Thermo Scientific Pierce EDC is a carboxyl- and amine-reactive zero-length crosslinker. EDC reacts with a carboxyl group first and forms an amine-reactive O-acylisourea intermediate that quickly reacts with an amino group to form an amide bond with release of an isourea by-product. The intermediate is unstable in aqueous solutions and so two-step conjugation procedures rely on N-hydroxysuccinimide (NHS) for stabilization. Failure to react with an amine will result in hydrolysis of the intermediate, regeneration of the carboxyl, and release of an N-substituted urea. A side reaction is the formation of an N-acylurea, which is usually restricted to carboxyls located in hydrophobic regions of proteins.

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Dual-Cooled Vertical Electrophoresis Systems, SE 600 Ruby™

Dual-Cooled Vertical Electrophoresis Systems, SE 600 Ruby™

Supplier: Cytiva

The key to outstanding, reproducible electrophoresis results is temperature control. Gels unequally cooled on their two faces produce slanted bands appearing as broad or double bands in stained gels. SE 600 Ruby™ ensures uniform heat in gels because, firstly, the gels are completely immersed in the lower buffer for uniform heat transfer and, secondly, a standard magnetic stirrer circulates buffer around the vertically suspended heat exchanger to maintain homogeneous lower buffer temperature. The ergonomically designed SE 600 Ruby™ provides two built-in side handles, which facilitates carrying and handling.

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DNA extraction and purification kit

DNA extraction and purification kit

Supplier: Q BIOANALYTIC

Successful PCR applications depend on well purified DNA. In diagnostics and analytics of food and medical samples PCR and real-time PCR has many advantages over conventional methods. In all cases it is of importance to have well purified DNA for subsequent PCR, real-time PCR, hybridization or sequencing purposes. Purified DNA leads to higher sensitivity and also specificity of PCR based tests in difficult matrices. With QuickBlue Extraction and Purification Kit you always get high DNA yields and highly purified DNA. The kit uses paramagnetic nano-particles for purification. It can help to purify DNA from difficult matrices like food or even blood in soil for applications in forensic sciences. DNA in Seawater can also be purified. This method avoids extensive centrifugation steps and can thus be applied in environments where centrifuges are not usable. The kits includes proteinase K as a reagent for cell disruption, so that extraction and purification can be performed with the kit. DNA yield from 1 ml bacterial enrichment is 10 μg of Genomic DNA. The protocol can be adapted to automated magnetic bead separation instruments.

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Microplate readers, Varioskan™ LUX

Microplate readers, Varioskan™ LUX

Supplier: THERMO LABSYSTEMS LIFE SCIENCE

Varioskan™ LUX comes equipped with a range of measurement technologies including absorbance, fluorescence intensity and FRET as standard, and with optional luminescence, AlphaScreen and time-resolved fluorescence (TRF) modules. The instrument selects the measurement wavelength using filters or monochromators, depending on which is optimal for each measurement technology.

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AxyPrep Mag Viral NA-96 Prep 1 * 1 items

Supplier: Corning

AxyPrep Mag Viral NA-96 Prep 1 * 1 items

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AxyPrep Mag Viral NA-384 Prep (for 4 96-well plates) 1 * 1 items

Supplier: Corning

AxyPrep Mag Viral NA-384 Prep (for 4 96-well plates) 1 * 1 items

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