306555 Results for: "buffers and cell culture grade water hyclone"

Supplier: SIGMA ALDRICH MICROSCOPY

Bromothymol blue has been used for freshwater pH measurements over a broad range of buffer intensities.

Supplier: VWR Chemicals

Microbiology media in bags are ideally suited for high sample volumes.

Supplier: HyClone products (Cytiva)

DMEM Low Glucose was the original modification of Basal Medium Eagle (BME) used to grow mouse embryonic cells. DMEM, Low Glucose is suitable for the culturing of many immortalised cell lines from humans, monkeys, hamsters, rats, mice, chicken and fish. DMEM contains four times more amino acids, vitamins and other supplementary components than Basal Medium Eagle (BME), the base media from which DMEM was derived. As DMEM contains no growth-promoting agents or proteins, supplementation with FBS is recommended for optimal performance.

Supplier: MP Biomedicals

Tweens® are a series of nonionic surfactants derived from sorbitan esters. They are soluble or dispersible in water but differ widely in organic and oil solubilities. Used as oil-in-water emulsifiers in pharmaceuticals, cosmetics, cleaning compounds, etc.
Tween® 80 has been widely used in biochemical applications including: solubilizing proteins, isolating nuclei from cells in culture selective protein extraction growing of tubercule bacilli, and emulsifying and dispersing substances in medicinal and food products. It has little or no activity as an anti-bacterial agent. It has been shown to have an adverse effect on the antibacterial effect of methyl paraben and related compounds. Non-ionic detergent used for selective protein extraction and isolation of nuclei from mammalian cell lines.
Soluble/miscible in water to give a clear yellow solution; miscible with alcohol, dioxane, and ethyl acetate; practically insoluble in liquid paraffin and fixed oils (such as mineral oil).
Autoclaving of solutions is not recommended. Sterile filtering is suggested with a 0.22 micron filter. Tween® may need to be warmed to about 40 °C and alternated with portions of hot distilled water while being poured through the filter.
Store at Room Temperature (15-30 °C).

Supplier: Thermo Fisher Scientific

The Nunc™ Edge plate has a design that minimises evaporation and the risks associated with the edge effect. When filled with sterile water or media, the surrounding moat acts as an evaporation buffer during extended incubation, yielding consistent, viable cells across the entire 96-well plate that in turn enhances productivity for cell-based assays. Enabling the use of all 96 wells allows researchers to plan experiments more efficiently, resulting in potentially less cost and related product waste.

Supplier: MP Biomedicals

Storage: Store at Room Temperature (15-30 °C)
Sodium chloride is a commonly used chemical which is found widely in nature. It is considered to be an essential nutrient. Excess amounts of sodium chloride can destroy electrolyte balance and cause death in most animals, including humans.
Sodium chloride is used in a wide variety of biochemical applications, including intravenous fluids (normal saline is 0.9% w/v in water), density gradients, a diluent to increase ionic strength in buffers or culture media and in salt-out procedures in the isolation of DNA. It has also been used in high concentrations for preservation of foods since bacteria cannot grow in high salt conditions. Sodium chloride is widely used in protein crystallization.

Supplier: ENZO LIFE SCIENCES

Wash Buffer Salts can be used in tissue preparations, immunohistochemical staining procedures and in assays that require HRP-linked detection reagents.

Supplier: THERMO FISHER DIAGNOSTICS

Cell culture buffer, Phosphate buffered saline tablets

Supplier: SIGMA ALDRICH MICROSCOPY

Bromophenol blue has been used in the sample buffer prepared for western blotting. A tracking dye for nucleic acid or protein electrophoresis in agarose or polyacrylamide gels.

Supplier: BIOWEST

These irrigating buffers and salt solutions are sterile, physiological balanced solutions intended for use in the maintenance of mammalian cells, where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro. Applications include the intermediate steps for cultivation of cells, like washing, centrifugation, suspending and counting, as well as for many analytical methods or biochemical treatments. Principally these solutions should be used to keep the cells in suspension only for minutes or a few hours maximum. These buffers and solutions are not cell culture media.

Supplier: VWR Chemicals

Detergent-free buffer for isolation of proteins from tissue culture cells. Cell lysis and the subsequent isolation of the crude cytoplasmic fraction completed in less than 30 m.

Supplier: THERMO FISHER DIAGNOSTICS

Dry-Bags take the hassle out of media preparation. They remove the need for glassware, making them a safe and convenient option for food-testing laboratories and eliminate the need for washing of glassware and heat sterilization. Furthermore, there is no need to wait for hot media to cool down. Once water is added and powder dissolved, Dry-Bags are ready to use immediately. When empty, the bag is simply disposed of with normal laboratory waste.

Supplier: VWR Chemicals

Biological buffers are useful for cell culture in vitro, enzyme assays and some electrophoretic applications at physiological pH.

Supplier: LIOFILCHEM

Buffered NaCl Peptone Solution pH 7,0 is used for dissolving, suspending and diluting test samples. Its composition conforms to recommendations in the Harmonised Pharmacopoeias for the microbiological examination of non-sterile products.

Supplier: VWR Chemicals

Biological buffers are useful for cell culture in vitro, enzyme assays and some electrophoretic applications at physiological pH.

Supplier: VWR Chemicals

Medium 199 was developed specifically for nutritional research of chicken fibroblasts. Today, 199 media are widely used for the maintenance of non transformed cells, vaccine and virus production and primary explants of epithelial cells. The media formulated with the Hanks salts are buffered with saline solutions designed for balancing in ambient conditions, and their use in a CO₂ incubator results in a rapid drop in pH of the culture medium.

Supplier: G-Biosciences

The PhosphataseArrest™ phosphatase inhibitor cocktails are ready to use 100X solutions that are simply added to either extraction buffers or samples.

Supplier: VWR Collection

Square PETG bottles are ideal for storage of media, laboratory reagents and biological buffers up to −70 °C. Eliminate washing, depyrogenation and autoclaving steps with sterile and non-pyrogenic.

Supplier: Merck

Synergy® ultrapure water systems are ideal for laboratories with access to purified water that needs a 'point of use' supply of high quality ultrapure water. The Synergy® system is suitable for users needing 2 to 20 L/day. The system produces Type I water from pre-treated water at a flow from 1 to 1,5 L/min. The water produced is suitable for the preparation of buffers for liquid chromatography, biochemical experiments, isocratic or gradient solutions for HPLC analysis, culture media for bacteriological analysis or cell cultures and solutions for spectrophotometry, spectroscopy and other analytical techniques.

Supplier: VWR Chemicals

Every MediaBag T (tube-bag) is filled with pre-weighed granulated media. Connect the 0,2 μm filter unit aseptically to the tube by using the connector device. Add water under aseptic conditions into the bag via the filter system. Protect the tube end with the red cap.

Supplier: VWR Chemicals

A range of culture media in compliance with ISO standards and the International Harmonised Pharmacopoeia. All our media are, as you would expect, produced using high quality raw materials and are subject to strict quality controls, in a production environment following Good Manufacturing Practice guidelines. Raw materials are selected in compliance with the required standards. Dehydrated culture media should be stored at 10 to 30 °C unless otherwise stated, in their closed containers in a dry environment. Standard pack size is 500 g.

Supplier: THERMO FISHER DIAGNOSTICS

ReadyBags™ are designed for use in food testing by laboratories with large numbers of samples requiring large volumes of broth media or diluent. Each ReadyBag™ contains 3 liters and is intended for use with an automatic dispenser. The ReadyBag™ is connected to tubing for standard laboratory equipment, such as gravimetric diluters or peristaltic pumps, by means of a connector. Connectors are available in two formats: autoclavable, re-usable stainless steel connectors or sterile, plastic, single-use connectors.

Supplier: VWR Chemicals

Biological buffers are useful for cell culture in vitro, enzyme assays and some electrophoretic applications at physiological pH.

Supplier: ENZO LIFE SCIENCES

For the quantitative determination of sPLA2 in culture supernatants, cell lysates, and buffers from any species.

Supplier: Apollo Scientific

Glycerine, anhydrous, Cell Culture Grade

Supplier: Apollo Scientific

Dimethyl sulphoxide, Cell Culture Grade

Supplier: BIOWEST

The Leibovitz L-15 media were formulated to promote the cell growth in medium not balanced in CO₂. The formulations were developed with the sodium bicarbonate buffer. The Leibovitz L-15 media are buffered by a complement of salts, free base amino acids and galactose so they can be used under conditions of free gaseous exchange with the atmosphere. When properly supplemented, L-15 Medium supports established cell lines, such as HEp-2 and LLC-MK2, as well as primary explants of embryonic and adult human tissue. Many viruses also have been successfully cultivated in this medium.

Supplier: HyClone products (Cytiva)

Defined foetal bovine serum (FBS) is the highest quality of FBS available and is very widely used by those cell culturist who have a concern for viral contaminants and require an extensive biochemical profile. Defined FBS is filtered through multiple 40 nm (0,04 µm) pore-size related filters, which are the most retentive filters used in commercial FBS production. Over 50 components are analysed and the results are included on the biochemical assay.

Supplier: PROTIDE PHARMACEUTICALS

Material grade - USP, Ph. Eur.

Supplier: VWR Collection

VWR® cell slides are made from medical grade, high transparency PET, which has high strength and is unbreakable. Only the surface of the cell slide has a hydrophilic treatment so adherent cells grow easily, whilst the surrounding culture dish surface is hydrophobic, making it difficult for adherent cells to grow.