HyClone™ CDM4CHO, CHO Cell Culture Platform Liquid Media
Supplier: HyClone products (Cytiva)
HyQ® CDM4CHO™ is a chemically-defined protein-free medium containing no animal derived components. It is designed to increase the process yields for the industrial manufacture of recombinant proteins using a variety of CHO cell clones. HyQ® CDM4CHO™ has been successfully tested in various culture systems, including T-flasks, shaker flasks and bioreactors. HyQ® CDM4CHO™ contains Pluronic® F68 and L-glutamine, and does not contain phenol red.
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Cell culture media, liquid, Iscove's Modified Dulbecco's Medium (IMDM), HyClone™
Supplier: HyClone products (Cytiva)
HyClone Classical Liquid Media: Iscove's Modified Dulbeccos Medium (IMDM) is manufactured using ISO 9001:2000-certified processes.
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HyClone™ MEM (modified), Cell culture media, powdered
Supplier: HyClone products (Cytiva)
Dry powdered media for supporting cell culture growth and biomanufacturing processes.
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HyClone™ Ham's F-12 Basal Media, Cell Culture Media, Liquid
Supplier: HyClone products (Cytiva)
Ham's F-12 media was originally derived by modifying Ham's F-10 to include increased concentrations of choline, inositol, putrescine, and several amino acids. It was developed specifically for serum free culturing of Chinese Hamster Ovary (CHO) cells and has also been used with supplementation to grow chondrocytes, primary rat hepatocytes, rat prostate epithelial cells, carcinoma cells, rat skeletal myoblasts and rat, rabbit, and chicken embryos.
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Insect cell culture media, serum-free, CCM3, HyClone™
Supplier: HyClone products (Cytiva)
HyClone™ CCM3 media is designed to increase cell yields with multiple lines in a variety of culture environments. It is tailored for Sf9 cells.
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HyClone™ Ham's Nutrient Mixture F10/F12, Cell Culture Media, Powdered
Supplier: HyClone products (Cytiva)
Dry powdered media for supporting cell culture growth and biomanufacturing processes.
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CHO cell culture platform liquid media, HyClone™ HyCell™ TransFx-C
Supplier: HyClone products (Cytiva)
HyCell™ TransFx-C Media is a hydrolysate-free and regulatory-friendly media which supports transient transfection, growth, and expression of a variety of recombinant proteins in CHO cells.
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CHO cell culture platform liquid media, HyClone™ PF-CHO LS and PF-CHO MPS
Supplier: HyClone products (Cytiva)
HyClone™ PF-CHO LS and PF-CHO MPS media are designed to support the DHFR selection/amplification system. They have been successfully tested in a variety of cell culture systems, including T-flasks, spinner flasks, and bioreactors. PF-CHO MPS media is the powder version of PF-CHO LS media.
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Cell culture media, Dulbecco's Modified Eagles Medium (DMEM), High Glucose, HyClone™
Supplier: HyClone products (Cytiva)
HyClone™ DMEM high glucose liquid media supports cell growth in research, upstream process development, and cell culture manufacturing processes.
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HyClone Cell Boost™ 3 supplement
Supplier: HyClone products (Cytiva)
Supplements cell culture with amino acids, vitamins, glucose, and trace elements and manufactured to meet cGMP manufacturing standards and QC specifications.
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HyClone Cell Boost™ 4 supplement
Supplier: HyClone products (Cytiva)
Supplements cell culture with amino acids, vitamins, glucose, trace elements, and growth factors. Manufactured to meet cGMP manufacturing standards and QC specifications.
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HyClone Cell Boost™ 1 supplement
Supplier: HyClone products (Cytiva)
Supplements cell culture with amino acids, vitamins, and glucose and manufactured to meet cGMP manufacturing standards and QC specifications.
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HyClone Cell Boost™ 5 supplement
Supplier: HyClone products (Cytiva)
Supplements cell culture with lipids, amino acids, vitamins, and growth factors. Manufactured to meet cGMP manufacturing standards and QC specifications.
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HyClone Cell Boost™ 2 Supplement
Supplier: HyClone products (Cytiva)
Supplements cell culture with amino acids and glucose and manufactured to meet cGMP manufacturing standards and QC specifications.
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HyClone Cell Boost™ 6 supplement
Supplier: HyClone products (Cytiva)
Supplements cell culture with lipids, amino acids, vitamins, and growth factors. Manufactured to meet cGMP manufacturing standards and QC specifications.
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L-Glutamine, HyClone™
Supplier: HyClone products (Cytiva)
HyClone™ L-glutamine is used as a supplement in serum- and protein-free media. It supports the growth of cells that have high energy demands and synthesise large amounts of proteins and nucleic acids.
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HyClone Cell Boost™ 7a supplement
Supplier: HyClone products (Cytiva)
Supplements cell culture with amino acids, vitamins, salts, trace elements, and glucose. Manufactured to meet cGMP manufacturing standards and QC specifications.
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VWR®, Cell culture media, Medium 199
Supplier: VWR Chemicals
Medium 199 was developed specifically for nutritional research of chicken fibroblasts. Today, 199 media are widely used for the maintenance of non transformed cells, vaccine and virus production and primary explants of epithelial cells. The media formulated with the Hanks salts are buffered with saline solutions designed for balancing in ambient conditions, and their use in a CO₂ incubator results in a rapid drop in pH of the culture medium.
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VWR®, Cell Slides in Sulture Dishes
Supplier: VWR Collection
VWR® cell slides are made from medical grade, high transparency PET, which has high strength and is unbreakable. Only the surface of the cell slide has a hydrophilic treatment so adherent cells grow easily, whilst the surrounding culture dish surface is hydrophobic, making it difficult for adherent cells to grow.
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Cell culture media, Liebovitz L-15 medium
Supplier: BIOWEST
The Leibovitz L-15 media were formulated to promote the cell growth in medium not balanced in CO₂. The formulations were developed with the sodium bicarbonate buffer. The Leibovitz L-15 media are buffered by a complement of salts, free base amino acids and galactose so they can be used under conditions of free gaseous exchange with the atmosphere. When properly supplemented, L-15 Medium supports established cell lines, such as HEp-2 and LLC-MK2, as well as primary explants of embryonic and adult human tissue. Many viruses also have been successfully cultivated in this medium.
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Yeast extract, molecular biology grade
Supplier: PanReac AppliChem
Water-soluble part of autolysed yeast. Rich of ammonia and vitamins, especially of water-soluble vitamin B-complexes; suitable for addition to media.
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HyClone™ SFM4CHO media
Supplier: HyClone products (Cytiva)
HyClone™ SFM4CHO media is a serum-free and protein-free formulation that contains no components of bovine origin and has been designed for high performance in a variety of culture vessels, including bioreactors.
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Trypsin Protease, HyClone™
Supplier: HyClone products (Cytiva)
Dissociation reagents, such as trypsin, are used to detach and disaggregate adherent cells from the surface or substrate to which they are attached, as well as for dissociating tissues for primary cell culture.
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HyClone Cell Boost™ 7b supplement
Supplier: HyClone products (Cytiva)
Supplements cell culture with concentrated amino acids. Manufactured to meet cGMP manufacturing standards and QC specifications.
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Water, Cell Culture Grade, tested to USP specifications for injection, sterile, Corning®
Supplier: Corning
Corning® Cell Culture Grade Water Tested to USP Sterile Water for Injection Specifications. Cell culture grade water is high-quality water suitable for use as a solvent in the preparation of cell culture media and other reagents for sensitive applications.
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L-alanyl L-glutamine, SG-200, HyClone™
Supplier: HyClone products (Cytiva)
SG-200 solution is a stable dipeptide of L-alanyl L-glutamine.
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Cell culture media, Iscove's Modified Dulbecco's Medium (IMDM)
Supplier: BIOWEST
In 1976, Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a reduced-serum medium supplemented with albumin, transferrin, lecithin, and selenium. Iscove's medium is a modification of Dulbecco's Modified Eagle's Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies have demonstrated that Iscove's medium will support murine B lymphocytes, haemopoietic tissue from bone marrow, B-cells stimulated with lipopolysaccharide, T-lymphocytes, and a variety of hybrid cells.
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Murashige and skoog Medium with vitamins and sucrose
Supplier: Apollo Scientific
Modified version of product number PMM519, contains 30 g/L D-Sucrose. Plant Tissue Culture Tested. Soluble in water.
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VWR®, Cell culture media, Minimum Essential Medium (MEM)
Supplier: VWR Chemicals
Minimum Essential Medium (MEM) with Earle’s Balanced Salts is a modification of Eagle’s earlier Basal Medium (BME) which contains a higher concentration of essential nutrients. These media promote the growth of a variety of normal and transformed cells. Since they contain Earle’s Balanced Salts, they are suitable for use in atmospheres charged with CO₂ gas.
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HyClone™ DMEM/F12 1:1 Cell Culture Media
Supplier: HyClone products (Cytiva)
HyClone™ DMEM/F12 is an equal parts blend of DMEM and Ham’s F12 media that is widely used for culturing many different mammalian cells including MDCK, Glial cells, fibroblasts, and endothelial cells. This formulation combines the high concentration of glucose, amino acids and vitamins from DMEM with the wide variety of other components available in Ham’s F-12.