306713 Results for: "buffers and cell culture grade water hyclone"

Supplier: HyClone products (Cytiva)

Designed for vaccine manufacturing using suspension cell culture with avian cell line.

Supplier: HyClone products (Cytiva)

AdvanceSTEM™ Mesenchymal Stem Cell Basal Medium and Mesenchymal Stem Cell Growth Supplement have been formulated to support expansion and maintenance of undifferentiated human mesenchymal stem cells (hMSCs).

Supplier: HyClone products (Cytiva)

ADCF medium for use with hybridoma and myeloma cell lines.

Supplier: HyClone products (Cytiva)

HyClone™ SFX-Insect™ with L-glutamine is a versatile protein-free cell culture medium developed through metabolic pathway design to support the growth of multiple insect cell lines and production of a variety of recombinant proteins. It provides superior growth of many key insect cell lines, including Sf9, Sf21, and High Five (T.ni), requiring minimal adaptation.

Supplier: HyClone products (Cytiva)

Ready-to-use medium supports high cell growth, exceptional transfection efficiency, and robust viral production for adeno-associated virus and lentivirus expressed in human embryonic kidney (HEK293) cell lines.

Supplier: HyClone products (Cytiva)

HyCell™ TransFx-C Media is a hydrolysate-free and regulatory-friendly media which supports transient transfection, growth, and expression of a variety of recombinant proteins in CHO cells.

Supplier: HyClone products (Cytiva)

HyQ® CDM4CHO™ is a chemically-defined protein-free medium containing no animal derived components. It is designed to increase the process yields for the industrial manufacture of recombinant proteins using a variety of CHO cell clones. HyQ® CDM4CHO™ has been successfully tested in various culture systems, including T-flasks, shaker flasks and bioreactors. HyQ® CDM4CHO™ contains Pluronic® F68 and L-glutamine, and does not contain phenol red.

Supplier: HyClone products (Cytiva)

HyClone™ SFM4 insect is a versatile cell culture medium developed through Metabolic Pathway Design to support the growth of multiple insect cell lines and production of a variety of recombinant proteins without compromising performance. HyClone SFM4 insect provides superior growth of many key insect cell lines, including Sf9, Sf21, High Five (T.ni), and D.mel.

Supplier: HyClone products (Cytiva)

Protein-free and animal-derived component-free medium

Supplier: HyClone products (Cytiva)

HyClone™ SFM4CHO-Utility media is ideally suited for the cost-effective manufacturing of recombinant proteins for academic and industrial research, genomics and proteomics, drug target screening and validation, and manufacturing of preclinical lots. It is formulated using a proprietary lipid complexing process for enhanced stability and broad-spectrum growth promotion.

Supplier: HyClone products (Cytiva)

HyClone™ HyCell™ TransFx-H media supports transient transfection, growth, and expression of a variety of recombinant proteins in HEK 293 cells. It is a high-performing, animal-derived component-free (ADCF) cell culture media with the versitility to be used with a variety of HEK 293 cell lines in microlitre volumes starting from 200 µl to production-scale volumes in bioreactors.

Supplier: HyClone products (Cytiva)

Supplements cell culture with lipids, amino acids, vitamins, and growth factors. Manufactured to meet cGMP manufacturing standards and QC specifications.

Supplier: HyClone products (Cytiva)

HyClone™ PF-CHO LS and PF-CHO MPS media are designed to support the DHFR selection/amplification system. They have been successfully tested in a variety of cell culture systems, including T-flasks, spinner flasks, and bioreactors. PF-CHO MPS media is the powder version of PF-CHO LS media.

Supplier: HyClone products (Cytiva)

Enhance cell culture growth with U.S.-sourced Cytiva HyClone Super Low IgG Fetal Bovine Serum (FBS).

Supplier: HyClone products (Cytiva)

Minimum Essential Medium (MEM) is one of the earliest modifications of Basal Medium Eagle (BME) containing amino acid concentrations that more closely mimic those found in mammalian cells. MEM has been used with serum supplementation on a broad range of cell types including suspension and adherent mammalian cells, HeLa, BHK-21, HEK-293, HEp-2, HT-1080, MCF-7,fibroblasts and primary rat astrocytes.

Supplier: HyClone products (Cytiva)

Dissociation reagents, such as trypsin, are used to detach and disaggregate adherent cells from the surface or substrate to which they are attached, as well as for dissociating tissues for primary cell culture.

Supplier: HyClone products (Cytiva)

SG-200 solution is a stable dipeptide of L-alanyl L-glutamine.

Supplier: HyClone products (Cytiva)

Ham's F-12 media was originally derived by modifying Ham's F-10 to include increased concentrations of choline, inositol, putrescine, and several amino acids. It was developed specifically for serum free culturing of Chinese Hamster Ovary (CHO) cells and has also been used with supplementation to grow chondrocytes, primary rat hepatocytes, rat prostate epithelial cells, carcinoma cells, rat skeletal myoblasts and rat, rabbit, and chicken embryos.

Supplier: VWR Chemicals

A biological buffer used for cell culture, in vitro, enzyme assays and some electrophoretic applications at physiological pH.

Supplier: HyClone products (Cytiva)

Nuclease-free HyClone Molecular Biology-Grade Water, containing no DEPC or other carcinogenic chemicals is used for critical applications involving nucleotides such as RNA and DNA.

Supplier: HyClone products (Cytiva)

With HyrTryp™ cell dissociation reagent, an animal-derived component-free (ADCF) recombinant alternative to porcine trypsin, you can dissociate and harvest cells with confidence. Simply substitute it in your existing protocol.

Supplier: MP Biomedicals

Hanks' balanced salts solution is used to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions.

Supplier: VWR Chemicals

Buffered peptone water in a safe and light plastic bottle; just shake and add your sample.

Supplier: STEMCELL Technologies

Dulbecco’s phosphate-buffered saline (D-PBS) can be used as a temporary diluting, washing, irrigating or transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media and provides cells with a source of water and essential inorganic ions.

Supplier: Corning

Phosphate Buffered Saline (PBS) is used to maintain a physiological pH and osmolarity, two key factors in tissue culture and molecular biology research

Supplier: VWR Chemicals

Dehydrated culture media in small sachets ready to make up exactly 500 ml of medium.

Supplier: Corning

Corning® Cell Culture Grade Water Tested to USP Sterile Water for Injection Specifications. Cell culture grade water is high-quality water suitable for use as a solvent in the preparation of cell culture media and other reagents for sensitive applications.

Supplier: Corning

Sodium Dodecyl Sulfate (SDS) is an anionic detergent with applications in SDS-PAGE protein analysis and nucleic acid purification. It is a wetting agent that is effective in both acid and alkaline solutions, and is most often used as a protein and lipid solubilization reagent. SDS is also a powerful protein denaturant. This 10% solution is prepared in 18 megaohm water and is 0.2µm filtered.

Supplier: STEMCELL Technologies

Dulbecco’s phosphate-buffered saline (D-PBS) can be used as a temporary diluting, washing, irrigating or transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media and provides cells with a source of water and essential inorganic ions.

Supplier: Corning

EDTA (ethylenediaminetetraacetic acid) is a polyamino carboxylic acid. It is a colorless, water-soluble solid and is useful as a chelating agent due to its ability to "sequester" metal ions such as Ca2+ and Fe3+.  These cations are responsible for various undesirable effects in molecular biological and cell culture processes. After being bound by EDTA, these metal ions remain in the solution but exhibit diminished reactivity.  The use of EDTA therefore allows for greater control over many experiments.