1070 Results for: "SIGMA ALDRICH MICROSCOPY"

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Leucocrystal Violet has been used to identify histamine-producing lactobacilli in cheese.

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Carmine is a cochineal product usually furnished as an aluminum and calcium salt of carminic acid. Carminic acid is used for human-medical cell diagnosis and serves the purpose of the histological investigation of sample material of human origin. It is suitable for staining nuclei in histological sections. It is utilised in various staining methods including the procedure for detection of glycogen and mucous substances (Best′s carmine), for nuclear staining, or vital staining.

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Thymol blue sodium salt in ACS Reagent grade water 95% (dye content), Sigma-Aldrich®

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Methylene Violet 3RAX has been used as a reagent in a preparation of citrate methylene violet, which is used for staining yeast cells. It is also used as an internal standard in tandem mass spectrometry (MS/MS) for the quantification of methylene blue in blood sample.

Supplier: SIGMA ALDRICH MICROSCOPY

1-(Methylamino)anthraquinone 98%, Sigma-Aldrich®

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Fluorescein diacetate (FDA) is a non-fluorescent molecule, which is hydrolysed to fluorescent fluorescein in live cells. Dead cells cannot accumulate or hydrolyse FDA. Fluorescein diacetate has been used for the differentiation of live cells from dead cells.

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bisBenzimide H 33258 has been used for nuclear staining in cells. bisBenzimide H 33258 is useful for staining DNA, chromosomes and nuclei. bisBenzimide H 33258 may be used for fluorescence microscopy or flow cytometry.

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Tetrazolium blue chloride (BTC) is a colorless tetrazolium compound that upon reduction forms a colored diformazan. The reactivity has been applied to the analysis of reducing compounds including the nanomolar detection of reducing sugars and carbidopa and methyldopa. BTC has been used for histological staining of mouse coronal brain sections and rat brain sections. In combination with 4-nitrocatechol, tetrazolium blue chloride forms a ternary complex with molybdenum(VI) for extraction and spectrophotometric determination of molybdenum. It has also been used to stain Francisella tularensis cells for tularemic microagglutination test.

Supplier: SIGMA ALDRICH MICROSCOPY

3-(2-Pyridyl)-5,6-diphenyl-1,2,4-triazine-4',4"-disulfonic acid sodium salt is also known as ferrozine.

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Xylenol orange tetrasodium salt is a fluorochrome acid dye with complexing and chelating properties that make it useful as an indicator in the spectrophotometric detection and assay of metal ions such as calcium, cadmium, bismuth, zinc, lead, and mercury. Its tetrasodium salt especially is used as an indicator in metal titrations. It changes color from yellow to red between pH 6.4 to 10.4. It has six potentially anionic substituents, one being sulfonate, and is anionic under staining conditions and during most laboratory applications.

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Purpurin (1, 2, 4-trihydroxyanthraquinone) is the key pigment present in the roots of Indian madder (Rubia cordifolia). This pigment produces colors, with distinctive heat and light resistant properties.

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Reagent used for the generation of chemiluminescence in the presence of a reductant and oxygen; it is employed in numerous chemiluminescent assays. Lucigenin is specific for superoxide anion radical.

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Brilliant Blue R concentrate is a methanol based stain especially designed for staining protein in polyacrylamide but will also stain agarose gels. The stain contains methanol and acetic acid so gels do not require fixing prior to staining. Brilliant Blue R Concentrate has been used for protein band detection after nondenaturing gradient gel electrophoresis, isoelectric focussing and SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). For detection of protein bands following electrophoresis.

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Acid Fuchsin calcium salt,certified by the Biological Stain Commission, Dye content ≥60 % (Acid Magenta; Acid Rubin; Fuchsin S, SS, SS, ST, or SIII; Rubin S)

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Oil Red O stains lipid materials and imparts red-orange color to the lipids. It also marks vacuoles, particularly observed in ALL-L3 (acute lymphoblastic leukemia) or Burkitt′s lymphoma/leukemia. Oil Red O staining is done on fresh samples, as alcohol fixation removes lipids.

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Used to differentiate viable cells from apoptotic cells. Live cells that are not apoptotic accumulate 6-carboxyfluorescein but do not accumulate annexin. Fluorescent wavelengths λex 492 nm; λemm 517 nm in 0.1 M Tris, pH 8.0.

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5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride has been used for labeling of yeast cells to study the adhesion of yeast cells to the digestive tract of sea bass (Dicentrarchus labrax) larvae and leopard grouper (Mycteroperca rosacea).

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p-Xylene-bis(N-pyridinium bromide). ≥95% (TLC) (DPX)

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3,3′-dihexyloxacarbocyanine iodide (DiOC6) is a supravital lipophilic fluorochrome. This cationic dye is employed to stain mitochondria and endoplasmic reticulum in animal and plant cells. In addition, it acts as a very rapid, consistent and highly selective fluorochrome for endophytic ascomycetes in plant roots and liverworts. DiOC6 also facilitates the detection of different mitochondrial changes happening during early apoptosis.

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Naphthol yellow S has been used as a staining agent in fixed human embryonic stem cell-derived cardiomyocytes.

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Gill No. 1 formulation is used as a progressive cytology stain. Used with hematoxylin and eosin staining.

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Amaranth has been used as a dye for the microscopic visualization of crop contraction in blowflies. It has been used in the electrophoresis gel to track the migration front of the electrode buffer.

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Evans blue has been used to check cell viability. It has been used to study BBB permeability.

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3-Ethoxy-4-methoxybenzaldehyde is a derivative of vanillin. 3-Ethoxy-4-methoxybenzaldehyde may be used to synthesise Compound of Designation red 10 binding (CDr10b) to investigate its role in neuroinflammatory diseases.

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Hartman′s fixative, which is also known as Davidson′s Fixative, contains acetic acid, alcohol, and formalin. It is widely used for the preservation of eye, bone marrow, breast, testis, and other tissues for histologic examination

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Thionin(acetate), Sigma-Aldrich®

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Suitable for use as a protease substrate.

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Bathophenanthrolinedisulfonic acid disodium salt hydrate has been used to measure iron to determine the solubility of iron in the biological sample. It has been used as an iron chelator to grow plants without Fe-EDTA (ethylenediaminetetraacetic acid).

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Calcein blue fluorescent dye, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

4,4′-Methylenebis(N,N-dimethylaniline) is an intermediate in dye manufacture and works as a reagent for the determination of lead. It is suitable for the qualitative assay of cyanogens.