28464 Results for: "G-Biosciences"

Supplier: G-Biosciences

TRIS HCl (tris(hydroxymethyl)aminomethane hydrochloride)

Supplier: G-Biosciences

OmniPrep™ for Mouse Tail Kit isolates high quality genomic DNA from mouse tail samples. If used according to the protocols this kit purifies DNA from 100 to 200 cm mouse tail.

Supplier: G-Biosciences

Following digestion of proteins with proteases, the addition of Pep-Extract™ rapidly elutes digested peptides in under 20 minutes. The eluted peptides can be used directly in mass spectrometry analysis without any additional clean up. Contains sufficient for 500 protein spots.

Supplier: G-Biosciences

FOCUS™ Extraction Buffers are experimentally optimised concentrations of critical agents, buffering and stabilising agents, including urea, thiourea, Nonidet® P-40, CHAPS, and sulphobetaines (SB). They are designed to produce optimal protein extraction and improved spot resolution for 2D gel analysis.

Supplier: G-Biosciences

Trypsin is a serine endopeptidase that specifically cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues and typically there is little or no cleavage at arginyl-proline and lysyl-proline bonds. The distribution of these residues in proteins allows trypsin digestion to produce peptides that are readily identified by mass spectrometry.

Supplier: G-Biosciences

InGel™ Array is a 96-well format kit suitable for processing larger numbers of protein spots concurrently and is compatible with spot-picking instruments. Fewer than 96 samples a batch may also be processed without any waste.

Supplier: G-Biosciences

Specifically designed for plant research and supplied with plant specific reagents, including reagents for removal of plant pigments and other natural products that may interfere with protein analysis. Extracts and solubilises nearly all of the proteins from plants, including membrane as well as soluble proteins. Supplied with a strong proprietary chaotropic extraction buffer to solubilise even the most difficult proteins.

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: G-Biosciences

Fast Yeast Transformation™ is a rapid single step yeast transformation kit that takes less than 10 minutes to prepare competent yeast cells. The competent yeast cells can be used immediately or frozen for later use. This method is suitable for both circular and linear plasmid transformations.

Supplier: G-Biosciences

Extracts and solubilises nearly all of the proteins from insect cell cultures (i.e. Sf9 and Sf21), including membrane as well as soluble proteins, using a strong chaotropic extraction buffer to solubilise even the most difficult proteins.

Supplier: G-Biosciences

Calcium chloride 1 M in aqueous solution

Supplier: G-Biosciences

Ellman's reagent is a versatile, water-soluble compound for quantifying free sulfhydryl groups in solution. It reacts with a free sulfhydryl group to yield a mixed disulfide and 2-nitro-5-thiobenzoic acid (NTB), a measurable yellow colored product at 412nm.
Ellman's reagent is very useful as a free sulfhydryl assay reagent due to its high specificity for -SH groups at neutral pH, high molar extinction coefficient and short reaction time.

Supplier: G-Biosciences

Sulfo SASD Sulfosuccinimidyl-2-(p-azidosalicylamido)ethyl-1,3-dithiopropionate

Supplier: G-Biosciences

The conjugation and modification reactions used to cross-link proteins or couple labels to proteins, such as biotin, enzymes, and fluorescent dyes, require certain conditions, including pH and chemical composition for optimal conjugation. Many common buffers routinely used in laboratories have an inhibitory effect on conjugation reactions; for example, Tris buffers inhibit coupling to amines.

Supplier: G-Biosciences

Anti-IgG Goat Antibody (AP (Alkaline Phosphatase))

Supplier: G-Biosciences

G-Biosciences' Botanical Garden Blot is prepared using total protein extracted from a variety of 5 to 10 day old, whole plant tissues.

Supplier: G-Biosciences

Anti-IgG Goat Antibody (HRP (Horseradish Peroxidase))

Supplier: G-Biosciences

EDC is a heterobifunctional, water-soluble, zero-length carbodiimide crosslinker that is used to couple carboxyl groups to primary amines. EDC activates carboxyl groups first and forms amine reactive O-acylisourea imtermediate that spontaneously reacts with primary amines to form an amide bond and isourea by-product.

Supplier: G-Biosciences

N-Sulfosuccinimidyl(4-iodoacetyl)aminobenzoate Heterobifunctional Cross Linkers.

Supplier: G-Biosciences

3,3’-Dithiobis(sulfosuccinimidyl propionate) Homobifunctional Cross Linker.

Supplier: G-Biosciences

Anti-IgG Rabbit Antibody (HRP (Horseradish Peroxidase))

Supplier: G-Biosciences

m-Maleimidobenzoyl-N-hydroxysulfosuccinimide esterHeterobifunctional Cross Linker

Supplier: G-Biosciences

N-(ρ-Maleimidophenyl) isocyanate Heterobifunctional Cross Linkers.

Supplier: G-Biosciences

Sulfodisuccinimidyl tartrate Homobifunctional Cross Linker.

Supplier: G-Biosciences

Disuccinimidyl suberate Homobifunctional Cross Linker.

Supplier: G-Biosciences

Apoptosis is commonly defined as programmed mechanism of cell death and is a highly regulated process that can be initiated by a variety of internal and external stimuli (inducers).

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: G-Biosciences

N-Octyl-β-D-glucopyranoside

Supplier: G-Biosciences

Dithiothreitol (DTT, Cleland's reagent)

Supplier: G-Biosciences

Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)