Supplier: Biotium

Membrane-permeant AM ester form of Fluo-3 that can be loaded into cells via incubation. Fluo-3 AM ester itself does not bind Ca2 , but it is readily hydrolyzed to Fluo-3 by endogenous esterases once the dye is inside the cells. An important application of Fluo-3 AM ester is its use in high throughput drug screening.

Supplier: Biotium

Membrane-permeant AM ester form of Fluo-3 that can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, the mild detergent Pluronic F-127 (catalog no. 59004) is often used as a dispersing agent to facilitate loading. Fluo-3 AM ester itself does not bind Ca2 , but it is readily hydrolyzed to fluo-3 by endogenous esterases once the dye is inside the cells. An important application of Fluo-3 AM ester is its use in high throughput drug screening.

Supplier: Enzo Life Sciences

Calcium dye

Supplier: Biotium

Membrane-permeant form of Fluo-4 calcium indicator.

Supplier: AAT Bioquest

Calcium measurements are critical for numerous biological investigations.

Supplier: AAT Bioquest

Calcium measurement is critical for numerous biological investigations.

Supplier: AAT Bioquest

Calcium measurement is critical for numerous biological investigations.

Supplier: AAT Bioquest

The cell-permeant Mag-Fluo-4 AM is an analog of Fluo-4 AM with a Kd for Mg ion of 4.7 mM and a Kd for Ca ion of 22 µM, making it useful as an intracellular Mg ion indicator as well as a low-affinity Ca ion indicator.

Supplier: AAT Bioquest

Calcium measurement is critical for numerous biological investigations.

Supplier: AAT Bioquest

Calcium measurement is critical for numerous biological investigations.

Supplier: AAT Bioquest

Calcium measurements are critical for numerous biological investigations.

Supplier: AAT Bioquest

Calcium measurements are critical for numerous biological investigations.

Supplier: AAT Bioquest

Fluo-5F is an analog of Fluo-4 with lower calcium-binding affinity (Kd = ~2.3 µM), making it suitable for detecting intracellular calcium levels in the range of 1 µM to 1 mM that would saturate the response of Fluo-4.

Supplier: AAT Bioquest

Fluo-5N is an analog of Fluo-4 with lower calcium-binding affinity (Kd = ~90 uM), making it suitable for detecting intracellular calcium levels in the range of 1 µM to 1 mM that would saturate the response of Fluo-4.

Supplier: AAT Bioquest

Calcium measurements are critical for numerous biological investigations.

Supplier: SIGMA ALDRICH MICROSCOPY

Fura 2-AM (Fura 2) is a calcium-specific ratio-metric fluorescent dye. It is also known as acetoxy-methyl-ester. In response to calcium ions, ratio metric dyes like Fura 2 changes their respective excitation or emission spectra. The intracellular calcium concentration is determined by the fluorescence emission ratio or excitation at distinct wavelengths. Fura 2 has an emission peak at 505 nm, and the excitation peak ranges between 340 to 380 nm and is the preferred calcium indicator.

Supplier: Merck

AMP (2-Amino-2-methylpropanol) for synthesis, Sigma-Aldrich®

Supplier: Thermo Fisher Scientific

AMP (2-Amino-2-methylpropanol), (max. 5% H₂O) 95%

Supplier: Thermo Fisher Scientific

AMP (2-Amino-2-methylpropanol) 99%

Supplier: SIGMA ALDRICH MICROSCOPY

Calcein AM 4 mM in DMSO ≥90% (by HPCE), clear solution

Supplier: Cayman Chemical

Calcein AM is a cell-permeable dye that upon entering live cells is cleaved by intracellular esterases, leaving membrane-impermeant calcein.

Supplier: VWR Chemicals

5% water has been added to maintain liquidity at room temperature.

Supplier: Biotium

Fluo-3, pentapotassium is membrane-impermeant, but can be loaded into cells via microinjection or scrape loading.

Supplier: Enzo Life Sciences

PKC activator

Supplier: Enzo Life Sciences

Cannabinoid receptor ligand.

Supplier: Enzo Life Sciences

Cannabinoid receptor ligand.

Supplier: Enzo Life Sciences

Anandamide transport inhibitor

Supplier: Enzo Life Sciences

HNMPA-(AM)3 is a cell-permeable analog of HNMPA. It inhibits insulin receptor tyrosine kinase activity (IC50=100 µM) and insulin-stimulated glucose oxidation in isolated rat adipocytes (IC50=10 µM). It has no effect on PKA (at concs. up to 1 mM) or PKC (at concs. up to 420 µM). Inhibits both serine and tyrosine autophosphorylation by the human insulin receptor. Does not affect protein kinase C (PKC) or cAMP-dependent protein kinase (PKA).

Supplier: Enzo Life Sciences

CB1 receptor ligand.

Supplier: Enzo Life Sciences

Retinoic acid analog which acts as a selective RARα agonist with no affinity for RXRs. Induces differentiation in leukemia cell lines and inhibits angiogenesis in chick embryos.